1887

Abstract

The core of a unique linear neutralization epitope (G5) on the glycoprotein of rabies virus, recognized by a virus-neutralizing mouse monoclonal antibody (MAb 6-15C4), was determined by Pepscan analysis. The G5 epitope was defined as an octapeptide (LHDFRSDE). The contribution of the individual amino acids of the G5 epitope to the binding of MAb 6-15C4 was analysed with a set of synthetic peptides in which the individual amino acids had been replaced in turn by each of the other 19 naturally occurring amino acids. Five amino acids of the octapeptide proved to be essential for the binding of MAb 6-15C4. The conservation of the G5 epitope within the glycoprotein of the different rabies virus strains sequenced to date proved to be absolute at the amino acid level. Studies concerning the immunodominance of the G5 epitope were carried out by determining the presence of G5 epitope-specific serum antibodies in vaccinated humans and mice, and by determining the frequency of G5 epitope-specific B lymphocytes in the blood of vaccinated humans. These studies indicated that antibodies to the G5 epitope constitute a minor population of the rabies virus-specific serum antibodies induced by rabies vaccination.

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1993-08-01
2024-03-29
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References

  1. Anilionis A, Wunner WH, Curtis PJ. 1981; Structure of the glycoprotein gene in rabies virus.. Nature, London 294:275–278
    [Google Scholar]
  2. Benmansour A, Brahimi M, Tuffereau C, Coulon P, Lafay F, Flamand A. 1992; Rapid sequence evolution of street rabies glycoprotein is related to the highly heterogeneous nature of the viral population.. Virology 187:33–45
    [Google Scholar]
  3. Bunschoten H, Gore M, Claassen C, I. J. T. H. M. P, UytdeHaag FG. C. M, Dietzschold B, Wunner WH, Osterhaus AD. M. E. 1989; Characterization of a new virus-neutralizing epitope that denotes a sequential determinant on the rabies virus glycoprotein.. Journal of General Virology 70:291–298
    [Google Scholar]
  4. Conzelman KK, Cox JH, Schneider LG, Thiel HJ. 1990; Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19.. Virology 175:485–499
    [Google Scholar]
  5. Dietzschold B, Gore M, Ertl H, Otvos L, Jr, Koprowski H. 1989; Analysis of protective immune mechanisms induced by rabies nucleoprotein.. In Genetics and Pathogenicity of Negative-strand Viruses pp. 295–309 Edited by B. W. J. Mahy & D. Kolakofsky. New York: Elsevier;
    [Google Scholar]
  6. Dietzschold B, Gore M, Casali H, Otvos P, Ueki Y, Rupprecht CE, Notkins AL, Osterhaus AD. M. E. 1990a; Biological characterization of human monoclonal antibodies to rabies virus.. Journal of Virology 64:3087–3090
    [Google Scholar]
  7. Dietzschold B, Gore M, Marchadier D, Niu H.-S, Bunschoten HM, Otvos L, Wunner WH, Ertl HC. J, Osterhaus AD. M. E, Koprowski H. 1990b; Structural and immunological characterization of a linear virus neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine.. Journal of Virology 64:3804–3809
    [Google Scholar]
  8. Doolittle RF. 1985; Proteins.. Scientific American 352:74–85
    [Google Scholar]
  9. Flamand A, Wiktor TJ, Koprowski H. 1980; Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. II. The glycoprotein. II.. The glycoprotein. Journal of General Virology 48:105–109
    [Google Scholar]
  10. Geysen HM, Meloen RH, Barteling SJ. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.. Proceedings of the National Academy of Sciences, U.S.A. 81:3998–4002
    [Google Scholar]
