1887

Abstract

Immunofluorescence analysis of HeLa cells incubated with human adenovirus serotype 2 (Ad2) inoculum suggested that Ad2 receptors co-localized with the receptors of fibronectin (FN) and vitronectin (VN) at the cell surface. Ad2 adsorption also resulted in the occurrence of intracytoplasmic actin cables with submembranal anchorage. The cell binding of Ad2 virions, pentons and fibres was found to be efficiently inhibited by concanavalin A, laminin, anti-FN and anti-VN antibodies. Arginine-glycine-aspartyl tripeptide (RGD) and other related peptides reproducing cellular attachment sequences of adhesion proteins also competed with Ad2 for cell adsorption, and drastically reduced the virus progeny yield at the end of the infectious cycle. Data from binding competition assays with Ad2 virions showed that the apparent affinity constants of RGD motif-containing peptides for Ad2 receptor ranged from 0·75 × 10 to 2.2 × 10 , with a number of peptide recognizing sites varying from 1·5 × 10 to 9 × 10 per cell for the different peptides studied. Polypeptide analysis of labelled plasma membrane fractions isolated after cross-linking to unlabelled Ad2 virions showed three major protein species with apparent of 130K, 60K and 44K, respectively, reacting with anti-FN and anti-VN antibodies. These results suggested that Ad2 and extracellular matrix proteins recognize similar adhesion sequences at the surface of HeLa cells, or alternatively that integrins and Ad2 receptors have overlapping ligand specificity.

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1993-08-01
2021-10-18
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References

  1. Albelda SM, Buck C. 1990; Integrin and other cell adhesion molecules.. Federation of American Societies for Experimental Biology Journal 4:2868–2880
    [Google Scholar]
  2. Albritton LM, Tseng L, Scadden D, Cunningham JM. 1989; A putative murine ecotropic retrovirus receptor gene encodes a multiple membrane-spanning protein and confers susceptibility to virus infection.. Cell 57:659–666
    [Google Scholar]
  3. Belin MT, Boulanger P. 1985; Cytoskeletal proteins associated with intracytoplasmic human adenovirus at an early stage of infection.. Experimental Cell Research 160:356–370
    [Google Scholar]
  4. Belin MT, Boulanger P. 1987; Processing of vimentin occurs during the early stages of adenovirus infection.. Journal of Virology 61:2559–2556
    [Google Scholar]
  5. Bergelson JM, Shepley MP, Chan BM. C, Hemler ME, Finberg RW. 1992; Identification of the integrin VLA-2 as a receptor for echovirus 1.. Science 255:1718–1720
    [Google Scholar]
  6. Boudin ML, Boulanger P. 1982; Assembly of adenovirus penton base and fiber.. Virology 116:589–604
    [Google Scholar]
  7. Boudin ML, D’Halluin J, Cousin C, Boulanger P. 1980; Human adenovirus type 2 protein Ilia. II. Maturation and encapsidation.. Virology 101:144–151
    [Google Scholar]
  8. Boulanger P, Lonberg-Holm K. 1981; Components of non-enveloped viruses which recognize receptors.. In Receptors and Recognition, Series B vol 8 Virus Receptors, Part 2, Animal Viruses pp. 23–46 Edited by K. Lonberg-Holm & L. Philipson.. New York: Chapman & Hall;
    [Google Scholar]
  9. Boulanger P, Philipson L. 1981; Membrane components interacting with non-enveloped viruses.. In Receptors and Recognition, Series B vol 8 Virus Receptors, Part 2, Animal Viruses pp. 117–139 Edited by K. Lonberg-Holm & L. Philipson. New York: Chapman & Hall;
    [Google Scholar]
  10. Boulanger P, Houdret N, Scharfman A, Lemay P. 1972; The role of surface sialic acid in adenovirus-cell adsorption.. Journal of General Virology 16:429–434
    [Google Scholar]
  11. Bretscher MS. 1992a; Cells can use their transferrin receptors for locomotion.. EMBO Journal 11:383–289
    [Google Scholar]
  12. Bretscher MS. 1992 b; Circulating integrins: α5β1, α6β4 and Mac-1, but not α3β1, α4β1 or LFA-1.. EMBO Journal 11:405–410
    [Google Scholar]
