1887

Abstract

The sequence of the VP7 gene of human rotavirus strain HCR3 was determined and its predicted amino acid sequence was compared with that of other rotavirus strains. The VP7 gene is 1062 nucleotides long and contains a single open reading frame of 981 nucleotides capable of encoding a protein of 326 amino acids. The VP7 amino acid sequence similarity of strain HCR3 to those of various human and animal G3 serotypes ranged from 88·7 to 99·4%, and from 60·4 to 88·3 % to strains representing each of the other 13 G serotypes. Alignment of four variable regions [VR4, VR5(A), VR7(B) and VR8(C)] of HCR3 with those of G3 strains of different host species showed that HCR3 possesses a sequence almost identical to that of canine rotaviruses and feline rotavirus strain CAT97 in all four regions. A considerable divergence in regions VR4, VR7(B) and VR8(C) was found with strains of human, mouse, monkey, horse and rabbit rotaviruses. This observation together with results of our previous study on VP4 indicated that human rotavirus HCR3 is genetically more closely related to animal rotaviruses than to other human rotaviruses.

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1994-01-01
2022-08-13
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References

  1. Birch C. J., Heath R. L., Marshall J. A., Liu S., Gust I. D. 1985; Isolation of feline rotaviruses and their relationship to human and simian isolates by electropherotype and serotype. Journal of General Virology 66:2731–2735
    [Google Scholar]
  2. Browning G. F., Chalmers R. M., Fitzgerald T. A., Snodgrass D. R. 1991a; Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. Journal of General Virology 72:1059–1064
    [Google Scholar]
  3. Browning G. F., Fitzgerald T. A., Chalmers R. M., Snodgrass D. R. 1991b; A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate F123. Journal of Clinical Microbiology 29:2043–2046
    [Google Scholar]
  4. Coulson B., Kirkwood C. 1991; Relation of VP7 amino acid sequence to monoclonal antibody neutralization of rotavirus and rotavirus monotype. Journal of Virology 65:5968–5974
    [Google Scholar]
  5. Dyall-Smith M. L., Lazdins I., Tregar G. W., Holmes I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proceedings of the National Academy of Sciences, U.S. A 83:3465–3468
    [Google Scholar]
  6. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  7. Flores J., Myslinski J., Kalica A. R., Greenberg H. B., Wyatt R. G., Kapikian A. Z., Chanock R. M. 1982; In vitro transcription of two human rotaviruses. Journal of Virology 43:1032–1037
    [Google Scholar]
  8. Flores J., Hoshino Y., Boeggeman E., Purcell R., Chanock R. M., Kapikian A. Z. 1986; Genomic relatedness among animal rotaviruses. Archives of Virology 87:273–285
    [Google Scholar]
  9. Glass R. I., Keith J., Nakagomi O., Nakagomi T., Askaa J., Kapikian A. Z. 1985; Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology 141:292–298
    [Google Scholar]
  10. Gouvea V., Glass R. I., Woods P., Taniguchi K., Clark H. F., Forrester B., Fang Z.-Y. 1990; Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. Journal of Clinical Microbiology 28:276–282
    [Google Scholar]
  11. Gouvea V., Ramirez C., Li B., Santos N., Saif L., Clark H. F., Hoshino Y. 1993; Restriction endonuclease analysis of the vp7 genes of human and animal rotaviruses. Journal of Clinical Microbiology 31:917–923
    [Google Scholar]
  12. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. 1987; Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology 161:153–159
    [Google Scholar]
  13. Green K. Y., Hoshino Y., Ikegami N. 1989; Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology 168:429–433
    [Google Scholar]
  14. Greenberg H. B., Valdesuso J., Van Wyke K., Midthun K., Walsh M., Mcauliffe V., Wyatt R. G., Kalica A. R., Flores J., Hoshino Y. 1983; Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. Journal of Virology 47:267–275
    [Google Scholar]
  15. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. 1984; Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. Journal of Infectious Diseases 149:694–702
    [Google Scholar]
  16. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. 1985; Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences, U.S.A 82:8701–8704
    [Google Scholar]
  17. Kapikian A. Z., Chanock R. M. 1990; Rotaviruses. In Virology, 2nd edn. pp. 1353–1404 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  18. Li B., Clark H. F., Gouvea V. 1993; Nucleotide sequence of the VP4 encoding gene of an unusual human rotavirus (HCR3). Virology 196:825–830
    [Google Scholar]
  19. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. 1988; Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology 165:511–517
    [Google Scholar]
  20. Nakagomi O., Nakagomi T. 1991; Genetic diversity and similarity among mammalian rotaviruses in relation to interspecies transmission of rotavirus. Archives of Virology 120:43–55
    [Google Scholar]
  21. Nakagomi O., Oshima A., Aboudy A., Shif I., Mochizuki M., Nakagomi T., Gotlieb-Stmatsky T. 1990; Molecular identification by RNA-RNA hybridization of a human rotavirus that is closely related to rotaviruses of feline and canine origin. Journal of Clinical Microbiology 28:1198–1203
    [Google Scholar]
  22. Nakagomi T., Nakagomi O. 1989; RNA-RNA hybridization identifies a human rotavirus that is genetically related to feline rotavirus. Journal of Virology 63:1431–1434
    [Google Scholar]
  23. Nishikawa K., Hoshino Y., Taniguchi K., Green K. Y., Greenberg H. B., Kapikian A. Z., Chanock R. M., Gorziglia M. 1989; Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology 171:503–515
    [Google Scholar]
  24. Offit P. A., Blavat G. 1986; Identification of the two rotavirus genes determining neutralization specificities. Journal of Virology 57:376–378
    [Google Scholar]
  25. Offit P. A., Clark H. F., Plotkin S. A. 1983; Response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization. Infection and Immunity 42:293–300
    [Google Scholar]
  26. Taniguchi K., Urasawa T., Morita Y., Greenberg H. B., Urasawa S. 1987; Direct serotyping of rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4- specific monoclonal antibodies to VP7. Journal of Infectious Diseases 155:1159–1166
    [Google Scholar]
  27. Taniguchi K., Urasawa N., Kobayashi N., Gorziglia M., Urasawa S. 1990; Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup 1 specificity and long RNA pattern: implication for new G serotype specificity. Journal of Virology 64:5640–5644
    [Google Scholar]
  28. Xu L., Harbour D., Mccrae M. A. 1991; Sequence of the gene encoding the major neutralization antigen (VP7) of serotype 10 rotavirus. Journal of General Virology 72:177–180
    [Google Scholar]
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