@article{mbs:/content/journal/jgv/10.1099/0022-1317-74-8-1715, author = "Finnen, Renée L. and Rochon, D′Ann M.", title = "Sequence and structure of defective interfering RNAs associated with cucumber necrosis virus infections", journal= "Journal of General Virology", year = "1993", volume = "74", number = "8", pages = "1715-1720", doi = "https://doi.org/10.1099/0022-1317-74-8-1715", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-74-8-1715", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The presence of symptom-attenuating defective interfering (DI) RNAs in a laboratory culture of cucumber necrosis tombusvirus (CNV) was confirmed. Sequencing of cDNA clones of these DI RNAs revealed that CNV DI RNAs retained sequences from the CNV 5′-untranslated and 3′-terminal regions as well as a portion of the coding region for the 92K protein. Similar sequence arrangements were also observed in symptom-attenuating DI RNAs generated de novo from synthetic wild-type CNV transcripts. A comparison of the sequences and biological activities of these CNV DI RNAs is presented. In co-infections of synthetic wild-type CNV and CNV DI RNAs, prominent RNA species of a higher M r than the DI RNA used in the co-infection were found. The possible nature of these RNA species is discussed.", }