1887

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Volume 74, Issue 7

A mutational analysis of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein Free

Abstract

The herpes simplex virus type 1 origin-binding protein is encoded by gene . We previously described a plasmid, pB1, which encodes a fusion protein containing only the C-terminal 317 amino acids of the UL9 polypeptide and showed that this product retains sequence-specific DNA-binding ability. Two series of pB1 mutants have now been constructed and the polypeptides were tested for origin-binding activity. Using C-terminal truncations, we show that the C-terminal 34 amino acids of UL9 are dispensable for binding and that essential residues lie between positions 801 and 818. Analysis of a series of mutants containing insertions of four amino acids at various positions identified regions of the DNA-binding domain in which alterations either abolished or had relatively little effect upon binding activity. Two mutants which were intermediate in their binding activities also exhibited temperature- or sequence-specific effects.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1993
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1993-07-01
2024-04-19
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References

  1. Baumann RP, Yalamanchili VRR, O’Callaghan DJ. 1989; Functional mapping and DNA sequence of an equine herpesvirus 1 origin of replication.. Journal of Virology 63:1275–1283
     [Google Scholar]
  2. Brennan RG, Matthews BW. 1989; The helix-turn-helix DNA binding motif.. Journal of Biological Chemistry 264:1903–1906
     [Google Scholar]
  3. Bruckner RC, Crute JJ, Dodson MS, Lehman IR. 1991; The herpes simplex virus 1 origin binding protein: a DNA helicase.. Journal of Biological Chemistry 266:2669–2674
     [Google Scholar]
  4. Challberg MD. 1991; Herpes simplex virus DNA replication.. Seminars in Virology 2:247–256
     [Google Scholar]
  5. Chou PY, Fasman GD. 1978; Prediction of the secondary structure of proteins from their amino acid sequence.. Advances in Enzymology 47:45–148
     [Google Scholar]
  6. Davison AJ, Scott JE. 1986; The complete DNA sequence of varicella-zoster virus.. Journal of General Virology 67:1759–1816
     [Google Scholar]
  7. Deb S, Deb SP. 1989; Analysis of Ori-S sequence of HSV-1: identification of one functional DNA binding domain.. Nucleic Acids Research 17:2733–2752
     [Google Scholar]
  8. Deb1 S, Deb SP. 1991; A 269-amino-acid segment with a pseudoleucine zipper and a helix-turn-helix motif codes for the sequence-specific DNA-binding domain of herpes simplex virus type 1 originbinding protein.. Journal of Virology 65:2829–2838
     [Google Scholar]
  9. Dodd IB, Egan JB. 1987; Systematic method for the detection of potential X Cro-like DNA-binding regions in proteins.. Journal of Molecular Biology 194:557–564
     [Google Scholar]
  10. Elias P, Lehman IR. 1988; Interaction of origin binding protein with an origin of replication of herpes simplex virus 1.. Proceedings of the National Academy of Sciences, U.S.A. 85:2959–2963
     [Google Scholar]
  11. Elias P, Gustafsson CM, Hammarsten O. 1990; The origin binding protein of herpes simplex virus 1 binds cooperatively to the viral origin of replication oris.. Journal of Biological Chemistry 265:17167–17173
     [Google Scholar]
  12. Elias P, Gustafsson CM, Hammarsten O, Stow ND. 1992; Structural elements required for the cooperative binding of the herpes simplex virus origin binding protein to oriS reside in the N-terminal part of the protein.. Journal of Biological Chemistry 267:17424–17429
     [Google Scholar]
  13. Fierer DS, Challberg MD. 1992; Purification and characterization of UL9, the herpes simplex virus type 1 originbinding protein.. Journal of Virology 66:3986–3995
     [Google Scholar]
  14. Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM. 1989; Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.. Nucleic Acids Research 17:4713–1730
     [Google Scholar]
  15. Hanahan D. 1985; Techniques for transformation of E. coli.. In DNA Cloning: A Practical Approach vol 1 pp. 109–135Edited by Glover DM. Oxford: IRL Press;
     [Google Scholar]
  16. Hazuda DJ, Perry HC, McClements WL. 1992; Cooperative interactions between replication origin-bound molecules of herpes simplex virus origin-binding protein are mediated via the amino terminus of the protein.. Journal of Biological Chemistry 267:14309–14315
     [Google Scholar]
  17. Hernandez TR, Dutch RE, Lehman IR, Gustafsson C, Elias P. 1991; Mutations in a herpes simplex virus type 1 origin that inhibit interaction with origin-binding protein also inhibit DNA replication.. Journal of Virology 65:1649–1652
     [Google Scholar]
