1887

Abstract

A small RNA virus with novel characteristics has been isolated from laboratory-bred larvae of . Infection by the stunt virus causes severe retardation of larval development and subsequent death. Its particles are isometric, 38 nm in diameter, and have a buoyant density of 1.296 g/ml in caesium chloride. The viral capsid has two major non-glycosylated protein components with s of 65000 and 6000, and contains a genome composed of two non-polyadenylated single-stranded RNA molecules with lengths of 2.4 kb and 5.5 kb. The 5′ termini of these RNAs are capped; their 3′ termini are unblocked. translations of the viral RNAs showed synthesis of large proteins of sizes near the maximum coding capacity of each strand along with synthesis of numerous smaller proteins; no evidence for processing of precursors was seen. The physicochemical properties of the virus are most similar to those of the Nudaurelia ω virus, a provisional member of the Tetraviridae, although no antigenic relationship was observed between the two viruses. The bipartite genome and distinct capsid structure of these two viruses indicate the existence of a previously unrecognized virus group.

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1993-09-01
2022-08-13
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References

  1. Adams JR. 1991; Introduction and classification of viruses of invertebrates.. Edited by Adams J. R., Bonami J. R. Atlas of Invertebrate Viruses Boca Raton: CRC Press.; pp In 1–8
    [Google Scholar]
  2. Adams JR, Guzo D. 1991; Unclassified viruses of insects.. Edited by Adams J. R., Bonami J. R. Atlas of Invertebrate Viruses Boca Raton: CRC Press.; pp In 553–592
    [Google Scholar]
  3. Agrawal DK, Johnson JE. 1992; Sequence and analysis of the capsid protein of Nudaurelia capensis co virus, an insect virus with T = 4 icosahedral symmetry.. Virology 190:804–806
    [Google Scholar]
  4. Cavarelli J, Bomu W, Liuas L, Kim S, Minor W, Munshi S, Muchmore S, Schmidt T, Hendry DA, Johnson J. E. 1991; Crystallization and preliminary structure analysis of an insect virus with T = 4 quasi-symmetry: Nudaurelia capensis co virus.. Acta crystallographica B47:23–29
    [Google Scholar]
  5. Christian PD, Hanzlik TN, Dall DJ, Gordon KHJ. 1993; Insect viruses: new strategies for pest control.. Edited by Oakeshott J. G., Whitten M. J. Molecular Approaches to Fundamental and Applied Entomology New York: Springer-Verlag.; pp In 1–8
    [Google Scholar]
  6. Felix AM, Terkelsen G. 1973; Total fluorometric amino acid analysis using fluorescamine.. Archives of Biochemistry and Biophysics 157:177–182
    [Google Scholar]
  7. Francki RIB, Francki RIB. 1991; Listing of virus families and groups.. Edited by Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. New York:: Classification and Nomenclature of Viruses: Fifth Report of the International Committee on Taxonomy of Viruses Springer-Verlag.; pp In 80–85
    [Google Scholar]
  8. Gibbs A, Harrison B. 1976; The Principles.. Plant Virology London: Edward Arnold.;
    [Google Scholar]
  9. Hendry DA. 1991; Nodaviridae of invertebrates.. Edited by Kurstak E. Viruses of Invertebrates New York: Marcel Dekker.; pp In 277–285
    [Google Scholar]
  10. Kaesberg P. 1991; Organization of bipartite insect virus genomes: the genome of black beetle virus.. Edited by Rowlands D. J., Mayo M. A., Mahy B. W. J. The Molecular Biology of the Positive Strand RNA Viruses London: Academic Press.; pp In 207–218
    [Google Scholar]
  11. Laemmli UK. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  12. Longworth JF. 1983; Current problems in insect virus taxonomy.. Edited by Matthews R. E. F. A Critical Appraisal of Viral Taxonomy Boca Raton: CRC Press.; pp In 123–138
    [Google Scholar]
  13. Mansi W. 1958; A slide gel diffusion precipitin test.. Nature, London181–1289
    [Google Scholar]
  14. Matsudaira PT. 1989; Purification of proteins and peptides by SDS-PAGE.. Edited by Matsudaira P. T. A Practical Guide to Protein and Peptide Purification for Microsequencing San Diego: Academic Press.; pp In 52–72
    [Google Scholar]
  15. Mayor HD, Hill NO. 1961; Acridine orange staining of a single-stranded DNA bacteriophage.. Virology 14:264–266
    [Google Scholar]
  16. Moore NF. 1991a; The Nudaurelia fS family of insect viruses.. Edited by Kurstak E. Viruses of Invertebrates New York: Marcel Dekker.; pp In 277–285
    [Google Scholar]
  17. Moore NF. 1991b; Identification, pathology, structure, and replication of insect picornaviruses.. Edited by Kurstak E. Viruses of Invertebrates New York: Marcel Dekker.; pp In 287–299
    [Google Scholar]
  18. Newman JFE, Brown F. 1978; Further physicochemical characterization of Nodamura virus. Evidence that the divided genome occurs in a single component.. Journal of General Virology 38:83–95
    [Google Scholar]
  19. Reinganum C. 1991; Tetraviridae.. Edited by Adams J. R., Bonami J. R. Atlas of Invertebrate Viruses Boca Raton: CRC Press.; pp In 553–592
    [Google Scholar]
  20. Rubinstein R. 1979; A nonoccluded virus of the American bollworm, Heliothis armigera (Hubn.).. Phytophylactica 11:179–180
    [Google Scholar]
  21. Sambrook J, Fritsch EF, Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  22. Scott PD. 1985; The estimation of virus density in isopycnic cesium chloride gradients.. Journal of Virological Methods 12:149–160
    [Google Scholar]
  23. Scott PD, Longworth JF, Plus N, Crozier G, Reinganum C. 1981; The biology and ecology of strains of an insect small RNA virus complex.. Advances in Virus Research 26:117–143
    [Google Scholar]
  24. Teakle RE, Jensen JM. 1985; Heliothis punctigera.. Edited by Singh R., Moore R. F. Handbook of Insect Rearing Amsterdam: Elsevier.; pp In 312–322
    [Google Scholar]
  25. Towbin H, Staehelin T, Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
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