The active adenovirus protease is the intact L3 23K protein Free

Abstract

The L3 23K protein was isolated from adenovirus type 2 and shown to cleave purified substrates, confirming that this protein is the adenovirus protease. Separate antisera, prepared against the amino- and carboxyterminal regions of the 23K protein react with active protease, demonstrating that, contrary to previous reports, zymogen activation is not involved in the regulation of this enzyme. Molecular exclusion chromatography indicated that the protease is active as a monomer. Purified protease was shown to be inhibited by Zn and Cu and by some, but not all, recognized cysteine protease inhibitors, indicating participation of a thiol group and providing additional support to the suggestion that regulation of the enzyme involves a form of thiol-disulphide interchange.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-7-1415
1993-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/7/JV0740071415.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-7-1415&mimeType=html&fmt=ahah

References

  1. Abrahamson M, Dalboge H, Olaffson I, Carlsen S, Grubb A. 1988; Efficient production of native, biologically active human cystatin-c by Escherichia coli.. FEBS Letters 236:14–18
    [Google Scholar]
  2. Anderson CW, Baum PR, Gesteland RF. 1973; Processing of adenovirus 2 induced proteins.. Journal of Virology 12:241–252
    [Google Scholar]
  3. Boudin MLD, Halluin JC, Cousin C, Boulanger P. 198O; Human adenovirus type 2 protein Ilia. II Maturation and encapsidation.. Virology 101:144–156
    [Google Scholar]
  4. Butterworth BE, Korant BD. 1974; Characterization of the large picornaviral peptides produced in the presence of zinc ion.. Journal of Virology 14:282–291
    [Google Scholar]
  5. Cai F, Tang D, Weber JM. 1992; Primary structure of the murine adenovirus type 1 proteinase.. Biochimica et biophysica acta 1129:339–341
    [Google Scholar]
  6. Chatterjee PK, Flint SJ. 1987; Adenovirus type 2 endo-peptidase: an unusual phosphoprotein enzyme matured by autocatalysis.. Proceedings of the National Academy of Sciences, U.S.A. 84:714–718
    [Google Scholar]
  7. Hannan C, Raptis LH, Dery CV, Weber J. 1983; Biological and structural studies with adenovirus type 2 temperature-sensitive mutant defective for uncoating.. Intervirology 19:213–223
    [Google Scholar]
  8. Houde A, Weber LM. 1990; Adenovirus proteinases: comparison of amino acid sequences and expression of the cloned cDNA in Escherichica coli.. Gene 88:269–273
    [Google Scholar]
  9. Karlstrom AR, Levine RL. 1991; Copper inhibits the protease from human immunodeficiency virus 1 by both cysteine dependent and cysteine independent mechanisms.. Gene 88:5552–5556
    [Google Scholar]
  10. Orr DC, Long AC, Kay J, Dunn BM, Cameron JM. 1989; Hydrolysis of a series of synthetic peptide substrates by the human rhinovirus 14 3C proteinase, cloned and expressed in Escherichia coli.. Journal of General 70:2931–2942
    [Google Scholar]
  11. Russell WS, Blair GE. 1977; Polypeptide phosphorylation in adenovirus-infected cells.. Journal of General Virology 34:19–35
    [Google Scholar]
  12. Scheidtmann KH. 1989; Immunological detection of proteins of known sequence.. In Protein Structure: A Practical ApproachEdited by Creighton TE. Oxford & Washington, D.C: IRL Press; pp 93–115
    [Google Scholar]
  13. Smith DB, Johnson KS. 1988; Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S′-transferase.. Gene 67:31–10
    [Google Scholar]
  14. Tremblay ML, Dery CV, Talbot BG. 1983; In vitro cleavage specificity of the adenovirus type 2 proteinase.. Biochimica et biophysica acta 743:239–245
    [Google Scholar]
  15. Weber J. 1976; Genetic analysis of adenovirus type 2. Ill Temperature sensitivity of processing of viral proteins.. Journal of Virology 17:462–471
    [Google Scholar]
  16. Webster A, Russell S, Talbot P, Russell WC, Kemp GD. 1989a; Characterization of the adenovirus proteinase: substrate specificity.. Journal of General Virology 70:3225–3234
    [Google Scholar]
  17. Webster A, Russell WC, Kemp GD. 1989b; Characterization of the adenovirus proteinase: development and use of a specific peptide assay.. Journal of General Virology 70:3215–3223
    [Google Scholar]
  18. Webster A, Hay RT, Kemp GD. 1993; The adenovirus protease is activated by a virus coded disulphide linked peptide.. Cell 72:97–104
    [Google Scholar]
  19. Yeh-Kai L, Akusjarvi G, Alestrom P, Pettersson U, Tremblay M, Weber J. 1983; Genetic identification of an endoproteinase encoded by the adenovirus genome.. Journal of Molecular Biology 167:217–222
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-7-1415
Loading
/content/journal/jgv/10.1099/0022-1317-74-7-1415
Loading

Data & Media loading...

Most cited Most Cited RSS feed