1887

Abstract

The complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus (ACLSV-A) was determined. The genome is 7552 nucleotides excluding the poly(A) tail and contains three open reading frames (ORFs 1, 2 and 3), encoding proteins with values of 216503 (216∙5K), 50453 (50∙4K) and 21394 (21∙4K), respectively. Nucleotide sequence comparisons between ACLSV-A and the previously sequenced ACLSV from plum (ACLSV-P) showed that the sequence identity at the nucleotide level was 79∙8%. Amino acid sequence identities of ORFs 1 and 2 between both isolates were 88∙4% and 79∙9%, respectively. The 21.4K protein encoded by ORF 3 of ACLSV-A had an amino acid sequence identity of 88∙6% with the 28∙3K protein encoded by ORF 3 of ACLSV-P. Immunoblot analysis of the 21∙4K protein expressed in showed that this protein is the coat protein of ACLSV-A.

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1993-09-01
2022-09-25
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