1887

Abstract

Alkali-denatured cauliflower mosaic virus (CaMV) virions incorporated ssDNA, added exogenously, into multimolecular complexes during dialysis against a neutral buffer. CaMV coat protein binding to tracer DNA, assessed by gel electrophoresis and autoradiography, was highly cooperative as judged by the absence of intermediate-sized complexes. The incorporation of labelled II fragments of a plasmid containing CaMV DNA into complexes was prevented by the presence of 0.16 g/l unlabelled calf thymus DNA. Lower concentrations of competitor DNA allowed binding of some II fragments although preventing the binding of others. The self-annealing poly(dI-dC) was much less efficient than calf thymus DNA in preventing the incorporation of fragments into complexes, suggesting a binding preference for ss- over dsDNA. In addition, dsDNA, minimally cross-linked to prevent strand separation, was bound only weakly. End-labelled ssDNA fragments in complexes were partially protected against DNase I. The nucleic acid-binding activity of CaMV coat protein may be responsible for the organization of replication complexes, the precursors to virion particles.

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1993-06-01
2024-04-19
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References

  1. Al Ani R., Pfeiffer P., Lebeurier G., Hirth L. 1979; The structure of cauliflower mosaic virus. 1. pH-induced structural changes. Virology 93:175–187
    [Google Scholar]
  2. Citovsky V., Knorr D., Zambryski P. 1991; Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein. Proceedings of the National Academy of Sciences, U.S.A. 88:2476–2480
    [Google Scholar]
  3. Covey S. N. 1986; Amino acid sequence homology in gag region of reverse transcribing elements and the coat protein gene of cauliflower mosaic virus. Nucleic Acids Research 14:623–633
    [Google Scholar]
  4. Darlix J.-L., Spahr P.-F. 1982; Binding sites of viral protein P19 onto Rous sarcoma virus RNA and possible controls of viral functions. Journal of Molecular Biology 160:147–161
    [Google Scholar]
  5. Fuetterer J., Hohn T. 1987; Involvement of nucleocapsids in reverse transcription: a general phenomenon?. Trends in Biochemical Sciences 12:92–95
    [Google Scholar]
  6. Gaillard C., Strauss F. 1990; Sequence-specific single-strandbinding protein for the simian virus 40 early promoter stimulates transcription in vitro. Journal of Molecular Biology 215:245–255
    [Google Scholar]
  7. Gardner R. C., Shepherd R. J. 1980; A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA. Virology 106:159–161
    [Google Scholar]
  8. Gasparo F. P., Saffran W. A., Cantor C. R., Edelson R. L. 1984; Wavelength dependence for AMT crosslinking of pBR322. Photochemistry and Photobiology 40:215–219
    [Google Scholar]
  9. Giband M., Mesnard J. M., Lebeurier G. 1986; The gene III product (PI5) of cauliflower mosaic virus is a DNA-binding protein while an immunologically related PI 1 polypeptide is associated with virions. EMBO Journal 5:2433–2438
    [Google Scholar]
  10. Hull R., Shepherd R. J., Harvey J. D. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles. Journal of General Virology 31:93–100
    [Google Scholar]
  11. Hussain M. M., Melcher U., Whittle T., Williams A., Brannan C. M., Mitchell E. D. Jr 1987; Replication of cauliflower mosaic virus DNA in leaves and suspension culture protoplasts of cotton. Plant Physiology 83:633–639
    [Google Scholar]
  12. Laquel P., Ziegler V., Hirth L. 1986; The 80K polypeptide associated with the replication complexes of cauliflower mosaic virus is recognized by antibodies to gene V translation product. Journal of General Virology 67:197–201
    [Google Scholar]
  13. Lebeurier G., Hirth L., Hohn T., Hohn B. 1980; Infectivities of native and cloned DNA of cauliflower mosaic virus. Gene 12:139–146
    [Google Scholar]
  14. Lightfoote M. M., Coligan J. E., Folks T. M., Fauci A. S., Martin M. A., Venkatesan S. 1986; Structural characterization of reverse transcriptase and endonuclease polypeptides of the acquired immunodeficiency syndrome retrovirus. Journal of Virology 60:771–775