  13. Burridge K. 1987; Substrate adhesion in normal and transformed fibroblasts: organization and regulation of cytoskeletal membrane and extracellular matrix components at focal contacts.. Cancer Reviews 4:18–78
    [Google Scholar]
  14. Chardonnet Y, Dales S. 1970; Early events in the interaction of adenovirus with HeLa cells.. intracellular release of the DNA genome. Virology 40:462
    [Google Scholar]
  15. Cotten M, Wagner E, Zatloukal K, Phillips S, Curiel DT, Birnsttel ML. 1992; High-efficiency receptor-mediated delivery of small and large (48 kilobase) gene constructs using the endosome-disruption activity of defective or chemically inactivated adenovirus particles.. Proceedings of the National Academy of Sciences, U.S.A. 89:6094–6098
    [Google Scholar]
  16. Crowell RL, Landau BJ, Hsu KHL, Schultz M. 1988; Perspectives on cellular receptors as determinants of viral tropism.. In Coxsackieviruses: A General Update pp. 51–59 Edited by M. Bendinelli & H. Friedman.. New York: Plenum Press;
    [Google Scholar]
  17. Curiel DT, Agarwal S, Wagner E, Cotten M. 1991; Adenovirus enhancement of transferrin-polylysine-mediated gene delivery.. Proceedings of the National Academy of Sciences, U.S.A. 88:8850–8854
    [Google Scholar]
  18. Dalgleish AG, Beverly PCL, Clapham PR, Crawford DH, Greaves MF, Weiss RA. 1984; The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.. Nature, London 312:763–767
    [Google Scholar]
  19. Defer AG, Belin MT, Caillet-Boudin M, Boulanger P. 1990; Human adenovirus-host cell interactions: a comparative study with members of subgroups B and C.. Journal of Virology 64:3661–3673
    [Google Scholar]
  20. D’Halluin JC, Martin GR, Torpier G, Boulanger P. 1978; Adenovirus type 2 assembly analyzed by reversible crosslinking of labile intermediates.. Journal of Virology 26:357–363
    [Google Scholar]
  21. FitzGerald DJP, Padmanabhan R, Pastan I, Willingham MC. 1983; Adenovirus-induced release of epidermal growth factor and Pseudomonas toxin into the cytosol of KB ceils during receptor-mediated endocytosis.. Cell 32:607–617
    [Google Scholar]
  22. Fox G, Parry NR, Barnett PV, McGinn B, Rowlands DJ, Brown F. 1989; The cell attachment site on foot-and-mouth disease virus includes the amino acid sequence RGD (arginine-glycine-aspartic acid).. Journal of General Virology 70:625–637
    [Google Scholar]
  23. GarciaPardo A, Wayner EA, Carter WG, Ferreira OC. 1990; Human B lymphocytes define an alternative mechanism of adhesion to fibronectin: the interaction of the α4-/βl integrin with the LHGPEILDVPST sequence of the type III connecting segment is sufficient to promote cell attachment.. Journal of Immunology 144:3361–3366
    [Google Scholar]
  24. Greve JM, Davis G, Meyer AM, Forte CP, Yost SC, Marlor CW, Kamarck ME, McClelland A. 1989; The major human rhinovirus receptor is ICAM-1.. Cell 56:839–847
    [Google Scholar]
  25. Guan JL, Hynes RO. 1990; Lymphoid cells recognize an alternatively spliced segment of fibronectin via the integrin receptor α4/βl.. Cell 60:53–61
    [Google Scholar]
  26. Hennache B, Boulanger P. 1977; Biochemical study of KB cell receptor for adenovirus.. Biochemical Journal 166:237–247
    [Google Scholar]
  27. Hennache B, Torpier G, Boulanger P. 1979; Freeze-fracture study of adenovirus-induced KB cell surface alterations.. Experimental Cell Research 124:139–150
    [Google Scholar]
  28. Hennache B, Torpier G, Boulanger P. 1982; Adenovirus adsorption and sterol redistribution in KB cell plasma membrane.. Experimental Cell Research 137:459–463
    [Google Scholar]
  29. Herold BC, WuDunn D, Soltys N, Spear PG. 1991; Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity.. Journal of Virology 65:1090–1098
    [Google Scholar]
  30. Horwitz A, Duggan K, Buck C, Beckerle MC, Burridge K. 1986; Interaction of plasma membrane fibronectin receptor with talin-a transmembrane linkage.. Nature, London 320:531–533
    [Google Scholar]
