1887

Abstract

We investigated the effects of pseudorabies virus (PRV), herpes simplex virus type 1 (HSV-1), and equineherpesvirus type 1 (EHV-1) ICP4 homologues on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression. This was done by using the transient expression chloramphenicol acetyltransferase (CAT) assay in Crandell feline kidney (CRFK) and whole fetus 4 cells transfected with a chimeric FIV LTR-CAT reporter construct in combination with effector plasmids expressing the PRV, HSV-1 or EHV-1 ICP4 homologue. The experiments demonstrated that the ICP4 homologues could significantly inhibit FIV LTR-directed gene expression. Moreover, the ICP4 homologues also exhibited a marked inhibitory effect on FIV replication in CRFK cells cotransfected with an infectious molecular clone of FIV.

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/content/journal/jgv/10.1099/0022-1317-75-10-2783
1994-10-01
2019-10-13
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-10-2783
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