RT Journal Article SR Electronic(1) A1 Martin, P. E. M. A1 McCrae, M. A.YR 1993 T1 Analysis of the stimulation of reporter gene expression by the σ3 protein of reovirus in co-transfected cells JF Journal of General Virology, VO 74 IS 6 SP 1055 OP 1062 DO https://doi.org/10.1099/0022-1317-74-6-1055 PB Microbiology Society, SN 1465-2099, AB The stimulation of reporter gene expression following co-transfection with the S4 gene of mammalian reoviruses was analysed. The σ3 protein of type 3 reovirus gave a five- to eightfold increase in expression of chloramphenicol acetyltransferase and β-galactosidase but this was found to be dependent upon the nature of the promoter being used to drive reporter gene expression. The σ3 protein of reovirus type 1 failed to stimulate reporter gene expression under any of the conditions used. Hybrid constructs between the S4 genes of reoviruses type 1 and 3 were used to map the stimulation characteristic to the carboxy-terminal third of the gene. Analysis of the level of σ3 protein accumulation in transfected cells showed that the reovirus type 3 protein accumulated to a much higher level than that of reovirus type 1. Using the hybrid gene constructs this higher level of protein accumulation was shown to co-segregate with the ability to stimulate reporter gene expression., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-74-6-1055