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Marsh L. E., Guilfoyle T. J. 1987; Cauliflower mosaic virus replication intermediates are encapsidated into virion-like particles. Virology 161:129–137
    [Google Scholar]
  17. Marsh L., Kuzi A., Guilfoyle T. 1985; Identification and characterization of cauliflower mosaic virus replication complexes -analogy to hepatitis B viruses. Virology 143:212–223
    [Google Scholar]
  18. Mazzolini L., Dabos P., Constantin S., Yot P. 1989; Further evidence that viroplasms are the site of cauliflower mosaic virus genome replication by reverse transcription during viral infection. Journal of General Virology 70:3439–3449
    [Google Scholar]
  19. Melcher U., Gardner C. O. Jr, Essenberg R. C. 1981; Clones of cauliflower mosaic virus identified by molecular hybridization in turnip leaves. Plant Molecular Biology 1:63–73
    [Google Scholar]
  20. Menissier J., Laquel P., Lebeurier G., Hirth L. 1984; A DNA polymerase activity is associated with cauliflower mosaic virus. Nucleic Acids Research 12:8769–8778
    [Google Scholar]
  21. Méric C., Spahr P.-F. 1986; Rous sarcoma virus nucleic acidbinding protein pl2 is necessary for viral 70S RNA dimer formation and packaging. Journal of Virology 60:450–459
    [Google Scholar]
  22. Méric C., Darlix J.-L., Spahr P.-F. 1984; It is Rous sarcoma virus protein PI2 and not P19 that binds tightly to Rous sarcoma virus RNA. Journal of Molecular Biology 173:531–538
    [Google Scholar]
  23. Mesnard J.-M., Kirchherr D., Wurch T., Lebeurier G. 1990; The cauliflower mosaic virus gene III product is a non-sequence-specific DNA binding protein. Virology 174:622–624
    [Google Scholar]
  24. Pang P. P., Pruitt R. E., Meyerowitz E. M. 1988; Molecular cloning, genomic organization, expression and evolution of 12S seed storage protein genes of Arabidopsis thaliana. Plant Molecular Biology 11:805–820
    [Google Scholar]
  25. Prats A. C., Sarih L., Gabus C., Litvak S., Keith G., Darlix J. L. 1988; Small finger protein of avian and murine retroviruses has nucleic acid annealing activity and positions the replication primer tRNA onto genomic RNA. EMBO Journal 7:1777–1783
    [Google Scholar]
  26. Schulein M., Burnette W. N., August J. T. 1978; Stoichiometry and specificity of binding of Rauscher oncovirus 10,000-dalton (plO) structural protein to nucleic acids. Journal of Virology 26:54–60
    [Google Scholar]
  27. Sen A., Sherr C. J., Todaro G. J. 1976; Specific binding of the type C viral core protein pl2 with purified viral RNA. Cell 7:21–32
    [Google Scholar]
  28. Shepherd R. J. 1989; Biochemistry of DNA plant viruses. In The Biochemistry of Plants pp. 563–616 Edited by Marcus A. New York: Academic Press;
    [Google Scholar]
  29. Smith B. J., Bailey J. M. 1979; The binding of an avian myeloblastosis virus basic 12,000 dalton protein to nucleic acids. Nucleic Acids Research 7:2055–2072
    [Google Scholar]
  30. Smith D. R., Calvo J. M. 1980; Nucleotide sequence of the E. coli gene coding for dihydrofolate reductase. Nucleic Acids Research 8:2255–2274
    [Google Scholar]
  31. Sun T. J., Melcher U., Essenberg M. 1988; Inactivation of cauliflower mosaic virus by a photoactivatable cotton phytoalexin. Physiological and Molecular Plant Pathology 33:115–126
    [Google Scholar]
  32. Thomas C. M., Hull R., Bryant J. A., Maule A. J. 1985; Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (−) strand viral DNA and reverse transcription of primed RNA templates. Nucleic Acids Research 13:4557–4576
    [Google Scholar]
  33. Vaden V. R., Melcher U. 1990; Recombination sites in cauliflower mosaic virus DNAs: implications for mechanisms of recombination. Virology 177:717–726
    [Google Scholar]
  34. Veronese F. D. M., Copeland T. D., DeVico A. L., Rahman R., Oroszlan S., Gallo R. C., Sarngadharan M. G. 1986; Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV. Science 231:1289–1291
    [Google Scholar]
  35. Zhang X. S., Melcher U. 1989; Competition between isolates and variants of cauliflower mosaic virus in infected turnip plants. Journal of General Virology 70:3427–3437
    [Google Scholar]
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