1887

Abstract

Sunn-hemp mosaic tobamovirus (SHMV) facilitated the spread of the cowpea strain of southern bean sobemovirus (SBMV-C) only in inoculated leaves of common bean ( L. cv. Bountiful), a resistant host for SBMV-C. Tissue prints of bean primary leaves doubly inoculated with SHMV and SBMV-C, developed by Western blotting, showed the presence of the SBMV-C capsid antigen in the mesophyll and epidermis, but no antigen was detected in the conducting bundles. Typical SBMV-C virions were not seen in electron micrographs of immunogold-labelled mesophyll cells; instead, specifically labelled, amorphous protein clumps were found in the vacuole. Particles of smaller diameter than that of typical SBMV-C virions were specifically trapped by SBMV antibodies following immunosorbent electron microscopy of extracts from doubly infected leaves. SBMV-C coat protein from infected L. (cowpea) and bean plants showed no difference in its mobility following electrophoresis in denaturing SDS–polyacrylamide gels. Lack of efficient assembly of SBMV-C virions does not impede cell-to-cell movement of the virus in doubly infected leaves of bean, yet it is probably an important factor in determining the inability of SBMV-C to move into and/or through the vascular system of this host.

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1993-09-01
2021-10-18
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References

  1. Atabekov JG, Dorokhov YL. 1984; Plant virus-specific transport function and resistance of plants to viruses.. Advances in Virus Research 29:313–364
    [Google Scholar]
  2. Atabekov JG, Taliansky ME. 1990; Expression of a plant-virus coded transport function of different viral genomes.. Advances in Virus Research 38:201–248
    [Google Scholar]
  3. Berryman MA, Rodewald RD. 1990; An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes.. Journal of Histochemistry and Cytochemistry 38:159–170
    [Google Scholar]
  4. Blake MS, Johnston KH, Russell-Jones GJ, Gotschlich EC. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.. Analytical Biochemistry 136:175–179
    [Google Scholar]
  5. Caspar DLD, Klug A. 1962; Physical principles in the construction of regular viruses.. Cold Spring Harbor Symposia on Quantitative Biology 27:1–24
    [Google Scholar]
  6. Cassab GI, Varner JE. 1987; Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper.. Journal of Cell Biology 105:2581–2588
    [Google Scholar]
  7. Citovsky V, Zambryski P. 1991; How do plant virus nucleic acids move through intercellular connections?. Bio Essays 8:373–379
    [Google Scholar]
  8. Citovsky V, Knorr D, Schuster G, Zambryski P. 1990; The P30 movement protein of tobacco mosaic virus is single strand nucleic acid binding protein.. Cell 60:637–647
    [Google Scholar]
  9. Citovsky V, Wong ML, Shaw AL, Prasad BVV, Zambryski P. 1992; Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids.. Plant Cell 4:397–111
    [Google Scholar]
  10. Clark MF, Lister RM, Bar-Joseph M. 1986; ELISA techniques.. Methods in Enzymology 118:742–766
    [Google Scholar]
  11. Dawson WO, Hilf ME. 1992; Host range determinants of plant viruses.. Annual Review of Plant Physiology and Plant Molecular Biology 43:527–555
    [Google Scholar]
  12. Dawson WO, Bubrick P, Grantham GL. 1988; Modifications of tobacco mosaic virus coat protein gene affecting replication, movement and symptomatology.. Phytopathology 78:783–789
    [Google Scholar]
  13. Ding B, Haudenshield JS, Hull RI, Wolf S, Beachy RN, Lucas WJ. 1992; Secondary plasmodesmata are specific sites of localization of the mosaic virus movement protein in transgenic tobacco plants.. Plant Cell 4:915–928
    [Google Scholar]
  14. Frens G. 1973; Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions.. Nature, Physical Science 241:20–22
    [Google Scholar]
  15. Fuentes AL, Hamilton RI. 1991; Sunn-hemp mosaic virus facilitates cell-to-cell spread of southern bean mosaic virus in a nonpermissive host.. Phytopathology 81:1302–1305
    [Google Scholar]
  16. Gardiner WE, Sunter G, Brand L, Elmer JS, Rogers SG, Bisaro DM. 1988; Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread or symptom development.. EMBO Journal 4:899–904
    [Google Scholar]
  17. Gegenheimer P. 1990; Guide to protein purification.. Methods in Enzymology 182:174–193
    [Google Scholar]
  18. Hatta T, Francki RIB. 1981; Identification of small polyhedral virus particles in thin sections of plant cells by an enzyme cytochemical technique.. Journal of Ultrastructure Research 74:116–129
    [Google Scholar]
  19. Horisberger M, Rosser J. 1977; Colloidal gold, a useful marker for transmission and scanning electron microscopy.. Journal of Histochemistry and Cytochemistry 25:295–305
    [Google Scholar]
  20. Hull R. 1977; The stabilization of the particles of turnip rosette virus and of other members of the southern bean mosaic virus.. Virology 79:58–66
    [Google Scholar]
  21. Hull R. 1989; The movement of viruses in plants.. Annual Review of Phytopathology 24:213–240
    [Google Scholar]
  22. Kassanis B, Varma A. 1975; Sunn-hemp mosaic virus.. CMI/AAB. Descriptions of Plant Viruses no. 153
    [Google Scholar]
  23. Laemmli UK. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  24. Matthews REF. 1991 Plant Virology, 3rd edn.. San Diego: Academic Press;
    [Google Scholar]
  25. Petty ITD, Jackson AO. 1990; Mutational analysis of barley stripe mosaic virus RNA β.. Virology 179:712–718
    [Google Scholar]
  26. Robards AW, Lucas WJ. 1990; Plasmodesmata. Annual Review of Plant Physiology and Plant Molecular Biology 41:369–419
    [Google Scholar]
  27. Saito T, Yamanaka K, Okada Y. 1990; Long-distance movement and viral assembly of tobacco mosaic virus mutants.. Virology 62:329–336
    [Google Scholar]
  28. Siegel A, Zaitlin M, Sehgal OP. 1962; The isolation of defective tobacco mosaic virus strains.. Proceedings of the National Academy of Sciences, U.S.A. 48:1845–1851
    [Google Scholar]
  29. Slot JW, Geuze HJ. 1981; Sizing of Protein A-colloidal gold probes for immunoelectron microscopy.. Journal of Cell Biology 90:533–536
    [Google Scholar]
  30. Tremaine JH, Ronald WP, Kelly EM. 1981; Comparison of highly basic cyanogen bromide peptides from strains of southern bean mosaic virus.. Canadian Journal of Microbiology 27:654–663
    [Google Scholar]
  31. van Lent JWM. 1988 Localization of viral antigens in leaf protoplasts and plants by immunogold labelling. Ph.D. thesis: Wageningen Agricultural University;
    [Google Scholar]
  32. Zaitlin M, Hull R. 1987; Plant virus-host interactions.. Annual Review of Plant Physiology 38:291–315
    [Google Scholar]
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