%0 Journal Article %A Rapp, Maria %A Lučin, Pero %A Messerle, Martin %A Loh, Lambert C. %A Koszinowski, Ulrich H. %T Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus %D 1994 %J Journal of General Virology, %V 75 %N 1 %P 183-188 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-75-1-183 %I Microbiology Society, %X The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5·0 kb, which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity of the MCMV gH with a 87K envelope glycoprotein described previously by Loh and Qualtiere. Immunization of mice with the Vac-gH recombinant gave rise to an anti-gH serum, which neutralized MCMV without complement in vitro. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-1-183