1887

Abstract

A gene was identified in camelpox virus strain CP-1 that is similar to the 160K gene of cowpox virus strain Brighton (BR) that encodes the A-type inclusion body protein (ATIP). The CP-1 gene was mapped, sequenced, and the presence of the ATIP-specific mRNA was demonstrated. The open reading frame [2178 nucleotides (nt)] was found at a similar position in the CP genome as the one reported for the cowpox virus 160K ATI gene. DNA sequence comparison revealed a deletion of two adjacent adenine residues relative to cowpox virus BR, generating a reading frame shift accompanied by the formation of a translational stop codon. An identical deletion has been described for vaccinia virus strain Western Reserve. The DNA sequence of the corresponding region of monkeypox virus strain Copenhagen revealed a deletion leading to a putative stop codon 75 nt upstream of the same stop codons in the camelpox and vaccinia virus genes. These findings are consistent with the expression of truncated ATIPs, of 94K in vaccinia and camelpox viruses and of 92K in monkeypox virus. In addition, a deletion of 789 bp could be localized downstream of the ATI open reading frame in camelpox virus isolates of different origin. This causes the transcription of a shortened ATI-specific mRNA (3·7 kb) relative to vaccinia and cowpox viruses (both 4·5 kb). The similarity observed in ATIP-encoding and flanking sequences might suggest that vaccinia and camelpox viruses are descended from a common ancestor.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-8-1679
1993-08-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/8/JV0740081679.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-8-1679&mimeType=html&fmt=ahah

References

  1. Amegadzie BY, Sisler JR, Moss B. 1992; Frame-shift mutations within the vaccinia virus A-type inclusion protein gene.. Virology 186:777–782
    [Google Scholar]
  2. Chomczynski P, Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.. Analytical Biochemistry 162:156–159
    [Google Scholar]
  3. DeCarlos A, Paez E. 1991; Isolation and characterization of mutants of vaccinia virus with a modified 94 kDa inclusion protein.. Virology 185:768–778
    [Google Scholar]
  4. Devereux J, Haeberli P, Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12:387–395
    [Google Scholar]
  5. Downie AW. 1939; Study of the lesions produced experimentally by cowpox virus.. Journal of Pathology and Bacteriology 48:361
    [Google Scholar]
  6. Fenner F, Witter R, Dumbell KR. 1989; The Orthopox-viruses.. New York & London: Academie Press.;
    [Google Scholar]
  7. Funahashi S, Sato T, Shida H. 1988; Cloning and characterization of the gene encoding the major protein of the A-type inclusion body of cowpox virus.. Journal of General Virology 69:35–47
    [Google Scholar]
  8. Goebel SJ, Johnson GP, Perkus ME, Davis SW, Winslow JP, Paoletti E. 1990; The complete DNA sequence of vaccinia virus.. Virology 179:247–266
    [Google Scholar]
  9. Hattori M, Sakaki Y. 1986; Dideoxy sequencing method using denatured plasmid templates.. Analytical Biochemistry 152:232–238
    [Google Scholar]
  10. Ichihashi Y, Matsumoto S, Dales S. 1971; Biogenesis of poxviruses: rôle of A-type inclusions and host cell membranes in virus dissemination.. Virology 46:507–532
    [Google Scholar]
  11. Kato S, Takahashi M, Kameyama S, Kamahora J. 1959; A study on the morphological and cyto-immunological relationship between the inclusions of variola, cowpox, rabbitpox, vaccinia (variola origin) and vaccinia IHD and a consideration of the terni ‘Guamieri body’.. Biken’s Journal 2:353–363
    [Google Scholar]
  12. Kitamoto N, Goto E, Tanimoto S, Tanaka T, Miyamoto H, Wakamija N, Ikuta K, Ueda S, Kato S. 1984; Cross-reactivity between cowpox and vaccinia viruses with monoclonal antibodies.. Archives of Virology 82:129–136
    [Google Scholar]
  13. Kitamoto N, Tanimoto S, Hiroi K, Miyamoto H, Wakamiya N, Ueda S, Kato S. 1986a; Cross-reactivity among cowpox, ectromelia and vaccinia viruses with monoclonal antibodies recog-nizing distinct antigenic determinants in A-type inclusion bodies.. Archives of Virology 91:357–366
    [Google Scholar]
  14. Kitamoto N, Tanimoto S, Hiroi K, Tanaka T, Miyamoto H, Wakamiya N, Ikuta K, Ueda S, Kato S. 1986b; Polypeptide analysis with monoclonal antibodies of A-type inclusion bodies induced by cowpox virus.. Archives of Virology 152:232–238
    [Google Scholar]
  15. Knight JC, Goldsmith CS, Tamin A, Regnery RL, Regnery DC, Esposito JJ. 1992; Further analyses of the orthopoxviruses volepox virus and raccoon poxvirus.. Virology 190:423–133
    [Google Scholar]
  16. Mahnel H. 1974; Labordilïerenzierung der Orthopockenviren.. Zentralblatt fur Veterinàrmedizin, Reihe B 21:242–258
    [Google Scholar]
  17. Marchal J. 1930; Infectious ectromelia. A hitherto undescribed virus disease of mice.. Journal of Pathology and Bacteriology33–713
    [Google Scholar]
  18. Moss B. 1990; Poxviridae and their replication.. Virology Edited by B. N. Fields & D. M. Knipe.. , 2nd edn.. New York: Raven Press.; pp In 2079–2111
    [Google Scholar]
  19. Patel DD, Pickup DJ. 1987; Messenger RNAs of a strongly-expressed late gene of cowpox virus contain 5′-terminal poly(A) sequences.. EMBO Journal 6:3787–3794
    [Google Scholar]
  20. Patel DD, Pickup DJ, Joklik WK. 1986; Isolation of cowpox virus A-type inclusions and characterization of their major protein component.. Virology 149:174–189
    [Google Scholar]
  21. Patel DD, Ray CA, Drucker RP, Pickup DJ. 1988; A poxvirus-derived vector that directs high levels of expression of cloned genes in mammalian cells.. Proceedings of the National Academy of Sciences, U.S.A. 85:9431–9435
    [Google Scholar]
  22. Ramyar H, Hessami M. 1972; Isolation, cultivation and characterization of camelpox virus.. Zentralblatt fur Veterinar-medizin, Reihe B 19:182–189
    [Google Scholar]
  23. Sambrook J, Fritsch EF, Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. New York: Cold Spring Harbor Laboratory.; 19182–189
    [Google Scholar]
  24. Sanger F, Nicklen S, Coulson AR. 1977; DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  25. Shida H, Tanabe K, Matsumoto S. 1977; Mechanism of virus occlusion into A-type inclusion during poxvirus infection.. Virology 76:217–233
    [Google Scholar]
  26. von Magnus P, Andersen EK, Petersen BK, Petersen BK, Birch-Andersen A. 1959; A pox-like disease in cynomolgus monkeys.. Acta pathologica et microbiologica scandinavica 46:146
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-8-1679
Loading
/content/journal/jgv/10.1099/0022-1317-74-8-1679
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error