1887

Abstract

Deletion of the NR2 element of the long control region (LCR) of bovine papillomavirus type 4 (BPV-4) was observed previously to lead to a fivefold increase in enhancer activity of a subfragment of the LCR. Further characterization of this element indicates that mutations in NR2 lead to increased enhancer activity in both mouse CT3 fibroblasts and in a transformed bovine epithelial cell line derived from an alimentary canal papilloma/m carcinoma, but not in primary bovine keratinocytes. Since similar oligonucleotide-nuclear factor complexes were obtained in electrophoretic mobility shift assays (EMSA) for all three cell types, the observed difference in negative activity may result from variation in the NR2-binding factor itself between primary and established/transformed cell lines, or from the involvement of other factors that vary between the lines. Characterization of the NR2-binding factor by heat stability and antibody supershifts in EMSA indicate that the factor is related to the CCAAT/enhancer- binding protein (C/EBP) family, and that one component of the complexes may be C/EBP/.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-11-3047
1994-11-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/11/JV0750113047.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-11-3047&mimeType=html&fmt=ahah

References

  1. Akira S., Isshiki H., Sugita T., Tanabe O., Kinoshita S., Nishio Y., Nakajima T., Hirano T., Kishimoto T. 1990; A nuclear factor for IL-6 expression (NF-1L6) is a member of a C/EBP family. EMBO Journal 9:1897–1906
    [Google Scholar]
  2. Bauknecht T., Angel P., Royer H. D., Zur Hausen H. 1992; Identification of a negative regulatory domain in the human papillomavirus type 18 promoter: interaction with the transcriptional repressor YY1. EMBO Journal II, 4607-4617
    [Google Scholar]
  3. Campo M. S., Jarrett W. F. H. 1986; Papillomavirus infection in cattle: viral and chemical cofactors in naturally occurring and experimentally induced tumours. CIBA Foundation Symposium 120:117–130
    [Google Scholar]
  4. Cao Z., Umek R. M., Mcknight S. L. 1991; Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes and Development 5:1538–1552
    [Google Scholar]
  5. Chang C. -J., Chen T. -T., Lei H. -Y., Chen D. -S., Lee S. -C. 1990; Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family. Molecular and Cellular Biology 10:6642–6653
    [Google Scholar]
  6. Cooper C., Johnson D., Roman C., Avitahl N., Tucker P., Calame K. 1992; The C/EBP family of transcriptional activators is functionally important for Ig VH promoter activity in vivo and in vitro. Journal of Immunology 149:3225–3231
    [Google Scholar]
  7. Cuthill S., Sibbet G. J., Campo M. S. 1993; Characterisation of a nuclear factor, papillomavirus enhancer binding protein factor-1, that binds to the long control region of human papillomavirus type 16 and contributes to enhancer activity. Molecular Carcinogenesis 8:96–104
    [Google Scholar]
  8. Descombes P., Schibler U. 1991; A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569–579
    [Google Scholar]
  9. Descombes P., Chojlier M., Lichtsteiner S., Falvey E., Schibler U. 1990; LAP, a novel member of the C/EBP gene family, encodes a liver-enriched transcriptional activator protein. Genes and Development 4:1541–1551
    [Google Scholar]
  10. Fuchs P. G., Pfister H. 1990; Papillomaviruses in epidermodysplasia verruciformis. Papillomavirus Report 1: 5 1–4
    [Google Scholar]
  11. Glass C. K., Holloway J. M., Devary O. V., Rosenfeld M. G. 1988; The thyroid hormone receptor binds with opposite transcriptional effects to a common sequence motif in thyroid hormone and estrogen response elements. Cell 54:313–323
    [Google Scholar]
  12. Gorman C. 1985; High efficiency gene transfer into mammalian cells. In DNA Cloning: A Practical Approach 2 pp 143–190 Glover D. M. Edited by Oxford & Washington D.C.: IRL Press;
    [Google Scholar]
  13. Hoppe-Seyler F., Butz K., Zur Hausen H. 1991; Repression of the human papillomavirus type 18 enhancer by the cellular transcription factor Oct-1. Journal of Virology 65:5613–5618
