Sunn-hemp mosaic tobamovirus (SHMV) facilitated the spread of the cowpea strain of southern bean sobemovirus (SBMV-C) only in inoculated leaves of common bean (Phaseolus vulgaris L. cv. Bountiful), a resistant host for SBMV-C. Tissue prints of bean primary leaves doubly inoculated with SHMV and SBMV-C, developed by Western blotting, showed the presence of the SBMV-C capsid antigen in the mesophyll and epidermis, but no antigen was detected in the conducting bundles. Typical SBMV-C virions were not seen in electron micrographs of immunogold-labelled mesophyll cells; instead, specifically labelled, amorphous protein clumps were found in the vacuole. Particles of smaller diameter than that of typical SBMV-C virions were specifically trapped by SBMV antibodies following immunosorbent electron microscopy of extracts from doubly infected leaves. SBMV-C coat protein from infected Vigna unguiculata L. (cowpea) and bean plants showed no difference in its mobility following electrophoresis in denaturing SDS–polyacrylamide gels. Lack of efficient assembly of SBMV-C virions does not impede cell-to-cell movement of the virus in doubly infected leaves of bean, yet it is probably an important factor in determining the inability of SBMV-C to move into and/or through the vascular system of this host.
BerrymanMA,
RodewaldRD.
1990; An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes.. Journal of Histochemistry and Cytochemistry 38:159–170
BlakeMS,
JohnstonKH,
Russell-JonesGJ,
GotschlichEC.
1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.. Analytical Biochemistry 136:175–179
CassabGI,
VarnerJE.
1987; Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper.. Journal of Cell Biology 105:2581–2588
CitovskyV,
KnorrD,
SchusterG,
ZambryskiP.
1990; The P30 movement protein of tobacco mosaic virus is single strand nucleic acid binding protein.. Cell 60:637–647
CitovskyV,
WongML,
ShawAL,
PrasadBVV,
ZambryskiP.
1992; Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids.. Plant Cell 4:397–111
DawsonWO,
BubrickP,
GranthamGL.
1988; Modifications of tobacco mosaic virus coat protein gene affecting replication, movement and symptomatology.. Phytopathology 78:783–789
DingB,
HaudenshieldJS,
HullRI,
WolfS,
BeachyRN,
LucasWJ.
1992; Secondary plasmodesmata are specific sites of localization of the mosaic virus movement protein in transgenic tobacco plants.. Plant Cell 4:915–928
GardinerWE,
SunterG,
BrandL,
ElmerJS,
RogersSG,
BisaroDM.
1988; Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread or symptom development.. EMBO Journal 4:899–904
HattaT,
FranckiRIB.
1981; Identification of small polyhedral virus particles in thin sections of plant cells by an enzyme cytochemical technique.. Journal of Ultrastructure Research 74:116–129
HorisbergerM,
RosserJ.
1977; Colloidal gold, a useful marker for transmission and scanning electron microscopy.. Journal of Histochemistry and Cytochemistry 25:295–305
SiegelA,
ZaitlinM,
SehgalOP.
1962; The isolation of defective tobacco mosaic virus strains.. Proceedings of the National Academy of Sciences, U.S.A. 48:1845–1851
TremaineJH,
RonaldWP,
KellyEM.
1981; Comparison of highly basic cyanogen bromide peptides from strains of southern bean mosaic virus.. Canadian Journal of Microbiology 27:654–663
van LentJWM.
1988Localization of viral antigens in leaf protoplasts and plants by immunogold labelling. Ph.D. thesis: Wageningen Agricultural University;