1887

Abstract

We have isolated a panel of monoclonal antibodies that recognize the DNA-binding domain of the herpes simplex virus type 1 (HSV-1) immediate early polypeptide Vmw175. The mice used for the fusions had been immunized with the isolated Vmw175 DNA-binding domain. This had been purified from bacteria that carried a phage T7 expression plasmid with the DNA-binding domain coding region. The epitopes recognized by the monoclonal antibodies were mapped by using a family of truncated versions of the DNA-binding domain, which had also been expressed in the bacterial expression system. The monoclonal antibodies divided into at least four different groups according to this mapping. Several of the monoclonal antibodies recognized Vmw175 expressed in infected BHK cells by HSV-1 strain 17 in Western blots. One of them also recognized the corresponding protein of varicella-zoster virus gene 62. This is further illustration of the relatedness of the two polypeptides.

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/content/journal/jgv/10.1099/0022-1317-74-9-1955
1993-09-01
2021-10-20
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References

  1. Disney GH, Everett RD. 1990; A herpes simplex virus type 1 recombinant with both copies of the Vmwl75 coding sequences replaced by the homologous varicella-zoster virus open reading frame.. Journal of General Virology 71:2681–2689
    [Google Scholar]
  2. Everett RD, Elliott M, Hope G, Orr A. 1991; Purification of the DNA binding domain of herpes simplex virus type 1 immediate-early protein Vmwl75 as a homodimer and extensive mutagenesis of its DNA recognition site.. Nucleic Acids Research 19:4901–4908
    [Google Scholar]
  3. Littler E, Yeo J, Killington RA, Purifoy DJH, Powell KL. 1981; Antigenic and structural conservation of herpesvirus DNA-binding proteins.. Journal of General Virology 56:409–419
    [Google Scholar]
  4. McGeoch DJ, Dolan A, Donald S, Brauer DHK. 1986; Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1.. Nucleic Acids Research 14:1727–1764
    [Google Scholar]
  5. Paterson T, Everett RD. 1988; Mutational dissection of the HSV-1 immediate-early protein Vmwl75 involved in transcriptional transactivation and repression.. Virology 166:186–196
    [Google Scholar]
  6. Snowden BW, Kinchington PR, Powell KL, Halliburton IW. 1985; Antigenic and biochemical analysis of gB of herpes simplex virus types 1 and 2 and of cross-reacting glycoproteins induced by bovine mammillitis virus and equine herpesvirus type 1.. Journal of General Virology 66:231–247
    [Google Scholar]
  7. Tyler JK, Everett RD. 1993; The DNA binding domain of the varicella-zoster virus gene 62 protein interacts with multiple sequences which are similar to the binding site of the related protein of herpes simplex virus type 1.. Nucleic Acids Research 21:513–522
    [Google Scholar]
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