1887

Abstract

The varicella-zoster virus (VZV) glycoprotein B (gB) is a major viral antigen which elicits immunity and neutralizing antibodies. In this study, the genomic map position and DNA sequence of a simian varicella virus (SW) homologue of the VZV gB gene was identified and the transcript analysed. A P-labelled VZV gB DNA probe hybridized to a subclone of the SW B restriction endonuclease fragment indicating the fine map position of SW DNA sequences homologous to the VZV gB gene. The SW gB DNA sequence was determined and analysis revealed a 2751 base pair open reading frame (ORF) with 71·1% identity to the VZV gB gene and 53·8% identity to the herpes simplex type 1 gB gene. The SW gB ORF encodes a 916 amino acid polypeptide with a predicted molecular mass of 104K. The deduced SW and VZV gB polypeptides share 78·9 % amino acid identity and predicted TV-linked glycosylation sites, cleavage sites and transmembrane regions. P-labelled SW gB DNA and RNA probes hybridized to a 3·5 kilobase SW polyadenylated transcript. Primer extension experiments identified transcript start sites for the SW and VZV gB genes and permitted a comparison of the sequences upstream of the SW and VZV gB ORFs. The SW and VZV gB promoter elements are similar in content and align closely. The VZV gB transcript start site suggests a gB polypeptide initiation site which is inconsistent with the previously reported ATG start codon.

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1994-11-01
2021-09-24
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References

