1887

Journal of General Virology header logo

Volume 75, Issue 10

Comparative studies of the structural proteins and glycoproteins of equine herpesviruses 2 and 5 Free

Abstract

The structural proteins of equine herpesvirus 2 (EHV-2) and EHV-5, recently shown to be gammaherpesviruses, were identified and compared. Labelled proteins and glycoproteins were separated by SDS-PAGE and although EHV-2 and EHV-5 had similar protein profiles, bands in some positions were virus-specific. Six glycoproteins, with distinct profiles, were identified for both EHV-2 and EHV-5. Rabbit antisera to EHV-2 and EHV- 5 and horse antiserum to EHV-2 were used in radio- immunoprecipitations, Western blot analysis and ELISA to investigate the immunogenicity and cross-reactivity of virus proteins. These analyses revealed that while EHV-2 and EHV-5 proteins share many common epitopes, they also possess type-specific epitopes. A 0·71 kb region of the EHV-2 glycoprotein B (gB) gene was expressed as a fusion protein in . Antiserum raised in a rabbit to the EHV-2 fusion protein was used to identify a 64K EHV-2 protein as EHV-2 gB. Antiserum to EHV-2 gB was used to identify a 66K EHV-5 protein as EHV-5 gB. These proteins, which may represent subunits of gB rather than the entire molecule, appear the most immunodominant of the structural virion proteins as identified by Western blot.

  • Received:
  • Accepted:
  • Published Online:
© The Journal of General Virology 1994
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-10-2707
1994-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/10/JV0750102707.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-10-2707&mimeType=html&fmt=ahah

