1887

Abstract

On the surface of cowpea protoplasts inoculated with cowpea mosaic virus (CPMV), tubular structures containing virus particles have been found. Such tubular structures are thought to be involved in cell-to-cell movement of CPMV in cowpea plants. To study the involvement of the 58K/48K and capsid proteins of CPMV in the formation of the tubular structures, mutations were introduced into M cDNA clones from which infectious transcripts could be derived. No tubules were found on protoplasts inoculated with a mutant that fails to produce the 48K protein nor with a mutant that has a deletion in the 48K coding region, suggesting that the 48K protein is essential for this process. However, a possible role of the 58K protein in tubule formation could not be excluded. A mutant that fails to produce the capsid proteins did produce tubules and therefore the capsid proteins are not involved in the formation of the tubular structures. Electron microscopic analysis revealed that the tubules produced by this mutant are, apart from the absence of virus particles, morphologically identical to the tubules formed by the wild-type virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-8-1721
1993-08-01
2024-05-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/8/JV0740081721.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-8-1721&mimeType=html&fmt=ahah

References

  1. Eggen R, Van Kammen A. 1988; RNA replication in comoviruses.. RNA Genetics Edited by E. Domigo, J. J. Holland & P. Ahlquist.. Boca Raton: CRC Press.; pp In vol 149–69
    [Google Scholar]
  2. Eggen R., Van Kammen A. 1989; Improvements of the infectivity of in vitro transcripts from cloned cowpea mosaic virus cDNA: impact of terminal nucleotide sequences.. Virology 173:447–455
    [Google Scholar]
  3. Franssen H, Goldbach R, Broekhuijsen M, Moerman M, van Kammen A. 1982; Expression of Middle-Component RNA of Cowpea Mosaic Virus: In Vitro Generation of a Precursor to Both Capsid Proteins by a Bottom-Component RNA-Encoded Protease from Infected Cells. Journal of Virology 41:8–17
    [Google Scholar]
  4. Goldbach R, Rezelman G, van Kammen A. 1982; Expression of Middle-Component RNA of Cowpea Mosaic Virus: In Vitro Generation of a Precursor to Both Capsid Proteins by a Bottom-Component RNA-Encoded Protease from Infected Cells. Journal of Virology 41:8–17
    [Google Scholar]
  5. Holness CL, Lomonossoff GP, Evans D, Maule AJ. 1989; Identification of the initiation codons for translation of cowpea mosaic virus middle component RNA using site-directed mutagenesis of an infectious cDNA clone. Virology 41:8–17
    [Google Scholar]
  6. Kunkel TA. 1985; Rapid and efficient site-specific mutagenesis without phenotypic selection.. Proceedings of the National Academy of Sciences, USA. 82:488
    [Google Scholar]
  7. Rezelman G, Franssen HJ, Goldbach RW, Ie TS, Van Kammen A. 1982; Limits to the Independence of Bottom Component RNA of Cowpea Mosaic Virus. Journal of General Virology 60:335–342
    [Google Scholar]
  8. Sambrook J, Fritsch EF, Maniatis T. 1989 Molecular Cloning: A Laboratory Manual,, 2nd edn.. New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  9. van Lent J, Wellink J, Goldbach R. 1990; Evidence for the Involvement of the 58K and 48K Proteins in the Intercellular Movement of Cowpea Mosaic Virus. Journal of General Virology 71:219–223
    [Google Scholar]
  10. van Lent J, Storms M, van der Meer F, Wellink J, Goldbach R. 1991; Tubular structures involved in movement of cowpea mosaic virus are also formed in infected cowpea protoplasts. Journal of General Virology 72:2615–2623
    [Google Scholar]
  11. Vos P, Verver J, Jaegle M, Wellink J, van Kammen A, Goldbach R. 1991; Two viraj proteins involved in the proteolytic processing of the cowpea mosaic virus polyproteins. Nucleic Acids Research 16:1967–1985
    [Google Scholar]
  12. Wellink J, Jaegle M, Prinz H, Van Kammen A, Goldbach R. 1989; Expression of the Middle Component RNA of Cowpea Mosaic Virus in vivo. Journal of General Virology 68:2577–2585
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-8-1721
Loading
/content/journal/jgv/10.1099/0022-1317-74-8-1721
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error