Reduction of tobacco mosaic virus accumulation in transgenic plants producing non-functional viral transport proteins Free

Abstract

Transgenic plants producing the 30K temperature-sensitive transport protein (TP) of tobacco mosaic virus (TMV) mutant Ni2519 (affecting cell-to-cell transport) were found to: (i) be susceptible to wild-type TMV U1 at 24 °C (a permissive temperature for Ni2519 TP), (ii) acquire a certain level of resistance to TMV U1 accumulation when maintained at 33 °C (a non-permissive temperature for Ni2519 TP) and (iii) lose the resistance to wild-type TMV after their transfer from 33 °C to 24 °C. It is suggested that reversible temperature-dependent conformational changes in Ni2519 TP are responsible for these phenomena and that production of a TP which is only partially functional in transgenic plants confers on these plants a resistance to the virus owing to reduction of the level of cell-to-cell transport. Transgenic tobacco plants producing the 32K TP of brome mosaic virus (BMV) acquired resistance to TMV U1 suggesting that BMV TP is partially functional in tobacco plants.

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1993-06-01
2024-03-28
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References

  1. Ahlquist P., Luckow V., Kaesberg P. 1981; Complete nucleotide sequence of brome mosaic virus RNA3. Journal of Molecular Biology 153:23–28
    [Google Scholar]
  2. Asselin A., Zaitlin M. 1978; Characterization of a second protein associated with virions of tobacco mosaic virus. Virology 91:173–181
    [Google Scholar]
  3. Atabekov J. G., Dorokhov Y. L. 1984; Plant virus-specific transport function and resistance of plants to viruses. Advances in Virus Research 29:313–364
    [Google Scholar]
  4. Atabekov J. G., Taliansky M. E. 1990; Expression of a plant virus-coded transport function of different viral genomes. Advances in Virus Research 38:201–248
    [Google Scholar]
  5. Atkins D., Hull R., Wells B., Roberts K., Moore P., Beachy R. N. 1991; The tobacco mosaic virus 30K movement protein in transgenic tobacco plants is localized to plasmodesmata. Journal of General Virology 72:209–211
    [Google Scholar]
  6. Berna A., Godefroy-Colburn T., Stussi-Garaud C. 1985; Preparation of an antiserum against an in vitro translation product of alfalfa mosaic virus RNA 3. Journal of General Virology 66:1669–1678
    [Google Scholar]
  7. Berna A., Gafny R., Wolf S., Lucas W. J., Holt C. A., Beachy R. N. 1991; The TMV movement protein: role of the C-terminal 73 amino acids in subcellular localization and function. Virology 182:682–689
    [Google Scholar]
  8. Bevan M. 1984; Binary Agrobacterium vectors for plant transformation. Nucleic Acids Research 12:8711–8721
    [Google Scholar]
  9. Calder V. L., Palukaitis P. 1992; Nucleotide sequence analysis of the movement genes of resistance breaking strains of tomato mosaic virus. Journal of General Virology 73:165–168
    [Google Scholar]
  10. Citovsky V., Zambryski P. 1991; How do plant virus nucleic acids move through intercellular connections?. Bio Essays 13:373–379
    [Google Scholar]
  11. Citovsky V., Knorr D., Schuster G., Zambryski P. 1990; The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding protein. Cell 60:637–647
    [Google Scholar]
  12. Citovsky V., Wong M. L., Shaw A. L., Prasad B. V., Zambryski P. 1992; Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids. Plant Cell A397–411
    [Google Scholar]
  13. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  14. Daubert S. 1991; Molecular determinants of plant-virus interaction. In Molecular Signals in Plant-Microbe Communication pp. 423–443 Edited by Verna D. P. Boca Raton: CRC Press;
