1887

Abstract

Rod-shaped flexuous viruses were partially purified from garlic plants () showing typical mosaic symptoms. The genome was shown to be composed of RNA with a poly(A) tail of an estimated size of 10 kb as shown by denaturing agarose gel electrophoresis. We constructed cDNA libraries and screened four independent clones, which were designated GV-A, GV-B, GV-C and GV-D, using Northern and Southern blot hybridization. Nucleotide sequence determination of the cDNAs, two of which correspond to nearly one-third of the virus genomic RNA, shows that all of these viruses possess an identical genomic structure and that also at least four proteins are encoded in the viral cDNA, their s being estimated to be 15K, 27K, 40K and 11K. The 15K open reading frame (ORF) encodes the core-like sequence of a zinc finger protein preceded by a cluster of basic amino acid residues. The 27K ORF probably encodes the viral coat protein (CP), based on both the existence of some conserved sequences observed in many other rod-shaped or flexuous virus CPs and an overall amino acid sequence similarity to potexvirus and carlavirus CPs. The 11K ORF shows significant amino acid sequence similarities to the corresponding 12K proteins of the potexviruses and carlaviruses. On the other hand, the 40K ORF product does not resemble any other plant virus gene products reported so far. The genomic organization in the 3ʹ region of the garlic viruses resembles, but clearly differs from, that of carlaviruses. Phylogenetic analysis based upon the amino acid sequence of the viral capsid protein also indicates that the garlic viruses have a unique and distinct domain different from those of the potexvirus and carlavirus groups. The results suggest that the garlic viruses described here belong to an unclassified and new virus group closely related to the carlaviruses.

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1993-09-01
2021-10-25
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References

  1. AbouHaidar MG, Lai R. 1989; Nucleotide sequence of the 3ʹ-terminal region of clover yellow mosaic virus RNA. Journal of General Virology 70:1871–1875
    [Google Scholar]
  2. Beck DL, Guilford PJ, Voot DM, Anderson MT, Forster RSL. 1991; Triple gene block proteins of white clover mosaic potexvirus are required for transport.. Virology 183:695–702
    [Google Scholar]
  3. Bundin VS, Vishnyakova OA, Zakharyev VM, Morozov SYu, Atabekov JG, Skryabin KG. 1986; Comparative studies of potexvirus genomes: homology between the primary structure of coat protein genes.. Doklady Akademii Nauk SSSR 290:728–733
    [Google Scholar]
  4. Bos L, FIuijberts N, FIuttinga H, Maat DZ. 1978a; Leek yellow stripe virus and its relationships to onion yellow dwarf virus; characterization, ecology and possible control.. Netherlands Journal of Plant Pathology 84:185–204
    [Google Scholar]
  5. Bos L, FIuttinga H, Maat DZ. 1978b; Shallot latent virus, a new carlavirus.. Netherlands Journal of Plant Pathology 84:227–237
    [Google Scholar]
  6. Cadilhac B, Quiot JB, Marrou J, Leroux JP. 1976; Mise en evidence au microscope electronique de deux virus diflerents infectant fail (Allium sativum L.) et l’echalote (Allium cepa L. var ascalonicum).. Annales de Phytopathologie 8:65–72
    [Google Scholar]
  7. Delecollb B, Lot FL. 1979; Virus disease of garlic: evolution of research and future prospects.. In Proceedings, 3rd Conference ISHS Working Group on Vegetable Viruses, Bari, Italy pp 83–84
    [Google Scholar]
  8. Dolja VV, Boyko VP, Agranovsky AA, Koonin EV. 1991; Phylogeny of capsid proteins of rod-shaped and filamentous RNA plant viruses: two families with distinct patterns of sequence and probably structure conservation.. Virology 184:79–86
    [Google Scholar]
  9. Forster RLS, Bevan MW, FFarbison SA, Gardner RC. 1988; The complete nucleotide sequence of the potexvirus white clover mosaic virus.. Nucleic Acids Research 16:291–303
    [Google Scholar]
  10. Foster GD, Millar AW, Meehan BM, Mills PR. 1990; Nucleotide sequence of the 3ʹ-terminal region of Helenium virus S RNA.. Journal of General Virology 71:1877–1880
    [Google Scholar]
  11. Gramstat A, Courtpozanis A, Rohde W. 1990; The 12 kDa protein of potato virus M displays properties of a nucleic acidbinding regulatory protein.. FEES Letters 276:34–38
    [Google Scholar]
  12. Hari V. 1981; The RNA of tobacco etch virus: further characterization and detection of protein linked to RNA.. Virology 112:391–399
    [Google Scholar]
  13. Haylor MTM, Brunt AA, Coutts RHA. 1990; Conservation of the 3ʹ terminal nucleotide sequence in five carlaviruses.. Nucleic Acids Research 18:6127
    [Google Scholar]
  14. Huisman MJ, Linthorst HJM, Bol JF, Cornelissen BJC. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses.. Journal of General Virology 69:1789–1798
    [Google Scholar]
  15. Koonin EV. 1991; The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses.. Journal of General Virology 72:2197–2206
    [Google Scholar]
  16. Lee YW, Yamazaki S, Osaki T, Inouye T. 1979; Two elongated viruses in garlic, garlic latent virus and garlic mosaic virus.. Annals of the Phytopathological Society of Japan 45:727–734
    [Google Scholar]
  17. Mackenzie DJ, Tremaine JH, Stace-Smith R. 1989; Organization and interviral homologies of the 3ʹ-terminal portion of potato virus S RNA.. Journal of General Virology 70:1053–1063
    [Google Scholar]
  18. Mohamed NA, Young BR. 1981; Garlic yellow streak virus, a potyvirus infecting garlic in New Zealand.. Annals of Applied Biology 97:65–74
    [Google Scholar]
  19. Morozov SYu, Dolja VV, Atabekov JG. 1989; Probable reassortment of genomic elements among elongated RNA-con-taining plant viruses.. Journal of Molecular Evolution 29:52–62
    [Google Scholar]
  20. Rupasov VV, Morozov SYu, Kanyuka KV, Zavriev SK. 1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences.. Journal of General Virology 70:1861–1869
    [Google Scholar]
  21. Sako I. 1989; Occurrence of garlic latent virus in Allium species.. Plant Protection (in Japanese) 43:389–392
    [Google Scholar]
  22. Sako N. 1976; Virus disease in garlic (I).. Annals of the Phytopathological Society of Japan 42:383
    [Google Scholar]
  23. Sako N. 1978; Virus disease in garlic (II).. Annals of the Phytopathological Society of Japan 43:114
    [Google Scholar]
  24. Sambrook J, Fritsch EF, Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  25. Sanger F, Nicklen S, Coulson AR. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  26. Sehnke PC, Mason AM, Hood SJ, Lister RM, Johnson JE. 1989; A ‘zinc-finger’-type binding domain in tobacco streak virus coat protein.. Virology 168:48–56
    [Google Scholar]
  27. Shukla DD, Ward CW. 1989; Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group.. Advances in Virus Research 36:273–314
    [Google Scholar]
  28. Sit TL, AbouHaidar MG, Holy S. 1989; Nucleotide sequence of papaya mosaic virus RNA.. Journal of General Virology 70:2325–2331
    [Google Scholar]
  29. Zuidema D, Linthorst HJM, Huisman MJ, Asjes CJ, Bol JF. 1989; Nucleotide sequence of narcissus mosaic virus RNA.. Journal of General Virology 70:267–276
    [Google Scholar]
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