1887

Abstract

Rhesus macaques were immunized with purified virus-derived simian immunodeficiency virus of macaques (SIV) 251/32H glycoprotein 130 (gp130) or primed with recombinant vaccinia virus (VV) expressing the gene of the SIV BK28 clone and boosted subsequently with virus-derived gp130. High antibody titres of at least 10 against recombinant gp140 were induced with both vaccines. Analysis of the antibody specificity with a peptide ELISA revealed that different linear epitopes were recognized after administration of virus-derived gp130 compared with those after priming with VV. Antibodies to some epitopes (peptides 10 and 49), which were also found in SIV-infected animals, were induced with both vaccines, whereas antibodies to other regions were induced by only one vaccine preparation. The analysis of the helper T cell response revealed a poor immunogenicity of the virus-derived gp130, whereas priming with VV induced a considerable helper T cell activity in all three vaccinees after the second VV infection. Using synthetic peptides, several epitopes were identified. Our observations show that immunization with a virus-derived gp130 or live recombinant VV induces a considerably different antibody and helper T cell response. These differences in immunogenicity might have important implications for further vaccine development.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-9-1757
1993-09-01
2024-10-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/9/JV0740091757.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-9-1757&mimeType=html&fmt=ahah

References

  1. Benichou S, Legrand R, Nakagawa N, Faure T, Traincard F, Vogt G, Dormont D, Tiollais P, Kieny MP, Madaule P. 1992; Identification of a neutralizing domain in the external envelope glycoprotein of simian immunodeficiency virus.. AIDS Research and Human Retroviruses 8:1165–1170
    [Google Scholar]
  2. Carlson JR, McGraw TP, Reddie E, Yee JL, Rosenthal A, Langlois AJ, Dickover R, Donovan R, Luciw PA, Jennings MB, Gardner MB. 1990; Vaccine protection of rhesus macaques against simian immunodeficiency virus infection.. AIDS Research and Human Retroviruses 6:1239–1246
    [Google Scholar]
  3. Cranage MP, Ashworth LA, Greenaway PJ, MurpheyCorb M, Desrosiers RC. 1992; AIDS vaccine developments.. Nature,London 355:685–686
    [Google Scholar]
  4. Desrosiers RC, Ringler DJ. 1989; Use of simian immunodeficiency viruses for AIDS research.. Intervirology 30:301–312
    [Google Scholar]
  5. Desrosiers RC, Wyand MS, Kodama T, Ringler DJ, Arthur LO, Sehgal PK, Letvin NL, King NW, Daniel MD. 1989; Vaccine protection against simian immunodeficiency virus infection.. Proceedings of the National Academy of Sciences, U.S.A. 86:6353–6357
    [Google Scholar]
  6. Giavedoni LD, Planelles V, Haigwood NL, Ahmad S, Kluge JD, Marthas ML, Gardner MB, Lucrw PA, Yilma TD. 1993; Immune response of rhesus macaques to recombinant simian immunodeficiency virus gpl30 does not protect from challenge infection.. Journal of Virology 67:577–586
    [Google Scholar]
  7. Hartung S, Norley SG, Ennen J, Cichutek K, Arthur LO, Plesker R, Kurth R. 1992; Vaccine protection against SIVmac infection by high- but not low-dose inactivated virus immunogen.. Journal of Acquired Immune Deficiency Syndromes 5:461–468
    [Google Scholar]
  8. Hu SL, Abrams K, Barber GN, Moran P, Zarling JM, Langlois AJ, Kuller L, Morton WR, Benveniste RE. 1992; Protection of macaques against SIV infection by subunit vaccines of SIV envelope glycoprotein gpl60.. Science 255:456–459
    [Google Scholar]
  9. Jones WC, Barnard AL, Slade A, Mills KHG. 1992; Heterogeneity in the recognition of the simian immunodeficiency virus envelope glycoprotein by CD4+ T cell clones from immunized macaques.. Journal of Immunology 149:3120–3126
    [Google Scholar]
  10. Kent KA, Rud E, Corcoran T, Powell C, Thiriart C, Collignon C, Stott EJ. 1992; Identification of two neutralizing and 8 non-neutralizing epitopes on simian immunodeficiency virus envelope using monoclonal antibodies.. AIDS Research and Human Retroviruses 8:1147–1151
    [Google Scholar]
  11. Letvin NL. 1990; Animal models for AIDS.. Immunology Today 11:322–326
    [Google Scholar]