    [Google Scholar]
  14. Jackson M. E., Campo M. S. 1991; Positive and negative E2- independent regulatory elements in the long control region of bovine papillomavirus type 4. Journal of General Virology 72:877–883
    [Google Scholar]
  15. Jaggar R. T., Pennie W. D., Smith K. T., Jackson M. E., Campo M. S. 1990; Cooperation between bovine papillomavirus type 4 and ras in the morphological transformation of primary bovine fibroblasts. Journal of General Virology 71:3041–3046
    [Google Scholar]
  16. Jiang C. -K., Connolly D., Blumenberg M. 1991; Comparison of methods for transfection of human epidermal keratinocytes. Journal of Investigative Dermatology 97:969–973
    [Google Scholar]
  17. Kyo S., Inoue M., Nishio Y., Nakanishi K., Akira S., Inoue H., Yutsudo M., Tanizawa O., Hakura A. 1993; NF-IL6 represses early gene expression of human papillomavirus type 16 through binding to the noncoding region. Journal of Virology 61:1058–1066
    [Google Scholar]
  18. Landschulz W. H., Johnson P. F., Adashi E. Y., Graves B. J., Mcknight S. L. 1988; Isolation of a recombinant copy of the gene encoding C/EBP. Genes and Development 2:786–800
    [Google Scholar]
  19. Lopez-Cabrera M., Letovsky J., Hu K. -Q., Siddiqui A. 1990; Multiple liver-specific factors bind to the hepatitis B virus core/ pregenomic promoter: transactivation and repression by CCAAT/ enhancer binding protein. Proceedings of the National Academy of Sciences U.S.A: 875069–5073
    [Google Scholar]
  20. Mahoney C. W., Shuman J., Mcknight S. L., Chen H. -C., Huang K. -P. 1992; Phosphorylation of CCAAT-enhancer binding protein by protein kinase C attenuates site-selective DNA binding. Journal of Biological Chemistry 267:19396–19403
    [Google Scholar]
  21. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  22. May M., Dong X. P., Beyer-Finkler E., Stubenrauch F., Fuchs P. G., Pfister H. 1994; The E6/E7 promoter of extrachromosomal HPV16 DNA in cervical cancers escapes from cellular repression by mutation of target sequences for YY1. EM BO Journal 13:1460–1466
    [Google Scholar]
  23. Ossipow V., Descombes P., Schibler U. 1993; CCAAT/enhancer- binding protein mRNA is translated into multiple proteins with different transcription activation potentials. Proceedings of the National Academy of Sciences U.S.A: 908219–8233
    [Google Scholar]
  24. Pei D., Shih C. 1990; Transcriptional activation and repression by cellular DNA binding protein C/EBP. Journal of Virology 64:1517–1522
    [Google Scholar]
  25. Pennie W. D., Grindlay G. J., Cairney M., Campo M. S. 1993; Analysis of the transforming functions of bovine papillomavirus type 4. Virology 193:614–620
    [Google Scholar]
  26. Peterson C. L., Calame K. L. 1987; Complex protein binding within the mouse immunoglobulin heavy-chain enhancer. Molecular and Cellular Biology 7:4194–4203
    [Google Scholar]
  27. Poli V., Mancini F. P., Cortese R. 1990; IL-6DBP, a nuclear protein involved in interleukin-6 signal transduction, defines a new family of leucine zipper proteins related to C/EBP. Cell 63:643–653
    [Google Scholar]
  28. Reh H., Pfister H. 1990; Human papillomavirus type 8 contains cis-active positive and negative transcriptional control sequences. Journal of General Virology 71:2457–2462
    [Google Scholar]
  29. Roman C., Platero J. S., Shuman J., Calame K. 1990; Ig/EBP- 1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to C/EBP and heterodimerises with C/EBP. Genes and Development 4:1404–1415
    [Google Scholar]
  30. Ron D., Habener J. F. 1992; CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant negative inhibitor of gene transcription. Genes and Development 6:439–453
    [Google Scholar]
  31. Rosl F., Achtstatter T., Bauknecht T., Hutter K. -J., Futter-Man G., Zur Hausen H. 1991; Extinction of the HPV18 upstream regulatory region in cervical carcinoma cells after fusion with non-tumorigenic human keratinocytes under non-selective conditions. EMBO Journal 10:1337–1345
    [Google Scholar]
  32. Ryden T. A., Beemon K. 1989; Avian retroviral long terminal repeats bind CCAAT/enhancer-binding protein. Molecular and Cellular Biology 9:1155–1164
