Transgenic plants producing the 30K temperature-sensitive transport protein (TP) of tobacco mosaic virus (TMV) mutant Ni2519 (affecting cell-to-cell transport) were found to: (i) be susceptible to wild-type TMV U1 at 24 °C (a permissive temperature for Ni2519 TP), (ii) acquire a certain level of resistance to TMV U1 accumulation when maintained at 33 °C (a non-permissive temperature for Ni2519 TP) and (iii) lose the resistance to wild-type TMV after their transfer from 33 °C to 24 °C. It is suggested that reversible temperature-dependent conformational changes in Ni2519 TP are responsible for these phenomena and that production of a TP which is only partially functional in transgenic plants confers on these plants a resistance to the virus owing to reduction of the level of cell-to-cell transport. Transgenic tobacco plants producing the 32K TP of brome mosaic virus (BMV) acquired resistance to TMV U1 suggesting that BMV TP is partially functional in tobacco plants.
AtabekovJ. G.,
TalianskyM. E.1990; Expression of a plant virus-coded transport function of different viral genomes. Advances in Virus Research 38:201–248
AtkinsD.,
HullR.,
WellsB.,
RobertsK.,
MooreP.,
BeachyR. N.1991; The tobacco mosaic virus 30K movement protein in transgenic tobacco plants is localized to plasmodesmata. Journal of General Virology 72:209–211
BernaA.,
Godefroy-ColburnT.,
Stussi-GaraudC.1985; Preparation of an antiserum against an in vitro translation product of alfalfa mosaic virus RNA 3. Journal of General Virology 66:1669–1678
BernaA.,
GafnyR.,
WolfS.,
LucasW. J.,
HoltC. A.,
BeachyR. N.1991; The TMV movement protein: role of the C-terminal 73 amino acids in subcellular localization and function. Virology 182:682–689
CalderV. L.,
PalukaitisP.1992; Nucleotide sequence analysis of the movement genes of resistance breaking strains of tomato mosaic virus. Journal of General Virology 73:165–168
CitovskyV.,
KnorrD.,
SchusterG.,
ZambryskiP.1990; The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding protein. Cell 60:637–647
CitovskyV.,
WongM. L.,
ShawA. L.,
PrasadB. V.,
ZambryskiP.1992; Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids. Plant Cell A397–411
ClarkM. F.,
AdamsA. N.1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
DaubertS.1991; Molecular determinants of plant-virus interaction. In Molecular Signals in Plant-Microbe Communication pp. 423–443 Edited by
VernaD. P.
Boca Raton: CRC Press;
De JongW.,
AhlquistP.1992; A hybrid plant RNA virus made by transferring the noncapsid movement protein from a rod-shaped to an icosahedral virus is competent for systemic infection. Proceedings of the National Academy of Sciences, U.S.A. 89:6808–6812
DeomC. M.,
SchubertK.,
WolfS.,
HoltC. A.,
LucasW. I.,
BeachyR. N.1990; Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proceedings of the National Academy of Sciences, U.S.A. 87:3284–3288
DeomC. M.,
WolfS.,
HoltC. A.,
LucasW. J.,
BeachyR. N.1991; Altered function of the tobacco mosaic virus movement protein in a hypersensitive host. Virology 180:251–256
DingB.,
HaudenshieldJ. S.,
HullR. J.,
WolfS.,
BeachyR.,
LucasW. J.1992; Secondary plasmodesmata are specific sites of localization of the tobacco mosaic movement protein in transgenic plants. Plant Cell 4:915–928
Godefroy-ColburnT.,
ErnyC.,
SchoumacherF.,
BernaA.,
GadeyM.-J.,
Stussi-GaraudC.1990; Cell to cell movement of plant viruses. In Plant Molecular Biology2 pp. 35–48 Edited by
HerrmannR. G.
