1887

Journal of General Virology header logo

Volume 75, Issue 11

Lethal Mutations Within the Conserved Stem-loop of African Cassava Mosaic Virus DNA are Rapidly Corrected by Genomic Recombination Free

Abstract

The nonanucleotide motif TAATATTAC occurs in the intergenic region of all geminiviruses that have been examined to date. The motif is invariably located within the loop of a potential stem-loop structure that has been implicated in viral DNA replication. To investigate the contribution of these sequences to virus proliferation, African cassava mosaic virus (ACMV) DNA B mutants have been screened for their ability to infect when co-inoculated with DNA A. Mutants in which the putative stem structure was altered by the introduction of single nucleotide mismatches remained as infectious as the wild-type virus and the mutations were retained in the progeny. Mutants containing nucleotide substitutions within the loop sequences were similarly infectious but analysis of progeny showed that in most cases wild-type sequences were restored by recombination with DNA A. Stem-loop deletion mutants of both genomic components were not infectious when co-inoculated, although they were once again efficiently rescued by recombination when inoculated with the wild-type components. Co-inoculation of genomic components containing the motif TAGTAT- TAC did not result in a systemic infection while mutants containing the motif TAATATAC were infectious and the mutation was stable. The results demonstrate that ACMV will tolerate some modification to this highly conserved region of the genome that might allow more precise mapping of the position at which the viral DNA is nicked during replication.

  • Received:
  • Accepted:
  • Published Online:
© The Journal of General Virology 1994
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-11-3203
1994-11-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/11/JV0750113203.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-11-3203&mimeType=html&fmt=ahah