  11. Geysen HM, Barteling SJ, Meloen RH. 1985; Small peptides induce antibodies with sequence and structural requirements for binding antigen comparable to antibodies raised against the native protein.. Proceedings of the National Academy of Sciences, U.S.A. 82:178–182
    [Google Scholar]
  12. Groen J, Dalrymple J, Fisher-Hoch S, Jordans JG. M, Clement JP, Osterhaus AD. M. E. 1992; Serum antibodies to structural proteins of hantavirus arise at different times after infection.. Journal of Medical Virology 37:283–287
    [Google Scholar]
  13. Jerne NK. 1974; Towards a network theory of the immune system.. Annales d’lmmunologie 125C:373–389
    [Google Scholar]
  14. Lafon M, Wiktor TJ, MacFarlan RI. 1983; Antigenic sites on the CVS rabies virus glycoprotein. Analysis with monoclonal antibodies.. Journal of General Virology 64:843–851
    [Google Scholar]
  15. Lafon M, Ideler I, Wunner WH. 1984; Investigation of the antigenic structure of rabies virus glycoprotein by monoclonal antibodies.. Developments in Biological Standardization 57:219–225
    [Google Scholar]
  16. Morimoto K, Ohkubo A, Kawai A. 1989; Structure and transcription of the glycoprotein gene of attenuated HEP-Flury strain of rabies virus.. Virology 173:465–477
    [Google Scholar]
  17. Sikes RK, Cleri WF, Koprowski H, Wiktor TJ, Kaplan MM. 1971; Effective protection of monkeys against death from street virus by post-exposure administration of tissue culture rabies vaccine.. Bulletin of the World Health Organization 45:1–11
    [Google Scholar]
  18. Tordo N, Poch O, Ermine F. 1986; Walking along the rabies genome: is the large G-L intergenic region a remnant gene.. Proceedings of the National Academy of Sciences, U.S.A. 83:3914–3918
    [Google Scholar]
  19. Turner GS. 1985; Immune response after rabies vaccination: basic aspects.. Annales de TInstitut Pasteur Virologie 136E:453–460
    [Google Scholar]
  20. UytdeHaag FG. C. M, Loggen HG, Logtenberg T, Licht-veld RA, van Steenis G, van Asten JA. A. M, Osterhaus AD. M. E. 1985; Human peripheral blood lymphocytes from recently vaccinated individuals produce both homotypic and heterotypic neutralizing antibody upon in vitro-stimulation with one type of poliovirus.. Journal of Immunology 135:3094–3101
    [Google Scholar]
  21. van der Heijden RW. J, Bunschoten H, Pascual V, UytdeHaag FG. C. M, Osterhaus AD. M. E, Capra JD. 1990; Nucleotide sequence of a human monoclonal anti-idiotypic antibody specific for a rabies virus neutralizing monoclonal idiotypic antibody reveals extensive somatic variability suggestive of an antigen driven immune response.. Journal of Immunology 144:2835–2839
    [Google Scholar]
  22. van der Heijden RW. J, Bunschoten H, Hoek A, van Es J, Punter M, Osterhaus AD. M. E, UytdeHaag FG. C. M. 1991; A human CD5+ B cell clone that secretes an idiotype specific high affinity IgM monoclonal antibody.. Journal of Immunology 146:1503–1508
    [Google Scholar]
  23. van Wezel AL, van Steenis G, Hannik CA, Cohen H. 1978; New approaches to the production of concentrated and purified inactivated polio and rabies tissue culture vaccines.. Developments in Biological Standardization 41:159–168
    [Google Scholar]
  24. Wiktor TJ, Koprowski H. 1978; Monoclonal antibodies against rabies virus produced by somatic cell hybridization: detection of antigenic variants.. Proceedings of the National Academy of Sciences, U.S.A. 75:3938–3942
    [Google Scholar]
  25. Wiktor TJ, MacFarlan RI, Reaganr KJ, Dietzschold B, Wunner WH, Kieny M.-P, Lathe R, Lecocq J.-P, Mackett M, Moss B, Koprowski H. 1984; Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene.. Proceedings of the National Academy of Sciences, U.S.A. 81:7194–7194
    [Google Scholar]
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