1887

Abstract

A new gene of the baculovirus multicapsid nuclear polyhedrosis virus (OpMNPV) has been identified that encodes a highly basic 8.9K protein. The gene called p8.9 is expressed as a 0.5 kb transcript by 1 h post-infection and initiates at an early gene motif. The promoter of the 0.5 kb transcript has two upstream elements, repeats I and II, which are similar to motifs previously characterized in the OpMNPV IE-2 gene and the nuclear polyhedrosis virus IE-N and PE38 genes. A second p8.9 transcript expressed from 8 to 72 h post-infection was shown to initiate 634 bp upstream from the early gene motif in a region that has no similarity to any previously described baculovirus promoter or initiation site. Transient assays utilizing a reporter gene have shown that the p8.9 early promoter is active in a (LD652Y) and (Sf9) cell lines in the absence of other viral factors. In addition, it was also demonstrated that the p8.9 promoter is trans-activated by the OpMNPV IE-2 and p34 genes, but not by IE-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-8-1591
1993-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/8/JV0740081591.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-8-1591&mimeType=html&fmt=ahah

References

  1. Blissard GW, Rohrmann GF. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus.. Virology 170:537–555
    [Google Scholar]
  2. Blissard GW, Rohrmann GF. 1990; Baculovirus diversity and molecular biology.. Annual Review of Entomology 35:127–155
    [Google Scholar]
  3. Blissard GW, Rohrmann GF. 1991; Baculovirus gp64 gene expression : analysis of sequences modulating early gene transcription and transactivation by IE1.. Journal of Virology 65:5820–5827
    [Google Scholar]
  4. Calnan BJ, Biancalana S, Hudson D, Frankel AD. 1991; Analysis of arginine-rich peptides from the HIV Tat protein reveals unusual features of RNA-protein récognition.. Genes & Development 5:201–201
    [Google Scholar]
  5. Carson DD, Summers MD, Guarino LA. 1991; Molecular analysis of a baculovirus regulatory gene.. Virology 182:279–286
    [Google Scholar]
  6. Chisholm GE, Henner DJ. 1988; Multiple early transcripts and splicing of the Autographa californien nuclear polyhedrosis virus IE-1 gene.. Journal of Virology 62:3193–3200
    [Google Scholar]
  7. Devereux J, Haeberli P, Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12:587–395
    [Google Scholar]
  8. Friesen PD, Miller LK. 1986; The régulation of baculovirus gene expression.. Current Topics in Microbiology and Immunology 131:31–49
    [Google Scholar]
  9. Fuchs LY, Woods MS, Weaver RF. 1983; Viral transcription during Autographa californica nuclear polyhedrosis virus infection: a novel RNA polymerase induced in infected Spodoptera frugiperda cells.. Journal of Virology 170:641–646
    [Google Scholar]
  10. Grula MA, Buller PL, Weaver RF. 1981; Alphaamanitin-resistant viral RNA synthesis in nuclei isolated from nuclear polyhedrosis virus-infected Heliothis zea larvae and Spodotera frugiperda cells.. Journal of Virology 38:916–921
    [Google Scholar]
  11. Guarino LA, Smith M. 1989; Régulation of delayed-early gene transcription by dual TATA boxes.. Journal of Virology 66:3733–3739
    [Google Scholar]
  12. Guarino LA, Summers MD. 1987; Nucléotide sequence and temporal expression of a baculovirus regulatory gene.. Journal of Virology 61:2091–2099
    [Google Scholar]
  13. Hoopes RH, Rohrmann GF. 1991; In vitro transcription of baculovirus immédiate early genes: accurate mRNA initiation by nuclear extracts from both insect and human cells.. Proceedings of the National Academy of Sciences, U.S.A. 88:4513–4517
    [Google Scholar]
  14. Huh NE, Weaver RF. 1990; Identifying the RNA polymerases that synthesize spécifie transcripts of the Autographa californica nuclear polyhedrosis virus.. Journal of General Virology 71:195–201
