1887

Abstract

The human monocytic cell line THP-1 was used as a model to study the mechanism of infection in the monocyte/macrophage, a natural target of lymphocytic choriomeningitis virus (LCMV) infection . Both the virulent strain, LCMV. WE, and the avirulent strain, LCMV. ARM, infected THP-1 cells, but did not stimulate THP-1 cells to secrete interleukin 1 (IL-1) or tumour necrosis factor (TNF-). When lipopolysaccharide (LPS) was added to THP-1 cells together with LCMV, an 80 to 90% reduction in the number of infected cells (measured by immunofluorescence) and a 90% reduction in viral plaques was observed 5 to 6 days postinfection. Neither interferon (IFN-) nor IFN- were detected in supernatants from THP-1 cells after the addition of LCMV, LPS, or LPS plus LCMV. In contrast, the same levels of IL-1 and TNF-α were observed in the presence of LPS and LCMV, or LPS alone. However, antibodies to IL-1, TNF-, interleukin 6 and IFN- did not block the antiviral effect of LPS. In kinetic studies, LPS added 1 day after adding LCMV to THP-1 cells was still effective in reducing the number of infected cells. Our findings suggest that LPS alters cellular metabolism, possibly through the induction of IFN-, and that IFN-α in the absence of LPS suppresses virus production.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-8-1653
1993-08-01
2022-10-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/8/JV0740081653.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-8-1653&mimeType=html&fmt=ahah

References

  1. Ambrosio M, Vallejos A, Saavedra C, Maiztegui JI. 1990; Junin virus replication in peripheral blood mononuclear cells of patients with Argentine haemorrhagic fever.. Acta virologica 34:58–63
    [Google Scholar]
  2. Beutler B, Cerami A. 1986; Cachectin and tumour necrosis factor as two sides of the same biological coin.. Nature, London 320:584–588
    [Google Scholar]
  3. Carney WP, Hirsch MS. 1981; Mechanism of immunosuppression in cytomegalovirus mononucleosis. II. Virus-monocyte interactions.. Journal of Infectious Diseases 144:47–54
    [Google Scholar]
  4. Domurat F, Roberts NJ, Jr, Walsh EE, Dagan R. 1985; Respiratory syncytial virus infection of human mononuclear leukocytes in vitro and in vivo.. Journal of Infectious Diseases 152:895–902
    [Google Scholar]
  5. Frei K, Malipiero UV, Leist TP, Zinkernagel RM, Schwab ME, Fontana A. 1989; The cellular source and function of interleukin 6 produced in the central nervous system in viral diseases.. European Journal of Immunology 19:689–694
    [Google Scholar]
  6. Genovesi EV, Peters CJ. 1987; Susceptibility of inbred Syrian golden hamsters (Mesocricetus auratus) to lethal disease by lymphocytic choriomeningitis virus.. Proceedings of the Society for Experimental Biology and Medicine 185:250–261
    [Google Scholar]
  7. Halstead SB, O’Rourke EJ, Allison AC. 1977; Dengue viruses and mononuclear phagocyte. II. Identity of blood and tissue leukocytes supporting in vitro infection.. Journal of Experimental Medicine 146:218–229
    [Google Scholar]
  8. Hayes MP, Enterline JC, Gerrard TL, Zoon KC. 1991; Regulation of interferon production by human monocytes: requirements for priming for LPS-induced production.. Journal of Leukocyte Biology 50:176–181
    [Google Scholar]
  9. Jacobs RP, Cole GA. 1976; LCMV induced immunosuppression: a virus-induced macrophage defect.. Journal of Immunology 117:1004–1009
    [Google Scholar]
  10. Kornbluth RS, Oh PS, Munis JR, Cleveland PH, Richman DD. 1989; Interferons and bacterial lipopolysaccharide protect macrophages from productive infection by human immunodeficiency virus in vitro.. Journal of Experimental Medicine 169:1137–1151
    [Google Scholar]
  11. Krakauer T, Oppenheim JJ. 1992; Interleukin.. Human Protein Data. I. Installment. Edited by A. Haeberli. Weinheim: VCH Verlageges..
    [Google Scholar]
  12. Kurane I, Ennis FA. 1987; Induction of interferon α from human lymphocytes by autologous, dengue virus-infected monocytes.. Journal of Experimental Medicine 166:999–1010
    [Google Scholar]
  13. Lengyel P. 1982; Biochemistry of IFNs and their action.. Annual Review of Biochemistry 51:251–282
    [Google Scholar]
  14. Muñoz A, Carrasco L. 1984; Formation of non-infective herpesvirus particles in cultured cells treated with human interferon.. Journal of General Virology 65:1069–1078
    [Google Scholar]
  15. Murphy FA, Walker DA. 1978; Arenaviruses: persistent infection and viral survival in reservoir hosts.. In Viruses and Environmen Chapter 10 Edited by E. Kurstak & K. Maramorosch.. New York: Academic Press.;
    [Google Scholar]
  16. Nicholson JK, Cross GD, Callaway CS, McDougal JS. 1986; In vitro infection of human monocytes with the human T lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV).. Journal of Immunology 137:323–329
    [Google Scholar]
  17. Oldstone MBA. 1989; Viral persistence.. Cell 56:517–520
    [Google Scholar]
  18. Peters CJ, Jahrling PB, Liu CT, Kenyon RH, McKee KT, Barrera-Oro JG. 1987; Experimental studies of arenaviral hemorrhagic fevers.. Current Topics in Microbiology and Immunology 134:5–68
    [Google Scholar]
  19. Rosenthal AS. 1980; Regulation of the immune response – role of the macrophage.. New England Journal of Medicine 303:1153–1159
    [Google Scholar]
  20. Rubinstein S, Familletti PC, Pestka S. 1981; Convenient assay for interferons.. Journal of Virology 37:755–758
    [Google Scholar]
  21. Samuel CE. 1991; Antiviral actions of interferon. Interferon-regulated cellular proteins and their surprisingly selective antiviral activities.. Virology 183:1–11
    [Google Scholar]
  22. Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K. 1980; Establishment and characterization of human acute monocytic leukemia cell line (THP-1).. International Journal of Cancer 26:171–176
    [Google Scholar]
  23. Van Der Logt JTM, Van Loon AM, Van Der Veen J. 1980; Replication of rubella virus in human mononuclear blood cells.. Infection and Immunity 27:309–314
    [Google Scholar]
  24. Van Snick J. 1990; Interleukin 6: an overview.. Annual Review of Immunology 8:253–278
    [Google Scholar]
  25. Wong HWG, Goeddel DV. 1986; Tumour necrosis factor a and β inhibit virus replication and synergize with interferons.. Nature, London 323:819–822
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-8-1653
Loading
/content/journal/jgv/10.1099/0022-1317-74-8-1653
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error