    [Google Scholar]
  15. De Jong W., Ahlquist P. 1992; A hybrid plant RNA virus made by transferring the noncapsid movement protein from a rod-shaped to an icosahedral virus is competent for systemic infection. Proceedings of the National Academy of Sciences, U.S.A. 89:6808–6812
    [Google Scholar]
  16. Deom C. M., Oliver M. I., Beachy R. N. 1987; The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237:389–394
    [Google Scholar]
  17. Deom C. M., Schubert K., Wolf S., Holt C. A., Lucas W. I., Beachy R. N. 1990; Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proceedings of the National Academy of Sciences, U.S.A. 87:3284–3288
    [Google Scholar]
  18. Deom C. M., Wolf S., Holt C. A., Lucas W. J., Beachy R. N. 1991; Altered function of the tobacco mosaic virus movement protein in a hypersensitive host. Virology 180:251–256
    [Google Scholar]
  19. Deom C. M., Lapidot M., Beachy R. 1992; Plant virus movement proteins. Cell 69:221–224
    [Google Scholar]
  20. Ding B., Haudenshield J. S., Hull R. J., Wolf S., Beachy R., Lucas W. J. 1992; Secondary plasmodesmata are specific sites of localization of the tobacco mosaic movement protein in transgenic plants. Plant Cell 4:915–928
    [Google Scholar]
  21. Godefroy-Colburn T., Erny C., Schoumacher F., Berna A., Gadey M.-J., Stussi-Garaud C. 1990; Cell to cell movement of plant viruses. In Plant Molecular Biology 2 pp. 35–48 Edited by Herrmann R. G. New York: Plenum Press;
    [Google Scholar]
  22. Goelet P., Lomonossoff G., Butler P. J. G., Akam M., Gatt M., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U.S.A. 79:5818–5822
    [Google Scholar]
  23. Hamilton R. I., Nicols C. 1977; The influence of bromegrass mosaic virus on the replication of tobacco mosaic virus in Hordeum vulgare. Phytopathology 67:484–489
    [Google Scholar]
  24. Horsch R. B., Fry J. E., Hoffman N. L., Eichholiz D., Rogers S. G., Fraley R. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
    [Google Scholar]
  25. Hull R. 1989; The movement of viruses in plants. Annual Review of Phytopathology 27:213–240
    [Google Scholar]
  26. Jackson A. O., Petty I. T. D., Jones R. W., Edwards M. C., French R. 1991; Analysis of barley stripe mosaic virus pathogenicity. Seminars in Virology 2:107–119
    [Google Scholar]
  27. Lehto K., Burbrick P., Dawson W. O. 1990; Time course of TMV 30K protein accumulation in intact leaves. Virology 174:290–298
    [Google Scholar]
  28. Malyshenko S. L., Kaplan I. B., Mushegian A. R., Taliansky M. E., Atabekov J. G. 1985; Stimulation of potato virus X reproduction in the cells of susceptible and resistant plants. Izvestia Academii nauk SSSR Seria Biologicheskaya 3:339–343 (in Russian)
    [Google Scholar]
  29. Malyshenko S. L., Taliansky M. E., Kondakova O. A., Ulanova E. F., Atabekov J. G. 1987; Plant virus-specific transport function is a factor controlling virus host range. Izvestia Academii nauk SSSR Seria Biologicheskaya 5:680–685 (in Russian)
    [Google Scholar]
  30. Malyshenko S. L., Lapchic L. G., Kondakova O. A., Kuznetzova L. L., Taliansky M. E., Atabekov J. G. 1988; Red clover mottle comovirus B-RNA spreads between cells in tobamovirus-infected tissues. Journal of General Virology 69:407–412
    [Google Scholar]
  31. Malyshenko S. L., Kondakova O. A., Taliansky M. E., Atabekov J. G. 1989; Plant virus transport function: complementation by helper viruses is non-specific. Journal of General Virology 70:2751–2757
    [Google Scholar]
  32. Maule A. J. 1991; Virus movement in infected plants. CRC Critical Reviews in Plant Sciences 9:457–473
    [Google Scholar]
