1887

Abstract

A 41K early nuclear antigen (p41), expressed in human herpesvirus type 6 (HHV-6)-infected T cells, was cloned by screening a cDNA expression library with the anti-p41 monoclonal antibody (MAb) C5. When expressed in mammalian cells, the cloned p41 protein comigrated with the authentic p41 protein from HHV-6-infected cells and localized to the nucleus. HHV-6 p41 shares 44% sequence identity with the human cytomegalovirus (HCMV) DNA-binding protein, ICP36 (UL44 gene product); p41 binds to ssDNA with the same apparent affinity as ICP36. Since ICP36 has recently been shown to be an HCMV DNA polymerase-associated stimulatory factor, a similar function is suggested for p41.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-6-1003
1993-06-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/6/JV0740061003.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-6-1003&mimeType=html&fmt=ahah

References

  1. Ablashi D. V., Josephs S. F., Buchbinder A., Hellman K., Nakamura S., Llana T., Lusso P., Kaplan M., Dahlberg J., Memon S., Imam F., Ablashi K. L., Markham P. D., Kramar-sky B., Krueger G. R. F., Biberfeld P., Wong-Staal F., Salahuddin S. Z., Gallo R. 1988a; Human B-lymphotropic virus (human herpesvirus-6). Journal of Virological Methods 21:29–48
    [Google Scholar]
  2. Ablashi D. V., Lusso P., Hung C.-L., Salahuddin S. Z., Josephs S. F., Llana T., Kramarsky B., Biberfeld P., Markham P. D., Gallo R. C. 1988b; Utilization of human hematopoietic cell lines for the propagation and characterization of HBLV (human herpesvirus 6). International Journal of Cancer 42:787–791
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1987 Current Protocols in Molecular Biology New York: John Wiley & Sons;
    [Google Scholar]
  4. Balachandran N., Amelse R. E., Zhou W. W., Chang C. K. 1989; Identification of proteins specific for human herpesvirus 6-infected human T cells. Journal of Virology 63:2835–2840
    [Google Scholar]
  5. Chang C. K., Balachandran N. 1991; Identification, characterization and sequence analysis of a cDNA encoding a phosphoprotein of human herpesvirus 6. Journal of Virology 65:2884–2894 correction 65 7085
    [Google Scholar]
  6. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., III Hutchison C. A., Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1990; Analysis of the protein coding content of the sequence of human cytomegalovirus strain AD 169. Current Topics in Microbiology and Immunology 154:125–169
    [Google Scholar]
  7. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  8. Dyson N., Bernards R., Friend S. H., Gooding L. R., Hassel J. A., Major E. O., Pipas J. M., Vandyke T., Harlow E. 1990; Large T antigens of many polyomaviruses are able to form complexes with the retinoblastoma protein. Journal of Virology 64:1353–1356
    [Google Scholar]
  9. Ensoli B., Lusso P., Schachter F., Josephs S. F., Rappaport J., Negro J., Gallo R. C., Wong-Staal F. 1989; Human herpesvirus-6 increases HIV-1 expression in co-infected T cells via nuclear factors binding to the HIV-1 enhancer. EMBO Journal 8:3019–3027
    [Google Scholar]
  10. Ertl P. F., Powell K. L. 1992; Physical and functional interaction of the human cytomegalovirus DNA polymerase and its accessory protein (ICP36) expressed in insect cells. Journal of Virology 66:4126–4133
    [Google Scholar]
  11. Gibson W., Murphy T. L., Roby C. 1981; Cytomegalovirus-infected cells contain a DNA-binding protein. Virology 111:251–262
    [Google Scholar]
  12. Gottlieb J., Marcy A. I., Coen D. M., Challberg M. D. 1990; The herpes simplex virus type 1 UL42 gene product: a subunit of DNA polymerase that functions to increase processivity. Journal of Virology 64:5976–5987
    [Google Scholar]
  13. Horvat R. T., Wood C., Balachandran N. 1989; Transactivation of human immunodeficiency virus promoter by human herpesvirus 6. Journal of Virology 63:970–973
    [Google Scholar]
