RT Journal Article SR Electronic(1) A1 Minarovits, J. A1 Hu, L.-F. A1 Imai, S. A1 Harabuchi, Y. A1 Kataura, A. A1 Minarovits-Kormuta, S. A1 Osato, T. A1 Klein, G.YR 1994 T1 Clonality, expression and methylation patterns of the Epstein-Barr virus genomes in lethal midline granulomas classified as peripheral angiocentric T cell lymphomas JF Journal of General Virology, VO 75 IS 1 SP 77 OP 84 DO https://doi.org/10.1099/0022-1317-75-1-77 PB Microbiology Society, SN 1465-2099, AB We analysed the terminal repeats of Epstein-Barr virus (EBV) in DNAs isolated from six lethal midline granuloma (LMG) biopsies. A single fused terminal fragment could be detected in each case, indicating that these angiocentric peripheral T cell lymphomas represent clonal proliferations of cells infected with EBV on a single occasion. Using reverse transcriptase-PCR, we detected EBV nuclear antigen (EBNA) 1 and latent membrane protein (LMP) 1, but not EBNA 2 messages in LMG biopsy RNAs. The splicing pattern of the EBNA 1 message was consistent with the usage of a promoter localized in the BamHI F fragment (F promoter). The BamHI W fragment repeats and LMP-coding sequences were highly methylated in all cases. In contrast, the LMP regulatory sequences were found to be hypomethylated or partially methylated, as in LMP-expressing nasopharyngeal carcinomas., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-1-77