    [Google Scholar]
  15. Krappa R, Knebel-Morsdorf D. 1991; Identification of the very early transcribed baculovirus gene PE-38.. Journal of Virology 65:805–812
    [Google Scholar]
  16. Krappa R, Behn-Krappa A, Doerfler W, Knebel-Morsdorf D. 1992; Differential factor binding at the promoter of early baculovirus gene PE38 during viral infection: GATA motif is recognized by an insect protein.. Journal of Virology 66:3494–3503
    [Google Scholar]
  17. Leisy DJ, Rohrmann GF, Beaudreau GS. 1984; Conservation of genome organization in two multicapsid nuclear polyhedrosis viruses.. Journal of Virology 52:699–702
    [Google Scholar]
  18. Marck C. 1988; DNA Strider’: a ‘C’ program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.. Nucleic Acids Research 15:1829–1836
    [Google Scholar]
  19. Nissen MS, Friesen PD. 1989; Molecular analysis of the transcriptional regulatory région of an early baculovirus gene.. Journal of Virology 63:493–503
    [Google Scholar]
  20. Pabo CW, Sauer RT. 1992; Transcription factors: structural families and principles of DNA récognition.. Annual Review of Biochemistry 61:1053–1095
    [Google Scholar]
  21. Quant-Russell RL, Pearson MN, Rohrmann GF, Beaudreau GS. 1987; Characterization of baculovirus plO synthesis using monoclonal antibodies.. Virology 160:9–19
    [Google Scholar]
  22. Rohrmann GF. 1992; Baculovirus structural proteins.. Journal of General Virology 73:749–761
    [Google Scholar]
  23. Russell RL. , Rohrmann GF. 1990; The p6-5 gene région of a nuclear polyhydrosis virus of Orgyia pseudotsugata : DNA sequence and transcriptional analysis of four late genes.. Journal of General Virology 71:551–560
    [Google Scholar]
  24. Sambrook J, Fritsch EF, Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Shapira SK, Chou J, Richaud FV, Casabadan MJ. 1983; New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of β-galactosidase.. Gene 25:71–82
    [Google Scholar]
  26. Summers MD, Smith GE. 1987; A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures.. Texas Agricultural Experiment Station, bulletin no. 1555:537–555
    [Google Scholar]
  27. Theilmann DA, Stewart S. 1991; Identification and characterization of the IE-1 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus.. Virology 180:492–508
    [Google Scholar]
  28. Theilmann DA, Stewart S. 1992a; Molecular analysis of the trans-activating IE-2 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus.. Virology 187:84–96
    [Google Scholar]
  29. Theilmann DA, Stewart S. 1992b; Tandemly repeated sequence at the 3′ end of the IE-2 gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element.. Virology 187:97–106
    [Google Scholar]
  30. Thomas PS. 1983; Hybridization of denatured RNA transferred or dotted to nitrocellulose paper.. Methods in Enzymology 100:255–266
    [Google Scholar]
  31. Toneguzzo F, Glynn S, Leci E, Mijolsness S, Hayday A. 1988; Use of a chemically modified T7 polymerase for manual and automated sequencing of supercoiled DNA.. BioTechniques 6:460–469
    [Google Scholar]
  32. Wilson ME. 1988; A synthetic peptide to the predicted 6-9K translation product of the 7/mdII I-H/GoRI-D région of the AcMNPV genome induces antibodies to the basic DNA-binding protein.. Virus Research 9:21–31
    [Google Scholar]
  33. Wilson ME, Mainprize TH, Friesen PD, Miller LK. 1987; Location, transcription, and sequence of a baculovirus gene encoding a small arginine-rich polypeptide.. Journal of Virology 61:661–661
    [Google Scholar]
  34. Xie W, Rothblum LI. 1991; Rapid, small-scale RNA isolation from tissue culture cells.. BioTechniques 11:324–327
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-8-1591
Loading
/content/journal/jgv/10.1099/0022-1317-74-8-1591
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error