1887

Abstract

We have produced monoclonal antibodies against two multicapsid nuclear polyhedrosis virus (OpMNPV) transcriptional trans-activators, IE-1 and IE-2. Temporal analysis of IE-1 and IE-2 proteins have shown that IE-1 continues to increase in steady-state levels from 0 to 120 h post-infection, whereas IE-2 declines by 36 h post-infection. At least five different electrophoretic forms of IE-1 are present in OpMNPV-infected LD652Y cells of which three appear to be from the non-spliced IE-1 gene. Cotransfection experiments also showed that IE-1 causes a reduction in the levels of IE-2 protein but concurrently IE-2 increases IE-1 expression. Western blot analysis indicated that a form of IE-1 copurified with budded virions but did not copurify with the polyhedron-derived virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-9-1819
1993-09-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/9/JV0740091819.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-9-1819&mimeType=html&fmt=ahah

References

  1. Batterson W, Roizman B. 1983; Characterization of the herpes simplex virion-associated factor responsible for the induction of a genes.. Journal of Virology 46:371–377
    [Google Scholar]
  2. Blissard GW, Rohrmann GF. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus.. Virology 170:537–555
    [Google Scholar]
  3. Blissard GW, Wenz JR. 1992; Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion.. Journal of Virology 66:6829–6835
    [Google Scholar]
  4. Bradford MB, Blissard GW, Rohrmann GF. 1990; Characterization of the infection cycle of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus in Lymantria dispar cells.. Journal of General Virology 71:2841–2846
    [Google Scholar]
  5. Campbell MEM, Palfreyman JW, Preston CM. 1984; Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription.. Journal of Molecular Biology 180:1–19
    [Google Scholar]
  6. Carson DD, Summers MD, Guarino LA. 1984; Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements.. Journal of Virology 65:945–951
    [Google Scholar]
  7. Chisholm GE, Henner DJ. 1988; Multiple early transcripts and splicing of the Autographa californica nuclear polyhedrosis virus IE-1 gene.. Journal of Virology 62:3193–3200
    [Google Scholar]
  8. Dalrymple MA, McGeoch DJ, Davison AJ, Preston CM. 1985; DNA sequence of the herpes simplex virus type 1 gene product responsible for transcriptional activation of immediate early promoters.. Nucleic Acids Research 13:7865–7879
    [Google Scholar]
  9. Friesen PD, Miller LK. 1987; Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the Hindlll K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus.. Journal of Virology 61:2264–2272
    [Google Scholar]
  10. Gombart AF, Pearson MN, Rohrmann GF, Beaudreau GS. 1989; A baculovirus polyhedral envelope-associated protein: genetic location, nucleotide sequence, and immunocytochemical characterization.. Virology 169:189–193
    [Google Scholar]
  11. Guarino LA, Summers MD. 1986; Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-early gene.. Journal of Virology 57:563–571
    [Google Scholar]
  12. Guarino LA, Summers MD. 1988; Functional mapping of Autographa californica nuclear polyhedrosis virus genes required for late gene expression.. Journal of Virology 62:463–171
    [Google Scholar]
  13. Harlow E, Lane D. 1988 New York: Cold Spring Harbor Laboratory.; Antibodies: A Laboratory Manual.
    [Google Scholar]
  14. Hohmann AW, Faulkner P. 1993; Monoclonal antibodies to baculovirus structural proteins: determination of specificities by Western blot analysis.. Virology 125:432–444
    [Google Scholar]
  15. Hunter T, Karin M. 1992; The regulation of transcription by phosphorylation.. Cell 70:375–387
    [Google Scholar]
  16. Kovacs GR, Guarino LA, Graham BL, Summers MD. 1991a; Identification of spliced baculovirus RNAs expressed late in infection.. Virology 185:633–643
    [Google Scholar]
  17. Kovacs GR, Guarino LA, Summers MD. 1991b; Novel regulatory properties of the IE1 and IE0 transactivators encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus.. Journal of Virology 65:5281–5288
    [Google Scholar]
  18. Kovacs GR, Choi J, Guarino LA, Summers MD. 1992; Functional dissection of the Autographa californica nuclear polyhedrosis virus immediate-early 1 transcriptional regulatory protein.. Journal of Virology 66:7429–7437
    [Google Scholar]
  19. Krappa R, Knebel-Morsdorf D. 1991; Identification of the very early transcribed baculovirus gene PE-38.. Journal of Virology 65:805–812
    [Google Scholar]