New York: Plenum Press;
GoeletP.,
LomonossoffG.,
ButlerP. J. G.,
AkamM.,
GattM.,
KarnJ.1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U.S.A. 79:5818–5822
HamiltonR. I.,
NicolsC.1977; The influence of bromegrass mosaic virus on the replication of tobacco mosaic virus in Hordeum vulgare. Phytopathology 67:484–489
HorschR. B.,
FryJ. E.,
HoffmanN. L.,
EichholizD.,
RogersS. G.,
FraleyR. T.1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
JacksonA. O.,
PettyI. T. D.,
JonesR. W.,
EdwardsM. C.,
FrenchR.1991; Analysis of barley stripe mosaic virus pathogenicity. Seminars in Virology 2:107–119
MalyshenkoS. L.,
KaplanI. B.,
MushegianA. R.,
TalianskyM. E.,
AtabekovJ. G.1985; Stimulation of potato virus X reproduction in the cells of susceptible and resistant plants. Izvestia Academii nauk SSSR Seria Biologicheskaya 3:339–343 (in Russian)
MalyshenkoS. L.,
TalianskyM. E.,
KondakovaO. A.,
UlanovaE. F.,
AtabekovJ. G.1987; Plant virus-specific transport function is a factor controlling virus host range. Izvestia Academii nauk SSSR Seria Biologicheskaya 5:680–685 (in Russian)
MalyshenkoS. L.,
LapchicL. G.,
KondakovaO. A.,
KuznetzovaL. L.,
TalianskyM. E.,
AtabekovJ. G.1988; Red clover mottle comovirus B-RNA spreads between cells in tobamovirus-infected tissues. Journal of General Virology 69:407–412
MalyshenkoS. L.,
KondakovaO. A.,
TalianskyM. E.,
AtabekovJ. G.1989; Plant virus transport function: complementation by helper viruses is non-specific. Journal of General Virology 70:2751–2757
MeshiT.,
WatanabeY.,
SaitoT.,
SugimotoA.,
MaedaT.,
OkadaY.1987; Function of the 30kd protein tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication. EMBO Journal 6:2557–2563
MorozovS. Yu.,
MiroschnichenkoN. A.,
SolovyevA. G.,
ZeleninaD. A.,
FedorkinO. N.,
LukashevaL. I.,
AtabekovJ. G.1990; Expression of RNA transcripts of PVX full-length and subgenomic cDNAs. Biochimie 72:677–684
MoserO.,
GageyM.-J.,
Godefroy-ColburnT.,
Stussi-GaraudC.,
Ellwart-TschürtzM.,
NitschkoH.,
MundryK.-W.1988; The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. Journal of General Virology 69:1367–1373
NejidatA.,
BeachyR. N.1989; Decreased levels of TMV coat protein in transgenic tobacco plants and elevated temperatures reduce resistance to TMV infection. Virology 173:531–538
OhnoT.,
TakamatsuN.,
OkadaY.,
NishiguchiM.,
KihoY.1983; Single amino acid substitution in 30K protein of TMV defective in virus transport function. Virology 131:255–258
OhnoT.,
AoyagiM.,
YamanashiY.,
SaitoH.,
IkawaS.,
MeshiT.,
OkadaY.1984; Nucleotide sequence of tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Journal of Biochemistry, Tokyo 96:1915–1923
OsmanT. A. M.,
HayesR. J.,
BuckK. W.1992; Cooperative binding of the red clover necrotic mosaic virus movement protein to single-stranded nucleic acids. Journal of General Virology 73:223–227
SakaiF.,
DawsonJ. R. O.,
WattsJ. W.1983; Interference in infections of tobacco protoplasts with two bromoviruses. Journal of General Virology 64:1347–1354
TalianskyM. E.,
MalyshenkoS. L.,
KaplanI. B.,
KondakovaO. A.,
AtabekovJ. G.1991; Functional analysis of tobacco plants transgenic in ts and tr 30K transport gene of TMV. Abstracts of the FEBS Advanced Course: The Plant Virus Genome Structure and Expression Riga, U.S.S.R. p. 10
TalianskyM. E.,
MalyshenkoS. L.,
KaplanI. B.,
KondakovaO. A.,
AtabekovJ. G.1992; Production of the tobacco mosaic virus (TMV) transport protein in transgenic plants is essential but insufficient for complementing foreign virus transport: a need for the full-length TMV genome or some other TMV-encoded product. Journal of General Virology 73:471–474
TomeniusK.,
ClaphamD.,
MeshiT.1987; Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus. Virology 160:363–371
TopferR.,
MatzeitV.,
GronenbornB.,
SchellJ.,
SteinbissH.-H.1987; A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Research 15:5890
WolfS.,
DeomC. M.,
BeachyR. N.,
LucasW. L.1989; Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246:337–339
WolfS.,
DeomC. M.,
BeachyR. N.,
LucasW. L.1991; Plasmodesmatal function probed using transgenic tobacco plants that express a virus movement protein. Plant Cell 3:593–604
ZimmernD.,
HunterT.1983; Point mutation in the 30kD open reading frame of TMV implicated in temperature sensitive assembly and local lesion spreading of mutant Ni2519. EMBO Journal 2:1893–1990