References

  1. Agius C. T., Nagesha H. S., Studdert M. J. 1992; Equine herpesvirus 5: comparisons with EHV2 (equine cytomegalovirus), cloning and mapping of a new equine herpesvirus with a novel genome structure. Virology 191:176–186
     [Google Scholar]
  2. Beall J. A., Mitchell G. F. 1986; Identification of a particular antigen from a parasite cDNA library using antibodies affinity purified from selected portions of Western blots. Journal of Immunological Methods 86:217–223
     [Google Scholar]
  3. Belak S., Palfi V., Tuboly S., Bartha L. 1980; Passive immunization of foals to prevent respiratory disease caused by equine herpesvirus type 2. Zentralblatt für Veterinärmedizin Reihe B 27:826–830
     [Google Scholar]
  4. Blakeslee J. R. Jr Olsen R. G., Mcallister E. S., Fassbender J., Dennis R. 1975; Evidence of respiratory tract infection induced by equine herpesvirus, type 2, in the horse. Canadian Journal of Microbiology 21:1940–1946
     [Google Scholar]
  5. Browning G. F., Studdert M. J. 1987; Genomic heterogeneity of equine betaherpesviruses. Journal of General Virology 68:1441–1447
     [Google Scholar]
  6. Browning G. F., Studdert M. J. 1989a; Physical mapping of a genome of equine herpesvirus 2 (equine cytomegalovirus). Archives of Virology 104:77–86
     [Google Scholar]
  7. Browning G. F., Studdert M. J. 1989b; Physical mapping of the genomic heterogeneity of isolates of equine herpesvirus 2 (equine cytomegalovirus). Archives of Virology 104:87–94
     [Google Scholar]
  8. Caughman G. B., Staczek J., O’Callaghan D. J. 1984; Equine cytomegalovirus: structural proteins of virions and nucleocapsids. Virology 134:184–195
     [Google Scholar]
  9. Costa R. H., Devi B. G., Anderson K. P., Gaylord B. H., Wagner E. K. 1981; Characterization of a major late herpes simplex virus type 1 mRNA. Journal of Virology 38:483–496
     [Google Scholar]
  10. Crabb B. S., Studdert M. J. 1990; Comparative studies of the proteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3: antibody response of the natural hosts. Journal of General Virology 71:2033–2041
     [Google Scholar]
  11. Crabb B. S., Studdert M. J. 1993; Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host. Journal of Virology 67:6332–6338
     [Google Scholar]
  12. Crabb B. S., Allen G. P., Studdert M. J. 1991; Characterization of the major glycoproteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3 using monoclonal antibodies. Journal of General Virology 72:2075–2082
     [Google Scholar]
  13. Crabb B. S., Nagesha H. S., Studdert M. J. 1992; Identification of equine herpesvirus 4 glycoprotein G: a type-specific, secreted glycoprotein. Virology 190:143–154
     [Google Scholar]
  14. Gibson W. 1983; Protein counterparts of human and simian cytomegaloviruses. Virology 128:391–406
     [Google Scholar]
  15. Gleeson L. J., Studdert M. J. 1977; Equine herpesviruses.Experimental infection of a foetus with type 2. Australian Veterinary Journal 53:360–362
     [Google Scholar]
  16. Gong M., Kieff E. 1990; Intracellular trafficking of two major Epstein-Barr virus glycoproteins, gp350/220 and gp110. Journal of Virology 64:1507–1516
     [Google Scholar]
  17. Gong M., Ooka T., Matsuo T., Kieff E. 1987; Epstein-Barr virus glycoprotein homologous to herpes simplex virus gB. Journal of Virology 61:499–508
     [Google Scholar]
  18. Harden T. J., Bagust T. J., Pascoe R. R., Spradbrow P. B. 1974; Studies on equine herpesviruses. 5. Isolation and characterisation of slowly cytopathic equine herpesviruses in Queensland. Australian Veterinary Journal 50:483–488
     [Google Scholar]
  19. Jolly P. D., Tu Z. F., Robinson A. J. 1986; Viruses associated with respiratory disease of horses in New Zealand: an update. New Zealand Veterinary Journal 34:46–50
     [Google Scholar]
  20. Karpas A. 1966; Characterization of a new herpes-like virus isolated from foal kidney. Annals de l’Institut Pasteur, Paris 110:688–696
     [Google Scholar]
  21. Kemeny L., Pearson J. E. 1970; Isolation of herpesvirus from equine leukocytes: comparison with equine rhinopneumonitis virus. Canadian Journal of Comparative Medicine 34:59–65
     [Google Scholar]
  22. Kono Y., Kobayashi K. 1964; Cytopathogenic equine orphan (CEO) virus in horse kidney cell culture. I. Isolation and properties. National Institute of Animal Health Quarterly, Japan 4:10–20
     [Google Scholar]
  23. Palfi V., Belak S., Molnar T. 1978; Isolation of equine herpesvirus type 2 from foals, showing respiratory symptoms. Zentralblatt für Veterinärmedizin Reihe B 25:165–167
     [Google Scholar]
  24. Pellett P. E., Biggin M. D., Barrell B., Roizman B. 1985; Epstein-Barr virus genome may encode a protein showing significant amino acid and predicted secondary structure homology with glycoprotein B of herpes simplex virus 1. Journal of Virology 56:807–813
     [Google Scholar]
  25. Plummer G., Waterson A. P. 1963; Equine herpesviruses. Virology 19:412–416
     [Google Scholar]
  26. Plummer G., Bowling C. P., Goodheart C. R. 1969; Comparison of four horse herpesviruses. Journal of Virology 4:738–741
     [Google Scholar]
  27. Plummer G., Goodheart C. R., Studdert M. J. 1973; Equine herpesviruses: antigenic relationships and deoxyribonucleic acid densities. Infection and Immunity 8:621–627
     [Google Scholar]
  28. Roeder P. L., Scott G. R. 1975; The prevalence of equid herpes virus 2 infections. Veterinary Record 96:404–405
     [Google Scholar]
  29. Roizman B., Desrosiers R. C., Fleckenstein B., Lopez C., Minson A. C., Studdert M. J. 1992; The family herpesviridae: an update. Archives of Virology 123:425–449
     [Google Scholar]
  30. Studdert M. J. 1971; Equine herpesviruses. 4. Concurrent infection in horses with strangles and conjunctivitis. Australian Veterinary Journal 47:434–436
     [Google Scholar]
  31. Studdert M. J., Turner A. J., Peterson J. E. 1970; Equine herpesviruses. 1. Isolation and characterisation of equine rhino-pneumonitis virus and other equine herpesviruses from horses. Australian Veterinary Journal 46:83–89
     [Google Scholar]
  32. Sugiura T., Fukuzawa Y., Kamada M., Ando Y., Hirasawa K. 1983; Isolation of equine herpesvirus type 2 from foals with pneumonitis. Bulletin of the Equine Research Institute 20:148–153
     [Google Scholar]
  33. Telford E. A. R., Studdert M. J., Agius C. T., Watson M. S., Aird H. C., Davison A. J. 1993; Equine herpesviruses 2 and 5 are γ-herpesviruses. Virology 195:492–499
     [Google Scholar]
  34. Turner A. J., Studdert M. J. 1970; Equine herpesviruses. 3. Isolation and epizootiology of slowly cytopathic viruses and the serological incidence of equine rhinopneumonitis. Australian Veterinary Journal 46:581–586
     [Google Scholar]
  35. Turner A. J., Studdert M. J., Peterson J. E. 1970; Equine herpesviruses 2. Persistence of equine herpesviruses in experimentally infected horses and in the experimental induction of abortion. Australian Veterinary Journal 46:90–98
     [Google Scholar]
  36. Vroman B., Luka J., Rodriguez M., Pearson G. R. 1985; Characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid of Epstein-Barr virus. Journal of Virology 53:107–113
     [Google Scholar]
  37. Welch H. M., Bridges C. G., Lyon A. M., Griffiths L., Edington N. 1992; Latent equidherpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and cocultivation from lymphoid tissues. Journal of General Virology 73:261–268
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-10-2707
Loading
Comparative studies of the structural proteins and glycoproteins of equine herpesviruses 2 and 5
J. Gen. Virol. 75, 2707 (1994); https://doi.org/10.1099/0022-1317-75-10-2707
/content/journal/jgv/10.1099/0022-1317-75-10-2707
/content/journal/jgv/10.1099/0022-1317-75-10-2707
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error