  31. Hughes RC, Mautner V. 1973; Interaction of adenovirus with host-cell membranes.. In Membrane-mediated Information pp. 104125 Edited by P. Kent.. Lancaster: Medical and Technical Publishing Co.;
    [Google Scholar]
  32. Humphries MJ. 1990; The molecular basis and specificity of integrin-ligand interactions.. Journal of Cell Science 97:585–592
    [Google Scholar]
  33. Hynes RO. 1987; Integrins: a family of cell surface receptors.. Cell 48:549–554
    [Google Scholar]
  34. Isberg RR, Leong JM. 1990; Multiple /β1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells.. Cell 60:861–871
    [Google Scholar]
  35. Laemmli UK. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  36. Leong JM, Fournier RS, Isberg RR. 1990a; Identification of the integrin binding domain of the Yersinia pseudotuberculosis invasin protein.. EMBO Journal 9:1979–1989
    [Google Scholar]
  37. Leong JM, Fournier RS, Isberg RR. 1990b; Mapping and topographic localization of epitopes of the Yersinia pseudotuberculosis invasin protein.. Infection and Immunity 59:3424–3433
    [Google Scholar]
  38. Lonberg-Holm K, Crowell RL, Philipson L. 1976; Unrelated animal viruses share receptors.. Nature, London 259:679–681
    [Google Scholar]
  39. Mapoles JE, Rrah DL, Crowell RL. 1985; Purification of a HeLa cell receptor protein for the group B coxsackieviruses.. Journal of Virology 55:560–566
    [Google Scholar]
  40. Mbida AD, Pozzetto B, Gaudin OG, Grattard F, LeBihan JC, Akono Y, Ros A. 1992; A 44,000 glycoprotein is involved in the attachment of echovirus-11 onto susceptible cells.. Virology 189:350–353
    [Google Scholar]
  41. Mendelsohn CL, Wimmer E, Racaniello VR. 1989; Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily.. Cell 855:560–865
    [Google Scholar]
  42. Mischak H, Neubauer C, Berger B, Kuechler E, Blaas D. 1988; Detection of the human rhinovirus minor group receptor on renaturing Western blots.. Journal of General Virology 69:2653–2656
    [Google Scholar]
  43. Morgan C, Rosenkranz HS, Mednis B. 1969; Structure and development of viruses as observed in the electron microscope. X. Entry and uncoating of adenovirus.. Journal of Virology 4:777–796
    [Google Scholar]
  44. Neurath AR, Hartzell RW, Rubin BA. 1970; Partial characterization of the complementary sites involved in the reaction between adenovirus type 7 and erythrocyte receptors.. Virology 42:789–793
    [Google Scholar]
  45. Patterson S, Russell WC. 1983; Ultrastructural and immunofluorescence studies of early events in adenovirus-HeLa cell interactions.. Journal of General Virology 64:1091–1099
    [Google Scholar]
  46. Persson R, Svensson U, Everitt E. 1983; Virus receptor interaction in the adenovirus system. II. Capping and cooperative binding of virions on HeLa cells.. Journal of Virology 46:956–963
    [Google Scholar]
  47. Persson R, Svensson U, Everitt E. 1985; Virus receptor interaction in the adenovirus system: characterization of the positive cooperative binding of virions on HeLa cells.. Journal of Virology 54:92–97
    [Google Scholar]
  48. Petterson U. 1984; Structural and non-structural adenovirus proteins. In , pp.. In The Adenoviruses pp. 205–270 Edited by H. S. Ginsberg. New York: Plenum Press;
    [Google Scholar]
  49. Philipson L, Lonberg-Holm K, Petterson U. 1968; Virus-receptor interaction in an adenovirus system.. Journal of Virology 2:1064–1075
    [Google Scholar]
  50. Register RB, Uncapher CR, Naylor AM, Lineberger DW, Colonno RJ. 1991; Human-murine chimeras of ICAM-1 identify amino acid residues critical for rhinovirus and antibody binding.. Journal of Virology 65:6589–6596
    [Google Scholar]
  51. Roberts RJ, Akusjarvi G, Alestrom P, Gelinas RE, Gingeras TR, Sciaky D, Pettersson U. 1986; A consensus sequence for the adenovirus 2 genome. In Adenovirus DNA: The Viral Genome and Its Expression pp. 1–51 Edited by W. Doerfler.. Boston: Martinus Nijhoff;
    [Google Scholar]