References

  1. Abouzid A. M., Polston J. E., Hiebert E. 1992; The nucleotide sequence of tomato mottle virus, a new geminivirus isolated from tomatoes in Florida. Journal of General Virology 73:3225–3229
     [Google Scholar]
  2. Andersen M. T., Richardson K. A., Harbison S. -A., Morris B. A. M. 1988; Nucleotide sequence of the geminivirus chloris striate mosaic virus. Virology 164:443–449
     [Google Scholar]
  3. Baas P. D., Jansz H. S. 1988; Single-stranded DNA phage origins. Current Topics in Microbiology and Immunology 136:31–69
     [Google Scholar]
  4. Briddon R. W., Lunness P., Chamberlin L. C. L., Pinner M. S., Brundish H., Markham P. G. 1992; The nucleotide sequence of an infectious insect-transmissible clone of the geminivirus Panicum streak virus. Journal of General Virology 73:1041–1047
     [Google Scholar]
  5. Chatani M., Matsumoto Y., Mizuta H., Ikegami M., Boulton M. I., Davies J. W. 1991; The nucleotide sequence and genome structure of the geminivirus miscanthus streak virus. Journal of General Virology 72:2325–2331
     [Google Scholar]
  6. Coutts R. H. A., Coffin R. S., Roberts E. J. F., Hamilton W. D. O. 1991; The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus. Journal of General Virology 72:1515–1520
     [Google Scholar]
  7. Curtis M. D., James R. 1991; Investigation of the specificity of the interaction between colicin E9 and its immunity protein by site- directed mutagenesis. Molecular Microbiology 5:2727–2733
     [Google Scholar]
  8. Donson J., Accotto G. P., Boulton M. I., Mullineaux P. M., Davies J. W. 1987; The nucleotide sequence of a geminivirus from Digitaria sanguinalis . Virology 161:160–169
     [Google Scholar]
  9. Dry I.B., Rigden J. E., Krake L. R., Mullineaux P. M., Rezaian M. A. 1993; Nucleotide sequence and genome organization of tomato leaf curl geminivirus. Journal of General Virology 74:147–151
     [Google Scholar]
  10. Etessami P., Watts J., Stanley J. 1989; Size reversion of African cassava mosaic virus coat protein gene deletion mutants during infection of Nicotiana benthamiana . Journal of General Virology 70:277–289
     [Google Scholar]
  11. Etessami P., Saunders K., Watts J., Stanley J. 1991; Mutational analysis of complementary-sense genes of African cassava mosaic virus DNA A. Journal of General Virology 72:1005–1012
     [Google Scholar]
  12. Fontes E. P. B., Luckow V. A., Hanley-Bowdoin L. 1992; A geminivirus replication protein is a sequence specific DNA binding protein. Plant Cell 4:597–608
     [Google Scholar]
  13. Frischmuth T., Zimmat G., Jeske H. 1990; The nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178:461–468
     [Google Scholar]
  14. Gardiner W. E., Sunter G., Brand L., Elmer J. S., Rogers S. G., Bisaro D. M. 1988; Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread or symptom development. EMBO Journal 7:899–904
     [Google Scholar]
  15. Gilbertson R. L., Faria J. C., Ahlquist P., Maxwell D. P. 1993; Genetic diversity in geminiviruses causing bean golden mosaic disease: the nucleotide sequence of the infectious cloned DNA components of a Brazilian isolate of bean golden mosaic geminivirus. Phytopathology 83:709–715
     [Google Scholar]
  16. Gruss A., Ehrlich S. D. 1989; The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiological Reviews 53:231–241
     [Google Scholar]
  17. Hamilton W. D. O., Stein V. E., Coutts R. H. A., Buck K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
     [Google Scholar]
  18. Harding R. M., Burns T. M., Hafner G., Dietzgen R. G., Dale J. L. 1993; Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. Journal of General Virology 74:323–328
     [Google Scholar]
  19. Heyraud F., Matzeit V., Kammann M., Schaefer S., Schell J., Gronenborn B. 1993; Identification of the initiation sequence for viral-strand DNA synthesis of wheat dwarf virus. EMBO Journal 12:4445–4452
     [Google Scholar]
  20. Hidayat S. H., Gilbertson R. L., Hanson S. F., Morales F. J., Ahlquist P., Russell D. R., Maxwell D. P. 1993; Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus. Phytopathology 83:181–187
     [Google Scholar]
  21. Hong Y. G., Robinson D. J., Harrison B. D. 1993; Nucleotide sequence evidence for the occurrence of three distinct whitefly- transmitted geminiviruses in cassava. Journal of General Virology 74:2437–2443
     [Google Scholar]
  22. Howarth A. J., Caton J., Bossert M., Goodman R. M. 1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proceedings of the National Academy of Sciences, U.S.A 82:3572–3576
     [Google Scholar]
  23. Klinkenberg F. A., Stanley J. 1990; Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana . Journal of General Virology 71:1409–1412
     [Google Scholar]
  24. Klinkenberg F. A., Ellwood S., Stanley J. 1989; Fate of African cassava mosaic virus coat protein deletion mutants after agroinoculation. Journal of General Virology 70:1837–1844
     [Google Scholar]
  25. Lazarowitz S. G., Lazdins I. B. 1991; Infectivity and complete nucleotide sequence of the cloned genomic components of a bipartite squash leaf curl geminivirus with a broad host range phenotype. Virology 180:58–69
     [Google Scholar]
  26. Lazarowitz S. G., Wu L. C., Rogers S. G., Elmer J. S. 1992; Sequence-specific interaction with the viral AL1 protein identifies a geminivirus DNA replication origin. Plant Cell 4:799–809
     [Google Scholar]
  27. MacDowell S. W., MacDonald H., Hamilton W. D. O., Coutts R. H. A., Buck K. W. 1985; The nucleotide sequence of cloned wheat dwarf virus DNA. EMBO Journal 4:2173–2180
     [Google Scholar]
  28. Michel B., Ehrlich S. D. 1986a; Illegitimate recombination at the replication origin of bacteriophage M13. Proceedings of the National Academy of Sciences, U.S.A 83:3386–3390
     [Google Scholar]
  29. Michel B., Ehrlich S. D. 1986b; Illegitimate recombination occurs between the replication origin of the plasmid pC194 and a progressing replication fork. EMBO Journal 5:3691–3696
     [Google Scholar]
  30. Mullineaux P. M., Donson J., Morris-Krsinich B. A. M., Boulton M. I., Davies J. W. 1984; The nucleotide sequence of maize streak virus DNA. EMBO Journal 3:3063–3068
     [Google Scholar]
  31. Navot N., Pichersky E., Zeidan M., Zamir D., Czosnek H. 1991; Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic component. Virology 185:151–161
     [Google Scholar]
  32. Revington G. N., Sunter G., Bisaro D. M. 1989; DNA sequences essential for replication of the B genome component of tomato golden mosaic virus. Plant Cell 1:985–992
     [Google Scholar]
  33. Rohde W., Randles J. W., Langridge P., Hanold D. 1990; Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176:648–651
     [Google Scholar]
  34. Saunders K., Lucy A., Stanley J. 1991; DNA forms of the geminivirus African cassava mosaic virus consistent with a rolling circle mechanism of replication. Nucleic Acids Research 19:2325–2330
     [Google Scholar]
  35. Schneider M., Jarchow E., Hohn B. 1992; Mutational analysis of the ‘conserved region’ of maize streak virus suggests its involvement in replication. Plant Molecular Biology 19:601–610
     [Google Scholar]
  36. Stanley J. 1983; Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature; London: 305643–645
     [Google Scholar]
  37. Stanley J., Gay M. R. 1983; Nucleotide sequence of cassava latent virus DNA. Nature; London: 301260–262
     [Google Scholar]
  38. Stanley J., Markham P. G., Callis R. J., Pinner M. S. 1986; The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5:1761–1767
     [Google Scholar]
  39. Stenger D. C., Revington G. N., Stevenson M. C., Bisaro D. M. 1991; Replicational release of geminivirus genomes from tandemly repeated copies: evidence for rolling circle replication of a plant viral DNA. Proceedings of the National Academy of Sciences, U.S.A 88:8029–8033
     [Google Scholar]
  40. Torres-Pacheco I., Garzon-Tiznado J. A., Herrera-Estrella L., Rivera-Bustamante R. F. 1993; Complete nucleotide sequence of pepper huasteco virus: analysis and comparison with bipartite geminiviruses. Journal of General Virology 74:2225–2231
     [Google Scholar]
  41. Townsend R., Stanley J., Curson S. J., Short M. N. 1985; Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein. EMBO Journal 4:33–37
     [Google Scholar]
  42. Townsend R., Watts J., Stanley J. 1986; Synthesis of viral DNA forms in Nicotiana plumbaginifolia protoplasts inoculated with cassava latent virus (CLV); evidence for the independent replication of one component of the CLV genome. Nucleic Acids Research 14:1253–1265
     [Google Scholar]
  43. Von Arnim A., Frischmuth T., Stanley J. 1993; Detection and possible functions of African cassava mosaic virus DNA B gene products. Virology 192:264–272
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-11-3203
Loading
Lethal Mutations Within the Conserved Stem-loop of African Cassava Mosaic Virus DNA are Rapidly Corrected by Genomic Recombination
J. Gen. Virol. 75, 3203 (1994); https://doi.org/10.1099/0022-1317-75-11-3203
/content/journal/jgv/10.1099/0022-1317-75-11-3203
/content/journal/jgv/10.1099/0022-1317-75-11-3203
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error