    [Google Scholar]
  33. Ryden T. A., De Mars M., Beemon K. 1993; Mutation of the C/EBP binding sites in the Rous sarcoma virus long terminal repeat and gag enhancers. Journal of Virology 67:2862–2870
    [Google Scholar]
  34. Sambrook J., Fritsch E. F., Maniatis T. 1989; . Molecular Cloning: A Laboratory Manual,, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Scott L. M., Civin C. I., Rorth P., Friedman A. D. 1992; A novel temporal expression pattern of three C/EBP family members in differentiating myelomonocytic cells. Blood 80:1725–1735
    [Google Scholar]
  36. Sears R. C., Sealy L. 1992; Characterisation of nuclear proteins that bind the EFII enhancer sequence in the Rous sarcoma virus long terminal repeat. Journal of Virology 66:6338–6352
    [Google Scholar]
  37. Smits H. L., Raadsheer E., Rood I., Mehendale S., Slater R. M., Van Der Noordaa J., Ter Schegget J. 1988; Induction of anchorage-independent growth of human embryonic fibroblasts with a deletion in the short arm of chromosome 11 by human papillomavirus type 16 DNA. Journal of Virology 62:4538–4543
    [Google Scholar]
  38. Smits P. H. M., De Ronde A., Smits H. L., Minnaar R. P., Van Der Noordaa J., Ter Schegget J. 1992a; Modulation of the human papillomavirus type 16 induced transformation and transcription by deletion of loci on the short arm of human chromosome 11 can be mimicked by SV40 small t. Virology 190:40–44
    [Google Scholar]
  39. Smits P. H. M., Smits H. L., Minnaar R. P., Hemmings B. A., Mayer-Jaekel R. E., Schuurman R., Van Der Noordaa J., Ter Schegget J. 1992b; The 55 kDa regulatory subunit of protein phosphatase 2A plays a role in the activation of the HPV16 long control region in human cells with a deletion in the short arm of chromosome 11. EMBO Journal 11:4601–4606
    [Google Scholar]
  40. Spalholz B. A., Yang Y. C., Howley P. M. 1985; Transactivation of a bovine papillomavirus transcriptional regulatory element by the E2 gene product. Cell 42:183–191
    [Google Scholar]
  41. Stubenrauch F., Malejczyk J., Fuchs P. G., Pfister H. 1992; Late promoter of human papillomavirus type 8 and its regulation. Journal of Virology 66:3485–3493
    [Google Scholar]
  42. Trautwein C., Caelles C., Van Der Geer P., Hunter T., Karin M., Chojkier M. 1993; Transactivation by NF-IL6/LAP is enhanced by phosphorylation of its activation domain. Nature; London: 364544–547
    [Google Scholar]
  43. Umek R. M., Friedman A. D., Mcknight S. L. 1991; CCAAT- enhancer binding protein: a component of a differentiation switch. Science 251:288–292
    [Google Scholar]
  44. Vallejo M., Ron D., Miller C. P., Habener J. F. 1993; C/ATF, a member of the activating transcription factor family of DNA binding proteins, dimerises with CAAT/enhancer-binding proteins and directs their binding to cAMP response elements. Proceedings of the National Academy of Sciences U.S.A: 904679–4683
    [Google Scholar]
  45. Vande Pol S. B., Howley P. M. 1990; A bovine papillomavirus constitutive enhancer is negatively regulated by the E2 repressor through competitive binding for a cellular factor. Journal of Virology 64:5420–5429
    [Google Scholar]
  46. Williams S. C., Cantwell C. A., Johnson P. F. 1991; A family of C/EBP-related proteins capable of forming covalently linked dimers in vitro. Genes and Development 5:1553–1567
    [Google Scholar]
  47. Wu T. C., Mounts P. 1988; Transcriptional regulatory elements in the noncoding region of human papillomavirus type 6. Journal of Virology 62:4722–4729
    [Google Scholar]
  48. Yasumoto S., Taniguchi A., Sohma K. 1991; Epidermal growth factor (EGF) elicits down-regulation of human papillomavirus type 16 (HPV-16) E6/E7 mRNA at the transcriptional level in an EGF- stimulated human keratinocyte cell line: functional role of EGF- responsive silencer in the HPV-16 long control region. Journal of Virology 65:2000–2009
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-11-3047
Loading
/content/journal/jgv/10.1099/0022-1317-75-11-3047
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error