1887

Abstract

Rabies persists in Ontario wildlife in two predominant species: the red fox () and the striped skunk (). A protocol applying reverse transcription/polymerase chain reaction (RT/PCR) and restriction endonuclease analysis (REA) to the rabies virus nucleoprotein gene was previously reported by Nadin-Davis ( 74, 829–837, 1993) to be useful for discrimination of rabies virus variants in Ontario. Four main types, which showed no host species specificity but which did exhibit different geographical distributions, were identified.

Between 1989 and 1992 an area north and west of the city of North Bay experienced unusual and substantial rabies activity. In this report we describe the use of these molecular techniques to investigate the epidemiology of this recent rabies outbreak in central Ontario. It is shown that two of the four previously identified variants had invaded this region from the south and east, but in addition viruses very closely related to arctic isolates of rabies virus were found. The nucleoprotein and glycoprotein genes of this arctic type were sequenced and compared to those of its more southerly neighbours.

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1994-10-01
2022-05-16
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References

  1. Anilionis A., Wunner W. H., Curtis P. J. 1981; Structure of the glycoprotein gene in rabies virus. Nature; London: 294275–278
    [Google Scholar]
  2. Anon. 1992; First bait drop in northern Ontario. Rabies Reporter 3:1–2
    [Google Scholar]
  3. Benmansour A., Brahimi M., Tuffereau C., Coulon P., Lafay F., Flamand A. 1992; Rapid sequence evolution of street rabies glycoprotein is related to the highly heterogeneous nature of the viral population. Virology 187:33–45
    [Google Scholar]
  4. Bourhy H., Kissi B., Tordo N. 1993; Molecular diversity of the lyssavirus genus. Virology 194:70–81
    [Google Scholar]
  5. Charlton K. M. 1988; The pathogenesis of rabies. In Rabies pp 101–150 Campbell J. B., Charlton K. M. Edited by Boston: Kluwer Academic Publishers;
    [Google Scholar]
  6. Charlton K. M., Webster W. A., Casey G. A., Rupprecht C. E. 1988; Skunk rabies. Reviews of Infectious Diseases 10:S626–S628
    [Google Scholar]
  7. Conzelmann K. K., Cox J. H., Schneider L. G., Thiel H. J. 1990; Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19. Virology 175:485–499
    [Google Scholar]
  8. Johnston D. H., Fong D. W. 1992; Epidemiology of Arctic fox rabies. In Wildlife Rabies Control pp 45–49 Bogel K., Meslin F. X., Kaplan M. Edited by Royal Tunbridge Wells: Wells Medical Ltd;
    [Google Scholar]
  9. Kamolvarin N., Tirawatnpong T., Rattanasiwamoke R., Tirawatnpong S., Panpanich T., Hemachudha T. 1993; Diagnosis of rabies by polymerase chain reaction with nested primers. Journal of Infectious Diseases 167:207–210
    [Google Scholar]
  10. Kwok S., Sninsky J. J. 1989; Application of PCR to the detection of human infectious diseases. In PCR Technology, Principles and Applications for DNA Amplification pp 235–244 Erlich H. A. Edited by New York: Stockton Press;
    [Google Scholar]
  11. Nadin-Davis S. A., Casey G. A., Wandeler A. 1993; Identification of regional variants of the rabies virus within the Canadian province of Ontario. Journal of General Virology 74:829–837
    [Google Scholar]
  12. Sacramento D., Badrane H., Bourhy H., Tordo N. 1992; Molecular epidemiology of rabies virus in France: comparison with vaccine strains. Journal of General Virology 73:1149–1158
    [Google Scholar]
  13. Sacramento D., Bourhy H., Tordo N. 1991; PCR technique as an alternative method for diagnosis and molecular epidemiology of rabies virus. Molecular and Cellular Probes 5:229–240
    [Google Scholar]
  14. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer- directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Smith J. S., Fishbein D. B., Rupprecht C. E., Clark K. 1991; Unexplained rabies in three immigrants in the United States. New England Journal of Medicine 324:205–211
    [Google Scholar]
  17. Smith J. S., Orciari L. A., Yagar P. A., Seidel H. D., Warner C. K. 1992; Epidemiologic and historical relationships among 87 rabies virus isolates as determined by limited sequence analysis. Journal of Infectious Diseases 166:296–307
    [Google Scholar]
  18. Steck F., Wandeler A. 1980; The epidemiology of fox rabies in Europe. Epidemiologic Reviews 2:71–96
    [Google Scholar]
  19. Tabel H., Corner A. H., Webster W. A., Casey G. A. 1974; History and epizootiology of rabies in Canada. Canadian Veterinary Journal 15:271–281
    [Google Scholar]
  20. Tordo N., Poch O., Ermine A., Keith G., Rougeon F. 1986; Walking along the rabies genome: is the large G-L intergenic region a remnant gene?. Proceedings of the National Academy of Sciences U.S.A.: 833914–3918
    [Google Scholar]
  21. Tordo N., Poch O., Ermine A., Keith G., Rougeon F. 1988; Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses. Virology 165:565–576
    [Google Scholar]
  22. Tuffereau C., Leblois H., Benejean J., Coulon P., Lafay F., Flamand A. 1989; Arginine or lysine in position 333 of ERA and CVS glycoprotein is necessary for rabies virulence in adult mice. Virology 172:206–212
    [Google Scholar]
  23. Wandeler A. I. 1987; Virus infections of non-domestic carnivores: rabies virus. In Virus Infections of Carnivores pp 449–461 Appel M. J. Edited by Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  24. Webster W. A., Casey G. A., Charlton K. M., Wiktor T. J. 1985; Antigenic variants of rabies virus in isolates from Eastern, Central and Northern Canada. Canadian Journal of Comparative Medicine 49:186–188
    [Google Scholar]
  25. Webster W. A., Casey G. A. 1988; Diagnosis of rabies infection. In Rabies pp 201–222 Campbell J. B., Charlton K. M. Edited by Boston: Kluwer Academic Publishers;
    [Google Scholar]
  26. Wunner W. H. 1991; The chemical composition and molecular structure of rabies viruses. In The Natural History of Rabies pp 31–67 Baer G. M. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  27. Yelverton E., Norton S., Obijeski J. F., Goeddel D. V. 1983; Rabies virus glycoprotein analogs: biosynthesis in Escherichia coli. Science 219:614–620
    [Google Scholar]
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