  33. Meshi T., Watanabe Y., Saito T., Sugimoto A., Maeda T., Okada Y. 1987; Function of the 30kd protein tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication. EMBO Journal 6:2557–2563
    [Google Scholar]
  34. Morozov S. Yu., Miroschnichenko N. A., Solovyev A. G., Zelenina D. A., Fedorkin O. N., Lukasheva L. I., Atabekov J. G. 1990; Expression of RNA transcripts of PVX full-length and subgenomic cDNAs. Biochimie 72:677–684
    [Google Scholar]
  35. Moser O., Gagey M.-J., Godefroy-Colburn T., Stussi-Garaud C., Ellwart-Tschürtz M., Nitschko H., Mundry K.-W. 1988; The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. Journal of General Virology 69:1367–1373
    [Google Scholar]
  36. Nejidat A., Beachy R. N. 1989; Decreased levels of TMV coat protein in transgenic tobacco plants and elevated temperatures reduce resistance to TMV infection. Virology 173:531–538
    [Google Scholar]
  37. Ohno T., Takamatsu N., Okada Y., Nishiguchi M., Kiho Y. 1983; Single amino acid substitution in 30K protein of TMV defective in virus transport function. Virology 131:255–258
    [Google Scholar]
  38. Ohno T., Aoyagi M., Yamanashi Y., Saito H., Ikawa S., Meshi T., Okada Y. 1984; Nucleotide sequence of tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. Journal of Biochemistry, Tokyo 96:1915–1923
    [Google Scholar]
  39. Osman T. A. M., Hayes R. J., Buck K. W. 1992; Cooperative binding of the red clover necrotic mosaic virus movement protein to single-stranded nucleic acids. Journal of General Virology 73:223–227
    [Google Scholar]
  40. Saito T., Imai Y., Meshi T., Okada Y. 1988; Interviral homologies of the 30K proteins of tobamoviruses. Virology 167:653–656
    [Google Scholar]
  41. Sakai F., Dawson J. R. O., Watts J. W. 1983; Interference in infections of tobacco protoplasts with two bromoviruses. Journal of General Virology 64:1347–1354
    [Google Scholar]
  42. Taliansky M. E., Malyshenko S. L., Pshennikova E. S., Atabekov J. G. 1982; Plant virus-specific transport function. II. A factor controlling virus host range. Virology 122:327–331
    [Google Scholar]
  43. Taliansky M. E., Malyshenko S. L., Kaplan I. B., Kondakova O. A., Atabekov J. G. 1991; Functional analysis of tobacco plants transgenic in ts and tr 30K transport gene of TMV. Abstracts of the FEBS Advanced Course: The Plant Virus Genome Structure and Expression Riga, U.S.S.R. p. 10
    [Google Scholar]
  44. Taliansky M. E., Malyshenko S. L., Kaplan I. B., Kondakova O. A., Atabekov J. G. 1992; Production of the tobacco mosaic virus (TMV) transport protein in transgenic plants is essential but insufficient for complementing foreign virus transport: a need for the full-length TMV genome or some other TMV-encoded product. Journal of General Virology 73:471–474
    [Google Scholar]
  45. Tomenius K., Clapham D., Meshi T. 1987; Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus. Virology 160:363–371
    [Google Scholar]
  46. Topfer R., Matzeit V., Gronenborn B., Schell J., Steinbiss H.-H. 1987; A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Research 15:5890
    [Google Scholar]
  47. Wolf S., Deom C. M., Beachy R. N., Lucas W. L. 1989; Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246:337–339
    [Google Scholar]
  48. Wolf S., Deom C. M., Beachy R. N., Lucas W. L. 1991; Plasmodesmatal function probed using transgenic tobacco plants that express a virus movement protein. Plant Cell 3:593–604
    [Google Scholar]
  49. Zimmern D., Hunter T. 1983; Point mutation in the 30kD open reading frame of TMV implicated in temperature sensitive assembly and local lesion spreading of mutant Ni2519. EMBO Journal 2:1893–1990
    [Google Scholar]
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