1887

Abstract

Haemorrhagic fever with renal syndrome (HFRS) is a serious and often fatal disease caused by viruses in the genus of the family . We expressed the entire coding region of the small (S) genome segments of three serologically distinct hantaviruses as soluble proteins in and evaluated the expressed nucleocapsid proteins (NPs) as antigens for diagnosis of HFRS. We also prepared novel diagnostic antigens by expressing truncated genes from which we deleted amino acid coding regions that were highly conserved among the three viruses. These antigens were analysed for their potential to detect and differentiate between antisera to various hantaviruses by ELISA. ELISA results obtained with HFRS patient sera or with sera from naturally or experimentally infected animals indicate that homologous antigens and antisera reacted to high titre. The truncated NPs were more specific than the complete NPs in distinguishing between possible aetiological agents of HFRS. Our findings demonstrate that prokaryotic expression of portions of the NPs of specific hantaviruses can be used to generate, readily and efficiently, large quantities of antigen that is both sensitive and specific in diagnostic assays for HFRS.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-6-1115
1993-06-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/6/JV0740061115.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-6-1115&mimeType=html&fmt=ahah

References

  1. Antic D., Kang C. Y., Spik K., Schmaljohn C. S., Vapalahti O., Vaheri A. 1992; Comparison of the deduced gene products of the L, M and S genome segments of hantaviruses. Virus Research 24:35–46
    [Google Scholar]
  2. Arikawa J., Lapenotiere H. F., Iacono-Connors L., Wang M., Schmaljohn C. S. 1990; Coding properties of the S and the M genome segments of SR-11 virus: comparison to other causative agents of hemorrhagic fever with renal syndrome. Virology 176:114–125
    [Google Scholar]
  3. Brummer-Korvenkontio M., Vaheri A., Hovi T., von Bonsdorff C.-H., Vuorimies J., Manni T., Penttinen K., Oker-Blom N., Lähdevirta J. 1980; Nephropathia epidemica: detection of antigen in bank voles and serological diagnosis of human infection. Journal of Infectious Diseases 141:131–134
    [Google Scholar]
  4. Gott P., Zoller L., Yang S., Stohwasser R., Bautz E. K., Darai G. 1991; Antigenicity of hantavirus nucleocapsid proteins expressed in E. coli. Virus Research 19:1–15
    [Google Scholar]
  5. Horton R. M., Hunt H. D., Ho S. N., Pullen J. K., Pease L. R. 1989; Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61–68
    [Google Scholar]
  6. Kitamura T., Morita C., Komatsu T., Sugiyama K., Arikawa J., Shiga S., Taked H., Akao Y., Imaizumi K., Oya A., Hashimoto N., Urasawa S. 1983; Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system. Japanese Journal of Medical Science and Biology 36:17–25
    [Google Scholar]
  7. LeDuc J. W., Smith G. A., Johnson K. M. 1984; Hantaan-like viruses from domestic rats captured in the United States. American Journal of Tropical Medicine and Hygiene 33:992–998
    [Google Scholar]
  8. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologie agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–302
    [Google Scholar]
  9. Lee H. W., French G. R., Lee P. W., Baek L. J., Tsuchiya K., Foulke R. S. 1981a; Observation on natural and laboratory infection of rodents with the etiological agent of Korean hemorrhagic fever. American Journal of Tropical Medicine and Hygiene 30:447–482
    [Google Scholar]
  10. Lee H. W., Lee P. W., Baek L. J., Song C. K., Seong I. W. 1981b; Intraspecific transmission of Hantaan virus, etiologie agent of Korean hemorrhagic fever, in the rodent Apodemus agrarias. American Journal of Tropical Medicine and Hygiene 30:1106–1112