  14. Iyengar S., Levine P. H., Ablashi D., Neequaye J., Pearson G. R. 1991; Sero-epidemiological investigations on human herpesvirus 6 (HHV-6) infections using a newly developed early antigen assay. International Journal of Cancer 49:551–557
    [Google Scholar]
  15. Josephs S. F., Ablashi D. V., Salahuddin S. Z., Jagodzinski L. L., Wong-Staal F., Gallo R. C. 1991; Identification of the human herpesvirus 6 glycoprotein H and putative large tegument protein genes. Journal of Virology 65:5597–5604
    [Google Scholar]
  16. Kiehl A., Dorsky D. I. 1991; Cooperation of EBV DNA polymerase and EA-D(BMRFl) in vitro and colocalization in nuclei of infected cells. Virology 184:330–340
    [Google Scholar]
  17. Kozak M. 1991; Structural features in eukaryotic mRNAs that modulate the initiation of translation. Journal of Biological Chemistry 266:19867–19870
    [Google Scholar]
  18. Lawrence G. L., Chee M., Craxton M. A., Gompels U. A., Honess R. W., Barrell B. G. 1990; Human herpesvirus 6 is closely related to human cytomegalovirus. Journal of Virology 64:287–299
    [Google Scholar]
  19. Levy J. A., Ferro F., Greenspan D., Lennette E. T. 1990; Frequent isolation of HHV-6 from saliva and high seroprevalence of the virus in the population. Lancet 335:1047–1050
    [Google Scholar]
  20. Lusso P., Ensoli B., Markham P. D., Ablashi D. V., Salahuddin S. Z., Tschachler E., Wong-Staal F., Gallo R. C. 1989; Productive dual infection of human CD4+ T lymphocytes by HIV-1 and HHV-6. Nature, London 337:370–373
    [Google Scholar]
  21. Lusso P., DeMaria A., Malnati M., Lori F., De Rocco S. E., Baseler M., Gallo R. C. 1991; Induction of CD4 and susceptibility to HIV-1 infection in human CD8+ T lymphocytes by human herpesvirus 6. Nature, London 349:533–535
    [Google Scholar]
  22. McGeoch D. J. 1989; The genomes of the human herpesviruses: contents, relationships, and evolution. Annual Review of Microbiology 43:235–265
    [Google Scholar]
  23. Marchetti M. E., Smith C. A., Schaffer P. A. 1988; A temperature-sensitive mutation in a herpes-simplex virus type 1 gene required for viral DNA synthesis maps to coordinates 0-609 through 0-614 in UL. Journal of Virology 62:715–721
    [Google Scholar]
  24. Martin M. E. D., Thomson B. J., Honess R. W., Craxton M. A., Gompels U. A., Liu M.-Y., Littler E., Arrand J. R., Teo I., Jones M. D. 1991; The genome of human herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases. Journal of General Virology 72:157–168
    [Google Scholar]
  25. Neipel F., Ellinger K., Fleckenstein B. 1991; The unique region of the human herpesvirus 6 genome is essentially collinear with the UL segment of human cytomegalovirus. Journal of General Virology 72:2293–2297
    [Google Scholar]
  26. Ricciardi R. P., Miller J. S., Roberts B. E. 1979; Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proceedings of the National Academy of Sciences, U.S.A. 76:4927–4931
    [Google Scholar]
  27. Salahuddin S. Z., Ablashi D. V., Markham P. D., Josephs S. F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-Staal F., Kramarsky B., Gallo R. C. 1986; Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science 234:596–601
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  29. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  30. Steeper T. A., Horwitz C. A., Ablashi D. V., Salahuddin S. Z., Saxinger C., Saltzman R., Schwartz B. 1990; The spectrum of clinical and laboratory findings resulting from human herpesvirus-6 (HHV-6) in patients with mononucleosis-like illness not resulting from Epstein-Barr virus or cytomegalovirus. American Journal of Clinical Pathology 93:776–783
    [Google Scholar]
  31. Teo I. A., Griffin B. E., Jones M. D. 1991; Characterization of the DNA polymerase gene of human herpesvirus 6. Journal of Virology 65:4670–4680
    [Google Scholar]
  32. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. 1988; Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet i:1065–1067
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-74-6-1003
Loading
/content/journal/jgv/10.1099/0022-1317-74-6-1003
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error