  18. Klupp BG, Kern H, Mettenleiter TC. 1992; The virulencedetermining genomic BamHl fragment 4 of pseudorabies virus contains genes corresponding to the UL15 (partial), UL18, UL19, UL20 and UL21 genes of herpes simplex virus and a putative origin of replication. . Virology 191:900–908
     [Google Scholar]
  19. Koff A, Tegtmeyer P. 1988; Characterization of major recognition sequences for a herpes simplex virus type 1 originbinding protein. Journal of Virology 62:4096–4103
     [Google Scholar]
  20. Koff A, Schwedes JF, Tegtmeyer P. 1991; Herpes simplex virus origin-binding protein (UL9) loops and distorts the viral replication origin.. Journal of Virology 65:3284–3292
     [Google Scholar]
  21. Lockshon D, Galloway DA. 1988; Sequence and structural requirements of a herpes simplex viral DNA replication origin.. Molecular and Cellular Biology 8:4018–4027
     [Google Scholar]
  22. McGeoch DJ, Dalrymple MA, Davison AJ, Dolan A, Frame MC, McNab D, Perry LJ, Scott JE, Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.. Journal of General Virology 69:1531–1574
     [Google Scholar]
  23. Martinez R, Shao L, Weller SK. 1992; The conserved helicase motifs of the herpes simplex virus type 1 origin-binding protein UL9 are important for function.. Journal of Virology 66:6735–6746
     [Google Scholar]
  24. Nicolson L, Cullinane AA, Onions DE. 1990; The nucleotide sequence of an equine herpesvirus 4 gene homologue of the herpes simplex virus 1 glycoprotein H gene.. Journal of General Virology 71:1793–1800
     [Google Scholar]
  25. Nilsson B, Abrahmsen L, Uhlen M. 1985; Immobilization and purification of enzymes with staphylococcal Protein A gene fusion vectors.. EMBO Journal 4:1075–1080
     [Google Scholar]
  26. Olivo PD, Nelson NJ, Challberg MD. 1988; Herpes simplex virus DNA replication: the UL9 gene encodes an originbinding protein. . Proceedings of the National Academy of Sciences, U.S.A. 85:5414–5418
     [Google Scholar]
  27. Rabkin SD, Hanlon B. 1991); Nucleoprotein complex formed between herpes simplex virus UL9 protein and the origin of DNA replication: inter- and intramolecular interactions.. Proceedings of the National Academy of Sciences, U.S.A. 88:10946–10950
     [Google Scholar]
  28. Robertson GR, Scott NA, Miller JM, Sabine M, Zheng M, Bell CW, Whalley JM. 1991; Sequence characteristics of a gene in equine herpesvirus 1 homologous to glycoprotein H of herpes simplex virus.. DNA Sequence 1:241–249
     [Google Scholar]
  29. Stow ND. 1992; Herpes simplex virus type 1 origin-dependent DNA replication in insect cells using recombinant baculoviruses.. Journal of General Virology 13:313–321
     [Google Scholar]
  30. Stow ND, Davison AJ. 1986; Identification of a varicella-zoster virus origin of DNA replication and its activation by herpes simplex virus type 1 gene products.. Journal of General Virology 67:1613–1623
     [Google Scholar]
  31. Stow ND, McMonagle EC. 1983; Characterization of the TRS/IRS origin of DNA replication of herpes simplex virus type 1.. Virology 130:427–438
     [Google Scholar]
  32. Stow ND, Weir HM, Stow EC. 1990; Analysis of the binding sites for the varicella-zoster virus gene 51 product within the viral origin of DNA replication.. Virology 111:570–577
     [Google Scholar]
  33. Telford EAR, Watson MS, McBride K, Davison AJ. 1992; The DNA sequence of equine herpesvirus-1.. Virology 189:304–316
     [Google Scholar]
  34. Towbin H, Staehelin T, Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proceedings of the National Academy of Sciences U.S.A. 76:4350–4354
     [Google Scholar]
  35. Weir HM. 1990 Replication of herpes simplex virus DNA: study of an origin binding protein Ph.D. thesis: University of Glasgow;
     [Google Scholar]
  36. Weir1 HM, Stow ND. 1990; Two binding sites for the herpes simplex virus type 1 UL9 protein are required for efficient activity of the oris replication origin.. Journal of General Virology 71:1379–1385
     [Google Scholar]
  37. Weir HM, Calder JM., Stow ND. 1989; Binding of the herpes simplex virus type 1 UL9 gene product to an origin of viral DNA replication.. Nucleic Acids Research 17:1409–1425
     [Google Scholar]
  38. Weller SK, Spadaro A, Schaffer JE, Murray AW, Maxam AM, Schaffer PA. 1985; Cloning, sequencing, and functional analysis of oriL, a herpes simplex virus type 1 origin of DNA synthesis.. Molecular and Cellular Biology 5:930–942
     [Google Scholar]
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A mutational analysis of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein
J. Gen. Virol. 74, 1349 (1993); https://doi.org/10.1099/0022-1317-74-7-1349
/content/journal/jgv/10.1099/0022-1317-74-7-1349
/content/journal/jgv/10.1099/0022-1317-74-7-1349
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