  12. Luke W, Polzien F, Scharf JG, Hunsmann G. 1993b; Biochemical and immunological characterization of micellar complexes of the envelope glycoprotein of a simian immunodeficiency virus isolated from an African green monkey.. Journal of Virological Methods 42:169–180
    [Google Scholar]
  13. Luke W, Schneider J, Schreiner D, Hayami M, Kelliher J, Hunsmann G. 1989; Vaccination of rhesus monkeys with micelles of the external SIVagmTYO-7 glycoprotein gpl30.Edited by R. A. Lerner, H. S. Ginsberg, R. M. Chanock & Brown. New York: Cold Spring Harbor Laboratory.. In Vaccines 89 pp. 219–223 Edited by Lerner R. A., Ginsberg H. S., Chanock Brown R. M. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Miller MA, Murphey-Corb M, Montelaro RC. 1992; Identification of broadly reactive continuous antigenic determinants of simian immunodeficiency virus glycoproteins.. AIDS Research and Human Retroviruses 8:1153–1164
    [Google Scholar]
  15. Mills KHG, Page M, Chan WL, Kitchin P, Stott EJ, Taffs F, Jones W, Rose J, Ling C, Silvera P, Corcoran T, Flanagan B, Burny A, Bex F, Delchambre M, Van Opstal O, Fabry L, Thiriart C, Delers A, DeWilde M, Bruck C. 1992; Protection against SIV infection in macaques by immunization with inactivated virus from the BK28 molecular clone, but not with BK28-derived recombinant env and gag proteins.. Journal of Medical Primatology 21:50–58
    [Google Scholar]
  16. MurpheyCorb M, Martin LN, Davison-Fairburn B, Montelaro R, Miller M, West M, Ohkawa S, Baskin GB, Zhang JY, Putney S, Allison AC, Eppstein D. 1989; A formalin-inactivated whole SIV vaccine confers protection in macaques.. Science 246:1293–1297
    [Google Scholar]
  17. Putkonen P, Thorstensson R, Cranage M, Nilsson C, Ghavamzadeh L, Albert J, Greenaway PJ, Biberfeld G. 1992; A formalin inactivated whole SIVmac vaccine in RIBI adjuvant protects against homologous and heterologous SIV challenge.. Journal of Medical Primatology 21:108–112
    [Google Scholar]
  18. Schneider J, Hunsmann G. 1988; Simian lentiviruses - the SIV group.. AIDS1–9
    [Google Scholar]
  19. Shafferman A, Jahrling PB, Benveniste RE, Lewis MG, Phipps TJ, Eden-McCutchin F, Sadoff J, Eddy GA, Burke DS. 1991; Protection of macaques with a simian immunodeficiency virus envelope peptide vaccine based on conserved human immunodeficiency virus type 1 sequences.. Proceedings of the National Academy of Sciences, U.S.A. 88:7126–7130
    [Google Scholar]
  20. Siegel F, Norley S, Hartung S, Kurth R. 1992; B-cell epitope mapping of the entire SIVagm envelope glycoprotein including fine mapping of immunogenic regions.. Journal of Acquired Immune Deficiency Syndromes 5:583–590
    [Google Scholar]
  21. StahlHennig C, Voss S, Nick S, Petry H, Fuchs D, Wachter H, Coulibaly C, Luke W, Hunsmann G. 1992; Immunization with Tween-ether-treated SIV adsorbed onto aluminium hydroxide protects monkeys against experimental SIV infection.. Virology 186:588–596
    [Google Scholar]
  22. Stott EJ. 1991; Anti-cell antibody in macaques.. Nature, London 353–393
    [Google Scholar]
  23. Stott EJ, Chan WL, Mills KH, Page M, Taffs F, Cranage M, Greenaway P, Luke W, Kitchin P. 1990; Preliminary report: protection of cynomolgus macaques against simian immunodeficiency virus by fixed infected-cell vaccine.. Lancet 336:1538–1541
    [Google Scholar]
  24. Trees R, Schreier MH. 1980; Origin and significance of autoreactive T cells.. Nature, London283–780
    [Google Scholar]
  25. Voss G, Nick S, Stahl-Hennig C, Coulibaly C, Petry H, Luke W, Hunsmann G. 1992; Potential significance of the cellular immune response against the macaque strain of simian immunodeficiency virus (SIVMAC) in immunized and infected rhesus macaques. Journal of General Virology 73:2273–2281
    [Google Scholar]
  26. Voss G, Dittmer U, Nick S, Stahl-Hennig C, Coulibaly C, Petry H, Luke W, Hunsmann G. 1993; Induction of T-cell proliferation in rhesus macaques immunized with Tween-ether-treated SIVmac and loss of proliferative activity after virus infection.. In Animal Models of HIV InfectionEdited by Racz P. Basel: S. Karger (in press);
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-74-9-1757
Loading
/content/journal/jgv/10.1099/0022-1317-74-9-1757
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error