  1. Albrecht J. C., Fleckenstein B. 1990; Structural organization of the conserved gene block of herpesvirus saimiri coding for DNA polymerase, glycoprotein B, and major DNA binding protein. Virology 174:533–542
    [Google Scholar]
  2. Ayres J. P. 1971; Studies of the delta herpesvirus isolated from the patas monkey (Erythrocebus patas). Laboratory Animal Science 21:685–695
    [Google Scholar]
  3. Blakely G. A., Lourie B., Morton W. G., Evans H. H., Kaufman A. F. 1973; A varicella-like disease in macaque monkeys. Journal of Infectious Diseases 127:617–625
    [Google Scholar]
  4. Borchers K., Weigelt W., Buhk H. -J., Ludwig H., Mankertz J. 1991; Conserved domains of glycoprotein B (gB) of the monkey virus, simian agent 8, identified by comparison with herpesvirus gBs. Journal of General Virology 72:2299–2304
    [Google Scholar]
  5. Busslinger M., Portman R., Irminger J., Birnsteil M. 1980; Ubiquitous and gene-specific regulatory 5′ sequences in a sea urchin histone DNA clone coding for histone protein variants. Nucleic Acids Research 8:957–978
    [Google Scholar]
  6. Bzik D. J., Fox B. A., Deluca N. A., Person S. 1984; Nucleotide sequence specifying the glycoprotein gene, gB, of herpes simplex virus type 1. Virology 133:301–314
    [Google Scholar]
  7. Cai W., Gu B., Person S. 1988; Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. Journal of Virology 62:2596–2604
    [Google Scholar]
  8. Cantin E. M., Eberle R., Baldick J. L., Moss B., Willey D. E., Notkins A. L., Openshaw H. 1987; Expression of the herpes simplex virus 1 glycoprotein B by a recombinant vaccina virus and protection of mice against lethal herpes simplex virus 1 infection. Proceedings of the National Academy of Sciences, U.S.A 84:5908–5912
    [Google Scholar]
  9. Clarke P., Rabkin S. D., Inman M. V., Mahalingam R., Cohrs R., Wellish M., Gilden D. H. 1992; Molecular analysis of simian varicella virus DNA. Virology 190:597–605
    [Google Scholar]
  10. Clarkson M. J., Thorpe E., McCarthy K. 1967; A virus disease of captive vervet monkeys Cercopithicus aethiops caused by a new herpes virus. Archiv für die gesamte Virusforschung 22:219–234
    [Google Scholar]
  11. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  12. Felsenfeld A. D., Schmidt N. J. 1977; Antigenic relationships among several simian varicella-like viruses and varicella-zoster virus. Infection and Immunity 15:807–812
    [Google Scholar]
  13. Felsenfield A. D., Schmidt N. J. 1979; Varicella-zoster virus immunizes patas monkeys against simian varicella-like disease. Journal of General Virology 42:171–178
    [Google Scholar]
  14. Feng D. -F., Doolittle R. F. 1987; Progressive sequence alignment as a prerequisite to correct phylogenetic trees. Journal of Molecular Evolution 25:351–360
    [Google Scholar]
  15. Fletcher T. M. III Gray W. L. 1992; Simian varicella virus: characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster virus. Journal of General Virology 73:1209–1215
    [Google Scholar]
  16. Fletcher T. M. III Gray W. L. 1993; DNA sequence and genetic organization of the unique short (Us) region of the simian varicella virus genome. Virology 193:762–773
    [Google Scholar]
  17. Gannon F., O’Hare K., Perrin F., Lepennee J., Benoist C., Cochet M., Breathnach R., Garapin A., Cami B., Chambon P. 1979; Organization and sequence at the 5′ end of a cloned complete ovalbumin gene. Nature; London: 278428–434
    [Google Scholar]
  18. Giller R. H., Winistorfer S., Grose C. 1989; Cellular and humoral immunity to varicella zoster virus glycoproteins in immune and susceptible human subjects. Journal of Infectious Diseases 160:919–928
    [Google Scholar]
  19. Gray W. L., Oakes J. E. 1984; Simian varicella virus DNA shares homology with human varicella-zoster virus DNA. Virology 136:241–246
    [Google Scholar]
  20. Gray W. L., Baumann R. P., Robertson A. T., O’Callaghan D. J., Staczek J. 1987; Characterization and mapping of equine herpesvirus type 1 immediate early, early, and late transcripts. Virus Research 8:233–244
    [Google Scholar]
  21. Gray W. L., Pumphrey C. Y., Ruyechan W. T., Fletcher T. M. 1992; The simian varicella virus and varicella zoster virus genomes are similar in size and structure. Virology 186:562–572
    [Google Scholar]
  22. Gray W. L., Gusick N., Fletcher T. M., Pumphrey C. Y. 1993; Characterization and mapping of simian varicella virus transcripts. Journal of General Virology 74:1639–1643
    [Google Scholar]
  23. Hammerschmidt W., Conraths F., Mankertz J., Pauli G., Ludwig H., Buhk H. J. 1988; Conservation of a gene cluster including glycoprotein B in bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1). Virology 165:388–405
    [Google Scholar]
  24. Keller P. M., Davison A. J., Lowe R. S., Bennett C. D., Ellis R. W. 1986; Identification and structure of the gene encoding gpll, a major glycoprotein of varicella-zoster virus. Virology 152:181–191
    [Google Scholar]
  25. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  26. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  27. Larkin M., Heckels J. E., Ogilvie M. M. 1985; Antibody response to varicella-zoster virus surface glycoproteins in chickenpox and shingles. Journal of General Virology 66:1785–1793
    [Google Scholar]
  28. McCarthy K., Thorpe E., Laursen A. C., Heymann C. S., Beale A. J. 1968; Exanthematous disease in patas monkeys caused by a herpes virus. Lancet ii:856–857
    [Google Scholar]
  29. McGeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
    [Google Scholar]
  30. Massaer M., Haumont M., Place M., Bollen A., Jacobs P. 1993; Induction of neutralizing antibodies by varicella-zoster virus gpll glycoprotein expressed from recombinant vaccinia virus. Journal of General Virology 74:491–494
    [Google Scholar]
  31. Navarro D., Paz P., Pereira L. 1992; Domains of herpes simplex virus I glycoprotein B that function in virus penetration, cell-to-cell spread, and cell fusion. Virology 186:99–112
    [Google Scholar]
  32. Oakes J. E., D’Offay J. M. 1988; Simian varicella virus. In Virus Diseases in Laboratory and Captive Animals pp. 163–174 Darai G. Edited by Boston: Martinus Nijhoff;
    [Google Scholar]
  33. Pumphrey C. Y., Gray W. L. 1992; The genomes of simian varicella virus and varicella zoster virus are colinear. Virus Research 26:255–266
    [Google Scholar]
  34. Rasile L., Ghosh K., Raviprakash K., Ghosh H. P. 1993; Effects of deletions in the carboxy-terminal hydrophobic region of herpes simplex virus glycoprotein gB on intracellular transport and membrane anchoring. Journal of Virology 67:4856–4866
    [Google Scholar]
  35. Riopelle A. J., Ayres J. P., Siebold H. R., Wolf R. H. 1971; Studies on primate diseases at the Delta Center. In Medical Primatology pp. 826–838 Goldsmith E. I., Moor-Jankowski J. Edited by New York: Albert Phiebig Inc;
    [Google Scholar]
  36. Robbins A. K., Dorney D. J., Wathen M. W., Whealy M. E., Gold C., Watson R. J., Holland L. E., Weed S. D., Levine M., Glorioso J. C., Enquist L. W. 1987; The pseudorabies virus gpll gene is closely related to the gB glycoprotein gene of herpes simplex virus. Journal of Virology 61:2692–2701
    [Google Scholar]
  37. Sanchez-Pescador L., Paz P., Navarro D., Pereira L., Kohl S. 1992; Epitopes of herpes simplex virus type 1 glycoprotein B that bind type-common neutralizing antibodies elicit type-specific antibody-dependent cellular cytotoxicity. Journal of Infectious Diseases 166:623–627
    [Google Scholar]
  38. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  39. Sarmiento M., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. VI. Conformation of the virion glycoprotein designated vp7 (B2). Journal of Virology 29:1159–1167
    [Google Scholar]
  40. Soike K. F. 1992; Simian varicella virus infection in African and Asian monkeys. The potential for development of antivirals for animal diseases. Annals of the New York Academy of Sciences 653:323–333
    [Google Scholar]
  41. Takasaka M. I., Sakakibara R., Suzuki M. 1990; An outbreak of nonhuman primate varicella-like herpesvirus infection in the established breeding colony of cynomologus monkeys. Tsukuba Primate Center News 9:5–9
    [Google Scholar]
  42. Vafai A. 1993; Antigenicity of a candidate varicella-zoster virus glycoprotein subunit vaccine. Vaccine 11:937–940
    [Google Scholar]
  43. Whalley J. M., Robertson G. R., Scott N. A., Hudson G. C., Bell C. W., Woodworth L. M. 1989; Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB. Journal of General Virology 70:383–394
    [Google Scholar]
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