1887

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Volume 75, Issue 11

An Element Binding a C/EBP-related Transcription Factor Contributes to Negative Regulation of the Bovine Papillomavirus Type 4 Long Control Region Free

Abstract

Deletion of the NR2 element of the long control region (LCR) of bovine papillomavirus type 4 (BPV-4) was observed previously to lead to a fivefold increase in enhancer activity of a subfragment of the LCR. Further characterization of this element indicates that mutations in NR2 lead to increased enhancer activity in both mouse CT3 fibroblasts and in a transformed bovine epithelial cell line derived from an alimentary canal papilloma/m carcinoma, but not in primary bovine keratinocytes. Since similar oligonucleotide-nuclear factor complexes were obtained in electrophoretic mobility shift assays (EMSA) for all three cell types, the observed difference in negative activity may result from variation in the NR2-binding factor itself between primary and established/transformed cell lines, or from the involvement of other factors that vary between the lines. Characterization of the NR2-binding factor by heat stability and antibody supershifts in EMSA indicate that the factor is related to the CCAAT/enhancer- binding protein (C/EBP) family, and that one component of the complexes may be C/EBP/.

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© The Journal of General Virology 1994
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1994-11-01
2024-04-25
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An Element Binding a C/EBP-related Transcription Factor Contributes to Negative Regulation of the Bovine Papillomavirus Type 4 Long Control Region
J. Gen. Virol. 75, 3047 (1994); https://doi.org/10.1099/0022-1317-75-11-3047
/content/journal/jgv/10.1099/0022-1317-75-11-3047
/content/journal/jgv/10.1099/0022-1317-75-11-3047
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