1887

Abstract

Feline herpesvirus type 1 (FHV-1) mutants were constructed, carrying a -galactosidase marker gene integrated into the region downstream of the gene encoding the homologue of glycoprotein C (gC) of herpes simplex virus type 1. In cell culture, no differences in replication were observed between mutants and the parent FHV-1 strain. However, in experimentally infected cats, mutants caused fewer clinical signs after oronasal administration although they replicated to the same extent as the parental strain. Sequence analysis in the region of the U segment surrounding the insertion site revealed an open reading frame (ORF 2) encoding a putative polypeptide of 2IK. RNA analysis indicated a corresponding transcript of 0·8 kb that was detected late after infection of cells in culture. This particular U locus downstream of the gC gene has not been thoroughly investigated in any of the herpesviruses. The putative gene product showed only limited evolutionary conservation since similarity could be found only with the assumed homologue of equine herpesvirus type 1. Further characterization of this newly identified FHV-1 gene involved in virulence may provide insight into the development of disease owing to herpesvirus infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-11-3107
1994-11-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/11/JV0750113107.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-11-3107&mimeType=html&fmt=ahah

References

  1. Allen G. P., Coogle L. D. 1988; Characterization of an equine herpesvirus type 1 gene encoding a glycoprotein (gpl3) with homology to herpes simplex virus glycoprotein C. Journal of Virology 62:2850–2858
    [Google Scholar]
  2. Bairoch A. 1991; PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Research 19:2241–2245
    [Google Scholar]
  3. Brandt C. R., Kintner R. L., Pumfery A. M., Visalli R. J., Grau D. R. 1991; The herpes simplex virus ribonucleotide reductase is required for ocular virulence. Journal of General Virology 72:2043–2049
    [Google Scholar]
  4. Cameron J. M., McDougall I., Marsden H. S., Preston V. G., Ryan D. M., Subak-Sharpe J. H. 1988; Ribonucleotide reductase encoded by herpes simplex virus is a determinant of the pathogenicity of the virus in mice and a valid antiviral target. Journal of General Virology 69:2607–2612
    [Google Scholar]
  5. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  6. Chou J., Kern E. R., Whitley R. J., Roizman B. 1990; Mapping of herpes simplex virus-1 neurovirulence to γ134·5, a gene non-essential for growth in culture. Science 250:1262–1266
    [Google Scholar]
  7. Cole G. E., Stacy-Phipps S., Nunberg J. H. 1990; Recombinant feline herpesviruses expressing feline leukemia virus envelope and gag proteins. Journal of Virology 64:4930–4938
    [Google Scholar]
  8. Crandell R. A., Fabricant C. G., Nelson-Rees W. A. 1973; Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9:176–185
    [Google Scholar]
  9. Fargeaud D., Benoit Jeannin C., Kato F., Chappuis G. 1984; Biochemical study of the feline herpesvirus 1. Identification of glycoproteins by affinity. Archives of Virology 80:69–82
    [Google Scholar]
  10. Field H. J., Wildy P. 1978; The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. Journal of Hygiene 81:267–277
    [Google Scholar]
  11. Frink R. J., Eisenberg R., Cohen G., Wagner E. K. 1983; Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein C. Journal of Virology 45:634–647
    [Google Scholar]
  12. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  13. Grail A., Harbour D. A., Chia W. 1991; Restriction endonuclease mapping of the genome of feline herpesvirus type 1. Archives of Virology 116:209–220
    [Google Scholar]
  14. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  15. Herrmann S. C., Gaskell R. M., Ehlers B., Ludwig H. 1984; Characterization of the feline herpesvirus genome and molecular epidemiology of isolates from natural outbreaks and latent infections. In Latent Herpesvirus Infections in Veterinary Medicine pp. 321–336 Wittmann G., Gaskell R. M., Rziha H. J. Edited by Boston: Martinus Nijhoff;
    [Google Scholar]
  16. Jacobson J. G., Leib D. A., Goldstein D. J., Bogard C. L., Schaffer P. A., Weller S. K., Coen D. M. 1989; A herpes simplex virus ribonucleotide reductase deletion mutant is defective for productive acute and reactivatable latent infections of mice and for replication in mouse cells. Virology 173:276–283
    [Google Scholar]
  17. Kimman T. G., De Wind N., Oei-Lie N., Pol J. M. A., Berns A. J. M., Gielkens A. L. J. 1992; Contribution of single genes within the unique short region of Aujeszky’s disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. Journal of General Virology 73:243–251
    [Google Scholar]
  18. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441
    [Google Scholar]
  19. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., McNab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  20. McKnight J. L. C., Pellett P. E., Jenkins F. J., Roizman B. 1987; Characterization and nucleotide sequence of two herpes simplex virus 1 genes whose products modulate α-trans-inducing factor-dependent activation of α genes. Journal of Virology 61:992–1001
    [Google Scholar]
  21. MacLean A. R., Ul-Fareed M., Robertson L., Harland J., Brown S. M. 1991; Herpes simplex virus type 1 deletion variants 1714 and 1716 pinpoint neurovirulence-related sequences in Glasgow strain 17+ between immediate early gene 1 and the ‘a’ sequence. Journal of General Virology 72:631–639
    [Google Scholar]
  22. Maes R. K., Fritsch S. L., Herr L., Rota P. A. 1984; Immunogenic proteins of feline rhinotracheitis virus. Journal of Virology 51:259–262
    [Google Scholar]
  23. Needleman S. B., Wunsch C. D. 1970; A general method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology 48:443–453
    [Google Scholar]
  24. Nunberg J. H., Wright D. K., Cole G. E., Petrovskis E. A., Post L. E., Compton T., Gilbert J. H. 1989; Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolate herpes virus gene homologs. Journal of Virology 63:3240–3249
    [Google Scholar]
  25. Pyles R. B., Sawtell N. M., Thompson R. L. 1992; Herpes simplex virus type 1 dUTPase mutants are attenuated for neuro-virulence, neuroinvasiveness, and reactivation from latency. Journal of Virology 66:6706–6713
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Sondermeijer P. J. A., Claessens J. A. J., Jenniskens P. E., Mockett A. P. A., Thijssen R. A. J., Willemse M. J., Morgan R. W. 1993; Avian herpesvirus as a live viral vector for the expression of heterologous antigens. Vaccine 11:349–358
    [Google Scholar]
  28. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  29. Telford E. A. R., Watson M. S., McBride K., Davison A. J. 1992; The DNA sequence of equine herpesvirus type 1. Virology 189:304–316
    [Google Scholar]
  30. Visalli R. J., Brandt C. R. 1991; The HSV-1 UL45 gene product is not required for growth in Vero cells. Virology 185:419–423
    [Google Scholar]
  31. Visalli R. J., Brandt C. R. 1993; The HSV-1 UL45 18 kDa gene product is a true late protein and a component of the virion. Virus Research 29:167–178
    [Google Scholar]
  32. Wardley R. C., Berlinski P. J., Thomsen D. R., Meyer A. L., Post L. E. 1992; The use of feline herpesvirus and baculovirus as vaccine vectors for the gag and env genes of feline leukaemia virus. Journal of General Virology 73:1811–1818
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-75-11-3107
Loading
/content/journal/jgv/10.1099/0022-1317-75-11-3107
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error