Phage Acm1, generally virulent for the acidophilic facultatively methanol-utilizing strain of MB 58/4, is also capable of lysogenizing its host strain at a low rate. Using amino acid-auxotrophic mutants of MB 58/4 as recipient strains, transduction of His, Leu and Tyr markers could be demonstrated in this system. The ability to prepare transducing lysates by propagation of phage Acm1 on the prototrophic donor strain MB 58/4, the transduction of three different markers as well as the efficiency of transduction, and the occurrence of permutations in the phage genome indicate that phage Acm1 mediates generalized transduction. Phage Acm1 might be a useful tool in genetic studies of methylotrophic .


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