@article{mbs:/content/journal/jgv/10.1099/0022-1317-75-1-243, author = "Ryabov, E. V. and Generozov, E. V. and Kendall, T. L. and Lommel, S. A. and Zavriev, S. K.", title = "Nucleotide sequence of carnation ringspot dianthovirus RNA-1", journal= "Journal of General Virology", year = "1994", volume = "75", number = "1", pages = "243-247", doi = "https://doi.org/10.1099/0022-1317-75-1-243", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-1-243", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The nucleotide sequence of carnation ringspot virus (CRSV) RNA-1, the type member of the dianthovirus genus, has been determined. The 3756 nucleotide genomic RNA-1 contains three large open reading frames (ORFs), capable of encoding 27K, 54K and 38K polypeptides. In addition, a small ORF encoding a 10K polypeptide at the 3′ terminus of the RNA has been identified. The gene organization of CRSV RNA-1 is similar to those of red clover necrotic mosaic (RCNMV) and sweet clover necrotic mosaic (SCNMV) diantho- viruses with the exception that CRSV RNA-1 contains the additional 3′-terminal ORF. The 27K and 54K proteins possess significant sequence similarity to corresponding polypeptides of the other dianthoviruses. The 54K protein also contains the conserved RNA- dependent RNA polymerase motif. The identification of a shifty heptanucleotide preceding the p27 ORF termination codon and a predicted secondary structure following the terminator suggest that a translational frameshifting event allows translation to continue past the p27 ORF into the p54 ORF, which is in the − 1 frame, generating an 88K fusion protein. Amino acid sequence alignment of the 38K protein with the corresponding RCNMV and SCNMV polypeptides indicate that this is the viral capsid protein.", }