  52. Yoshimura K, Trapnell B, Yoneyama K, Rosenthal E, Dalemans W, Fukayama M, Bargon J, Stier LE, Stratford-Perricaudet L, Perricaudet M, Guggino WB, Pavirani A, Lecocq JP, Crystal RG. 1992; In vivo transfer of the human cystic fibrosis transmembrane conductance regulator gene to the airway epithelium.. Cell 68:143–155
    [Google Scholar]
  53. Ruoshlahti E. 1988; Fibronectin and its receptors.. Annual Review of Biochemistry 57:375–413
    [Google Scholar]
  54. Ruoshlahti E, Pierschbacher MD. 1987; New perspectives in cell adhesion: RGD and integrins.. Science 238:491–497
    [Google Scholar]
  55. Ruoshlahti E, Pierschbacher MD. 1987; New perspectives in cell adhesion: RGD and integrins.. Science 238:491–497
    [Google Scholar]
  56. Scatchard G. 1949; The attraction of proteins for small molecules and ions.. Annals of the New York Academy of Sciences 51:660–672
    [Google Scholar]
  57. Seth P, FitzGerald D, Ginsberg HS, Willingham M, Pastan I. 1984; Evidence that the penton base of adenovirus is involved in potentiation of toxicity of Pseudomonas exotoxin conjugated to epidermal growth factor.. Molecular and Cellular Biology 4:1528–1533
    [Google Scholar]
  58. Shieh MT, WuDunn D, Montgomery RI, Esko JD, Spear PG. 1992; Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans.. Journal of Cell Biology 116:1273–1279
    [Google Scholar]
  59. Shimuzu Y, Shaw S. 1991; Lymphocyte interactions with extracellular matrix.. Federation of American Societies for Experimental Biology Journal 5:2292–2299
    [Google Scholar]
  60. Silver L, Anderson CW. 1988; Interaction of human adenovirus serotype 2 with human lymphoid cells.. Virology 165:377–387
    [Google Scholar]
  61. Springer TA. 1990a; Adhesion receptors of the immune system.. Nature, London 346:425–434
    [Google Scholar]
  62. Springer TA. 1990b; The sensation and regulation of interactions with the extracellular environment: the cell biology of lymphocyte adhesion receptors.. Annual Review of Cell Biology 6:359–402
    [Google Scholar]
  63. Staquet MJ, Levarlet B, Dezutter-Dambuyant C, Schmitt D, Thivolet J. 1990; Identification of specific human epithelial cell integrin receptors as VLA proteins.. Experimental Cell Research 187:277–283
    [Google Scholar]
  64. Staunton DE, Merluzzi VJ, Rothlein R, Barton R, Marlin SD, Springer TA. 1989; A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses.. Cell 56:849–853
    [Google Scholar]
  65. Svensson U. 1985; Role of vesicles during adenovirus 2 internalization into HeLa cells.. Journal of Virology 55:442–449
    [Google Scholar]
  66. Svensson U, Persson R. 1984; Entry of adenovirus 2 into HeLa cells.. Journal of Virology 51:687–694
    [Google Scholar]
  67. Svensson U, Persson R, Everitt E. 1981; Virus-receptor interaction in the adenovirus system. I. Identification of virion attachment proteins of the HeLa cell plasma membrane.. Journal of Virology 38:70–81
    [Google Scholar]
  68. Tamkun JW, DeSimone DW, Fonda D, Patel RS, Buck C, Horowitz AF, Hynes RO. 1986; Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin.. Cell 46:271–282
    [Google Scholar]
  69. Uncapher C, DeWitt CM, Colono RJ. 1991; The major and minor group receptor families contain all but one human rhinovirus serotype.. Virology 180:814–817
    [Google Scholar]
  70. Wagner E, Zatloukal K, Cotten M, Kirlappos H, Mechtler K, Curiel DT, Birnsteil ML. 1992; Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.. Proceedings of the National Academy of Sciences, U.S.A. 89:6099–6103
    [Google Scholar]
  71. White JM, Littman DR. 1989; Viral receptors of the immunoglobulin superfamily.. Cell 56:725–728
    [Google Scholar]
  72. Woods A, Johansson S, Hook M. 1988; Fibronectin fibril formation involves cell interactions with two fibronectin domains.. Experimental Cell Research 177:272–283
    [Google Scholar]
  73. Yamada KM. 1989; Fibronectins: structure, functions and receptors.. Current Opinion in Cell Biology 1:956–963
    [Google Scholar]
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