1887

Abstract

The complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus (ACLSV-A) was determined. The genome is 7552 nucleotides excluding the poly(A) tail and contains three open reading frames (ORFs 1, 2 and 3), encoding proteins with values of 216503 (216.5K), 50453 (50.4K) and 21394 (21.4K), respectively. Nucleotide sequence comparisons between ACLSV-A and the previously sequenced ACLSV from plum (ACLSV-P) showed that the sequence identity at the nucleotide level was 79.8%. Amino acid sequence identities of ORFs 1 and 2 between both isolates were 88.4% and 79.9%, respectively. The 21.4K protein encoded by ORF 3 of ACLSV-A had an amino acid sequence identity of 88.6% with the 28.3K protein encoded by ORF 3 of ACLSV-P. Immunoblot analysis of the 21.4K protein expressed in showed that this protein is the coat protein of ACLSV-A.

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/content/journal/jgv/10.1099/0022-1317-74-9-1927
1993-09-01
2019-11-19
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-9-1927
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