    [Google Scholar]
  11. Lee H. W., Baek L. J., Johnson K. M. 1982; Isolation of Hantaan virus. The etiologie agents of Korean hemorrhagic fever from wild urban rats. Journal of Infectious Diseases 146:638–644
    [Google Scholar]
  12. Lee P. W., Gibbs C. J., Gajdusek C. J., Yanagihara R. 1985; Serotypic classification of hantaviruses by indirect immuno-fluorescent antibody and plaque reduction neutralization tests. Journal of Clinical Microbiology 22:940–944
    [Google Scholar]
  13. Rossi C. A., Schmaljohn C. S., Meegan J. M., LeDuc J. W. 1990; Diagnostic potential of a baculovirus-expressed nucleocapsid protein for hantaviruses. Archives of Virology Supplement 1:19–28
    [Google Scholar]
  14. Ruo S. L., Sanchez A., Elliott L. H., Brammer L. S., McCormick J. B., Fisher-Hoch S. P. 1991; Monoclonal antibodies to three strains of hantaviruses: Hantaan, R22 and Puumala. Archives of Virology 119:1–11
    [Google Scholar]
  15. Schmaljohn C. S., Hasty S. E., Dalrymple J. M., LeDuc J. W., Lee H. W., von Bonsdorff C. H., Brummer-Korvenkontio M., Vaheri A., Tsai T. F., Regnery H. L., Goldgaber D., Lee P. W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
    [Google Scholar]
  16. Schmaljohn C. S., Jennings G. B., Hay J., Dalrymple J. M. 1986; Coding strategy of the S genome segment of Hantaan virus. Virology 155:633–643
    [Google Scholar]
  17. Schmaljohn C. S., Sugiyama K., Schmaljohn A. L., Bishop D. H. L. 1988a; Baculovirus expression of the small genome segment of Hantaan virus and potential use of the expressed nucleocapsid protein as a diagnostic antigen. Journal of General Virology 69:777–786
    [Google Scholar]
  18. Schmaljohn C. S., Arikawa J., Hasty S. E., Rasmussen L., Lee H. W., Lee P. W., Dalrymple J. M. 1988b; Conservation of antigenic properties and sequences encoding the envelope proteins of prototype Hantaan virus and two virus isolates from Korean haemorrhagic fever patients. Journal of General Virology 69:1949–1955
    [Google Scholar]
  19. Sheshberadaran H., Niklasson B., Tkachenko E. A. 1988; Antigenic relationship between hantaviruses analysed by immunoprecipitation. Journal of General Virology 69:2645–2651
    [Google Scholar]
  20. Song G., Hang C. S., Liao H. X., Fu J. L., Gao G. Z., Qui H. L., Zhang Q. F. 1984; Antigenic differences between viral strains causing classical and mild types of epidemic hemorrhagic fever with renal syndrome in China. Journal of Infectious Diseases 150:889–894
    [Google Scholar]
  21. Stohwasser R., Giebel L. B., Zoller L., Bautz E. K., Darai G. 1990; Molecular characterization of the RNA S segment of nephropathia epidemica virus strain Hallnás Bl. Virology 174:79–86
    [Google Scholar]
  22. Tkachenko E. A., Bashkirtsev V. N., van der Groen G., Dzag-urova T. K., Ivanov A. P., Ryltseva E. V. 1984; Isolation in Vero-E6 cells of hantavirus from Clethrionomys glareolus captured in the Bashkiria area of the USSR. Anuales de la Societe Belge de Medecine Tropicale 64:425–426
    [Google Scholar]
  23. Yanagihara R., Svedmyr A., Amyx H. L., Lee P., Goldgaber D., Gajdusek D. C., Gibbs C. J. Jr, Nystrom K. 1984; Isolation and propagation of nephropathia epidemica virus in bank voles. Scandinavian Journal of Infectious Diseases 16:225–228
    [Google Scholar]
  24. Zoller L., Scholz J., Stohwasser R., Giebel L. B., Sethi K. K., Bautz E. K., Darai G. 1989; Immunoblot analysis of the serological response in hantavirus infections. Journal of Medical Virology 27:231–237
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-6-1115
Loading
/content/journal/jgv/10.1099/0022-1317-74-6-1115
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error