1887

Journal of General Virology header logo

Volume 74, Issue 9

Changes in populations of cauliflower mosaic virus DNA and RNA forms during turnip callus proliferation Free

Abstract

Cauliflower mosaic virus (CaMV) nucleic acids accumulate in the cell in different structural conformations related to their roles in gene expression, replication and virion assembly. We have characterized changes in the population CaMV DNA and RNA replication products which occur following culture of infected turnip leaves under conditions where callus proliferates. After only 5 days in culture, a significant increase in the level of genome-length and subgenomic supercoiled (SC) DNA forms was observed by two-dimensional (2D) gel electrophoresis. Open circular (OC) molecules, corresponding to these SC DNAs, with mobilities consistent with the presence of a single break in each strand, were also detected after 5 days culture. By 10 days culture, the proportion of OC molecules with only one break per double-stranded molecule had increased. After 34 days culture, SC DNA with a range of sizes predominated in the unencapsidated DNA fraction. The change in pattern of OC and SC DNA forms during callus proliferation suggests a possible precursor/product relationship involving generation of deleted molecules from gap-containing virion DNA-like molecules followed by sequential repair of the gaps to produce SC DNA. Moreover, heterogeneity in the mobility of OC DNAs in the neutral dimension of 2D electrophoresis, a feature exhibited by twisted CaMV virion DNA, changed during the time-course suggesting that untwisting occurs during gap repair. Although the relative abundance of SC DNA increased during callus proliferation, CaMV polyadenylated 35S and 19S transcripts declined together with immediate reverse transcription products. We suggest that cellular changes during callus growth lead to a decline in authentic CaMV transcripts in the cytoplasm resulting in cessation of synthesis of viral products and progeny DNA genomes. In consequence, pre-existing virion DNAs return to the nucleus, possibly as a result of a relaxation in a cytoplasmic control mechanism, where they are assembled into various forms of SC DNA. The presence of CaMV SC DNAs in replicating cells might also enhance illegitimate integration into host chromosomes, as hybridization of CaMV DNA to high DNA was observed.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1993
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-9-1887
1993-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/9/JV0740091887.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-9-1887&mimeType=html&fmt=ahah

References

  1. Benfey PN, Chua NH. 1990; The cauliflower mosaic virus 35S promoter: combinatorial regulation of transcription in plants.. Science 250:959–966
     [Google Scholar]
  2. Covey SN. 1985; Organisation and expression of the cauliflower mosaic virus genome. In Molecular Plant Virology vol 2: pp 121–159Edited by Davies J. W. Boca Raton: CRC Press;
     [Google Scholar]
  3. Covey SN. 1991; Pathogenesis of a plant pararetrovirus: CaMV.. Seminars in Virology 2:151–159
     [Google Scholar]
  4. Covey SN, Turner DS. 1986; Hairpin DNAs of cauliflower mosaic virus generated by reverse transcription in vivo.. EMBO Journal 11:2763–2768
     [Google Scholar]
  5. Covey SN, Turner DS. 1991; Comparison of viral nucleic acid intermediates at early and late stages of cauliflower mosaic virus infection suggests a feedback regulatory mechanism.. Journal of General Virology 72:2603–2606
     [Google Scholar]
  6. Covey SN, Turner DS, Lucy AP, Saunders K. 1990; Host regulation of the cauliflower mosaic virus multiplication cycle.. Proceedings of the National Academy of Sciences, U.S.A. 87:1633–1637
     [Google Scholar]
  7. Donson J, Hull R. 1983; Physical mapping and molecular cloning of caulimovirus DNA.. Journal of General Virology 64:2281–2288
     [Google Scholar]
  8. Espinoza AM, Medina V, Hull R, Markham PG. 1991; Cauliflower mosaic virus gene II product forms distinct inclusion bodies in infected plant cells.. Virology 185:337–343
     [Google Scholar]
  9. Fuetterer J, Hohn T. 1991; Translation of a polycistronic mRNA in the presence of the cauliflower mosaic virus transactivator protein.. EMBO Journal 10:3887–3897
     [Google Scholar]
  10. Gardner RC, Shepherd RJ. 1980; A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA.. Virology 106:159–161
     [Google Scholar]
  11. Hohn T, Fuetterer J. 1992; Transcriptional and translational control of gene expression in cauliflower mosaic virus.. Current Opinion in Genetics and Development 2:90–96
     [Google Scholar]
  12. Hull R, Covey SN. 1983; Characterisation of cauliflower mosaic virus DNA forms isolated from infected turnip leaves.. Nucleic Acids Research 11:1881–1895
     [Google Scholar]
  13. Hull R, Howell SH. 1978; Structure of the cauliflower mosaic virus genome. II. Variation in DNA structure and sequence between isolates.. Virology 86:482–193
     [Google Scholar]
  14. Hull R, Shepherd RJ, Harveys JD. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles.. Journal of General Virology 31:93–100
     [Google Scholar]
  15. Leisner SM, Turgeon R, Howell SH. 1992; Long distance movement of cauliflower mosaic virus in infected turnip plants.. Molecular Plant-Microbe Interactions 5:41–17
     [Google Scholar]
  16. Linstead PJ, Hills GJ, Plaskitt KA, Wilson IG, Harker CL, Maule AJ. 1988; The subcellular location of the gene 1 product of cauliflower mosaic virus is consistent with a function associated with virus spread.. Journal of General Virology 69:1809–1818
     [Google Scholar]
  17. Olszewski N, Hagen G, Guilfoyle TJ. 1982; A transcriptionally active covalently closed minichromosome of cauliflower mosaic virus DNA isolated from infected turnip leaves.. Cell 29:395–402
     [Google Scholar]
  18. Rollo F, Covey SN. 1985; Cauliflower mosaic virus DNA persists as supercoiled forms in cultured turnip cells.. Journal of General Virology 66:603–608
     [Google Scholar]
  19. Sanfacjon H, Wieczorek A. 1992; Analysis of cauliflower mosaic virus RNAs in Brassica species showing a range of susceptibility to infection.. Virology 190:30–39
     [Google Scholar]
  20. Saunders K, Lucy AP, Covey SN. 1990; Susceptibility of Brassica species to cauliflower mosaic virus infection is related to a specific stage in the virus multiplication cycle.. Journal of General Virology 71:1641–1647
     [Google Scholar]
  21. Sherker AH, Marion PL. 1991; Hepadnavirus and hepatocellular carcinoma.. Annual Review of Microbiology 45:475–508
     [Google Scholar]
  22. Turner DS, Covey SN. 1988; Discontinuous hairpin DNAs synthesized in vivo following specific and non-specific priming of cauliflower mosaic virus DNA ( + ) strands.. Virus Research 9:49–62
     [Google Scholar]
  23. Turner DS, Covey SN. 1993; Reverse transcription products generated by defective plus-strand synthesis during cauliflower mosaic virus replication.. Virus Research 28:171–185
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-9-1887
Loading
Changes in populations of cauliflower mosaic virus DNA and RNA forms during turnip callus proliferation
J. Gen. Virol. 74, 1887 (1993); https://doi.org/10.1099/0022-1317-74-9-1887
/content/journal/jgv/10.1099/0022-1317-74-9-1887
/content/journal/jgv/10.1099/0022-1317-74-9-1887
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error