  20. Laemmli UK. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  21. Leisy DJ, Rohrmann GF, Beaudreau GS. 1984; Conservation of genome organization in two multicapsid nuclear polyhedrosis viruses.. Journal of Virology 52:699–702
    [Google Scholar]
  22. Lemaster S, Roizman B. 1980; Herpes simplex virus phospho-proteins: II. Characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion.. Journal of Virology 35:798–811
    [Google Scholar]
  23. Lu A, Carstens EC. 1992; Transcription analysis of the FcoRI D region of the baculovirus Autographa californica nuclear polyhedrosis virus identifies an early 4-kilobase RNA encoding the essential pl43 gene.. Journal of Virology 66:655–663
    [Google Scholar]
  24. Miller LK. 1988; Baculoviruses as gene expression vectors.. Annual Review of Microbiology 42:177–199
    [Google Scholar]
  25. Nissen MS, Friesen PD. 1989; Molecular analysis of the transcriptional regulatory region of an early baculovirus gene.. Journal of Virology 63:493–503
    [Google Scholar]
  26. OReilly DR, Passarelli AL, Goldman IF, Miller LK. 1990; Characterization of the DA26 gene in a hypervariable region of the Autographa californica nuclear polyhedrosis virus genome.. Journal of General Virology 71:1029–1037
    [Google Scholar]
  27. Pearson MN, Russell RLQ, Rohrmann GF, Beaudreau GS. 1988; p39, a major baculovirus structural protein: immunocytochemical characterization and genetic location.. Virology 167:407–413
    [Google Scholar]
  28. Pellett PE, McKnight JLC, Jenkins FJ, Roizman B. 1985; Nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus DNA fragment capable of trans-inducing a genes.. Proceedings of the National Academy of Sciences, U.S.A. 82:5870–5874
    [Google Scholar]
  29. Ptashne M. 1992; How eukaryotic transcriptional activators work.. Nature, London 335:683–685
    [Google Scholar]
  30. Quant-Russell RL, Pearson MN, Rohrmann GF, Beaudreau GS. 1987; Characterization of baculovirus plO synthesis using monoclonal antibodies.. Virology 160:9–19
    [Google Scholar]
  31. Rice GW, Miller LK. 1986; Baculovirus transcription in the presence of inhibitors and in non-permissive Drosophila cells.. Virus Research 6:155–172
    [Google Scholar]
  32. Sadowski I, Ma J, Triezenberg S, Ptashne M. 1988; Gal4-VP16 is an unusually potent transcriptional activator.. Nature, London 335:563–564
    [Google Scholar]
  33. Sadowski I, Niedbala D, Wood K, Ptashne M. 1991; GAL4 is phosphorylated as a consequence of transcriptional activation.. Proceedings of the National Academy of Sciences, U.S.A. 88:10510–10514
    [Google Scholar]
  34. Sambrook J, Fritsch E. F, Maniatis T. 1989, 2nd edn.. New York: Cold Spring Harbor Laboratory.;
  35. Smith DB, Johnson KS. 1988; Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione 5-transferase.. Gene 67:31–40
    [Google Scholar]
  36. Spear PG, Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.. Journal of Virology 9:143–159
    [Google Scholar]
  37. Summers MD, Smith GE. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures.. Texas Agricultural Experiment Station Bulletin no.1555
    [Google Scholar]
  38. Theilmann DA, Stewart S. 1991; Identification and characterization of the IE-1 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus.. Virology 180:492–508
    [Google Scholar]
  39. Theilmann DA, Stewart S. 1992a; Molecular analysis of the trans-activating IE-2 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus.. Virology 187:84–96
    [Google Scholar]
  40. Theilmann DA, Stewart S. 1992b; Tandemly repeated sequence at the 3′ end of the IE-2 gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element.. Virology 187:97–106
    [Google Scholar]
  41. Thiem SM, Miller LK. 1989; A baculovirus gene with a novel transcription pattern encodes a polypeptide with a zinc finger and a leucine zipper.. Journal of Virology 63:4489–4497
    [Google Scholar]
  42. Triezenberg SJ, Kingsbury RC, McKnight SL. 1988; Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression.. Genes and Development 2:718–729
    [Google Scholar]
  43. Wu X, Stewart S, Theilmann DA. 1993; Characterization of an early gene coding for a highly basic 8-9K protein from the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus.. Journal of General Virology 74:1591–1598
    [Google Scholar]
  44. Yao F, Courtney RJ. 1989; A major transcriptional regulatory protein (ICP4) of herpes simplex virus type 1 is associated with purified virions.. Journal of Virology 63:3338–3344
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-9-1819
Loading
/content/journal/jgv/10.1099/0022-1317-74-9-1819
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error