1887

Abstract

The segmented double-stranded RNA genome of reovirus (NLRV) was cloned, and the nucleotide sequence of genome segment S10 and terminal nucleotide sequences of the rest of the segments were determined. Genome segment S10 of NLRV consisted of 1430 nucleotides with a single open reading frame extending for 1293 nucleotides from nucleotide 46. It encoded a polypeptide of 431 amino acids with an of 49·4K, which was a non-structural protein. The plus-strand RNA of all genome segments had the same conserved trinucleotide 5′AGU- and hexanucleotide- GUUGUC 3′in the 5′ - and 3′ -terminal regions, respectively. These conserved terminal sequences resembled those found in the segments of the members of the genus

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1994-01-01
2024-04-19
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References

  1. Antczak J. B., Chmelo R., Pickup D. J., Joklik W. K. 1982; Sequences at both termini of the 10 genes of reovirus serotype 3 (strain dearing). Virology 121:307–319
    [Google Scholar]
  2. Anzola J. V., Xu Z., Asamizu T., Nuss D. L. 1987; Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences, U.S.A 84:8301–8305
    [Google Scholar]
  3. Azuhata F., Uyeda I., Shikata E. 1992; Conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus. Journal of General Virology 73:1593–1595
    [Google Scholar]
  4. Both G. W., Bellamy A. R., Siegman L. J. 1984; Nucleotide sequence of the dsRNA genomic segment 7 of simian 11 rotavirus. Nucleic Acids Research 12:1621–1626
    [Google Scholar]
  5. Cashdollar L. W., Esparza J., Hudson G. R., Shmelo R., Lee P. W. K., Joklik W. K. 1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U.S.A 79:7644–7648
    [Google Scholar]
  6. Chowdhury K. 1991; One step ‘miniprep’ method for the isolation of plasmid DNA. Nucleic Acids Research 19:2792
    [Google Scholar]
  7. Elleman T. C., Hoyne P. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. 1983; Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus. Nucleic Acids Research 11:4689–4701
    [Google Scholar]
  8. Fossiez F., Belloncik S., Arella M. 1989; Nucleotide sequence of the polyhedrin gene of Euxoa scandens cytoplasmic polyhedrosis virus (EsCPV). Virology 169:462–465
    [Google Scholar]
  9. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and nomenclature of viruses. Archives of Virology suppl. 2
    [Google Scholar]
  10. Holmes I. H., Boccardo G., Estes M. K., Furuichi M. K., Hoshino Y., Joklik W. K., Mccrae M., Mertens P. P. C., Milne R. G., Shikata E., Winton J. R. 1993; Family Reoviridae. In Classification and Nomenclature of Viruses. Archives of Virology suppl in press
    [Google Scholar]
  11. Imai M., Richardson M. A., Ikegami N., Shatkin A. J., Furuichi Y. 1983; Molecular cloning of double-stranded RNA virus genomes. Proceedings of the National Academy of Sciences, U.S.A 80:373–377
    [Google Scholar]
  12. Kuchino Y., Nishimura S., Smith R., Furuichi Y. 1982; Homologous terminal sequences in the double-stranded RNA genome segments of cytoplasmic polyhedrosis virus of the silkworm Bombyx mori. Journal of Virology 44:538–543
    [Google Scholar]
  13. Kudo H., Uyeda I., Shikata E. 1991; Viruses in the phytoreovirus genus of the Reoviridae family have the same conserved terminal sequences. Journal of General Virology 72:2857–2866
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  15. Mccrae M. A. 1981; Terminal structure of reovirus RNAs. Journal of General Virology 55:393–403
    [Google Scholar]
  16. Marzachi C., Boccardo G., Nuss D. L. 1991; Cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within a single genomic segment. Virology 180:518–526
    [Google Scholar]
  17. Mertens P. P. C., Sangar D. V. 1985; Analysis of the terminal sequences of the genome segments of four orbiviruses. Virology 140:55–67
    [Google Scholar]
  18. Moss S. R., Fukusho A., Nuttall P. A. 1990; RNA segment 5 of Broadhaven virus, a tick-borne orbivirus, shows sequence homology with segment 5 of bluetongue virus. Virology 179:482–484
    [Google Scholar]
  19. Nault L. R., Ammar E. D. 1989; Leafhopper and planthopper transmission of plant viruses. Annual Review of Entomology 34:503–529
    [Google Scholar]
  20. Noda H., Ishikawa K., Hibino H., Omura T. 1991a; A reovirus in the brown planthopper, Nilaparvata lugens. Journal of General Virology 72:2425–2430
    [Google Scholar]
  21. Noda H., Ishikawa K., Hibino H., Kato H., Omura T. 1991b; Nucleotide sequences of genome segments S8, encoding a capsid protein, and S10, encoding a 36K protein, of rice gall dwarf virus. Journal of General Virology 72:2837–2842
    [Google Scholar]
  22. Nuss D. L., Dall D. J. 1990; Structural and functional properties of plant reovirus genomes. Advances in Virus Research 38:249–306
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Sano H., Feix G. 1976; Terminal riboadenylate transferase from Escherichia coli, characterization and application. European Journal of Biochemistry 71:577–583
    [Google Scholar]
  25. Suzuki N., Watanabe Y., Kusano T., Kitagawa Y. 1990; Sequence analysis of rice dwarf phytoreovirus genome segments S4, S5, and S6: comparison with the equivalent wound tumor virus segments. Virology 179:446–454
    [Google Scholar]
  26. Van Regenmortel M. H. V. 1990; Virus species, a much overlooked but essential concept in virus classification. Intervirology 31:241–254
    [Google Scholar]
  27. Ward C. W., Elleman T. C., Azad A. A., Dyall-Smith M. L. 1984; Nucleotide sequence of gene segment 9 encoding a nonstructural protein of UK bovine rotavirus. Virology 134:249–253
    [Google Scholar]
  28. Yan J., Kudo H., Uyeda I., Lee S.-Y., Shikata E. 1992; Conserved terminal sequences of rice ragged stunt virus genomic RNA. Journal of General Virology 73:785–789
    [Google Scholar]
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