1887

Abstract

Clones have been constructed that contain full-length cDNA copies of cowpea mosaic virus RNA1 and RNA2, downstream of the cauliflower mosaic virus 35S promoter. The clones, when linearized downstream of the viral sequences, give rise to cowpea mosaic virus-like symptoms when inoculated onto cowpea plants. Viral RNA and virions can be detected in the inoculated plants, demonstrating that the clones are directly infectious.

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1993-05-01
2024-04-20
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References

  1. Commandeur U., Jarausch W., Li Y., Koenig R., Burger-meister W. 1991; cDNAs of beet necrotic yellow vein virus RNAs 3 and 4 are rendered biologically active in a plasmid containing the cauliflower mosaic virus 35S promoter. Virology 185:493–195
    [Google Scholar]
  2. Dessens J. T., Lomonossoff G. P. 1991; Mutational analysis of the putative catalytic triad of the cowpea mosaic virus 24K protease. Virology 184:738–746
    [Google Scholar]
  3. Eggen R., Verver J., Wellink J., De Jong A., Goldbach R., van Kammen A. 1989; Improvements of the infectivity of in vitro transcripts from cloned cowpea mosaic virus cDNA: impact of the terminal nucleotide sequences. Virology 173:447–455
    [Google Scholar]
  4. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  5. Goldbach R., van Kammen A. 1985 Structure, replication and expression of the bipartite genome of cowpea mosaic virus. In Molecular Plant Virology pp. 83–120 Edited by Davies J. W. Boca Raton: CRC Press;
    [Google Scholar]
  6. Hitchborn J. H., Hills G. J. 1965; The use of negative staining in the electron microscopic examination of plant viruses in crude extracts. Virology 27:528–540
    [Google Scholar]
  7. Holness C. L., Lomonossoff G. P., Evans D., Maule A. J. 1989; Identification of the initiation codons for translation of cowpea mosaic virus middle component RNA using site-directed mutagenesis of an infectious cDNA clone. Virology 172:311–320
    [Google Scholar]
  8. Lehrach H., Diamond D., Wozney J. M., Boedtker H. 1977; RNA molecular weight determinations by gel electrophoresis under denaturing conditions - a critical re-examination. Biochemistry 16:4743–4751
    [Google Scholar]
  9. Lomonossoff G. P., Shanks M. 1983; The nucleotide sequence of cowpea mosaic virus B RNA. EMBO Journal 2:2253–2258
    [Google Scholar]
  10. Lomonossoff G. P., Shanks M., Matthes M. D., Singh M., Gait M. J. 1982; The locations of the first AUG codons in cowpea mosaic virus RNAs. Nucleic Acids Research 10:4861–4872
    [Google Scholar]
  11. MacFarlane S. A., Gilmer D., Davies J. W. 1992; Efficient inoculation with CaMV 35S promoter-driven DNA clones of the tobravirus PEBV. Virology 187:829–831
    [Google Scholar]
  12. Maiss E., Timpe U., Brisske-Rode A., Lesemann D.-E., Casper R. 1992; Infectious in vivo transcripts of a plum pox potyvirus full-length cDNA clone containing the cauliflower mosaic virus 35S RNA promoter. Journal of General Virology 73:709–713
    [Google Scholar]
  13. Mori M., Mise K., Kobayashi K., Okuno T., Furusawa I. 1991; Infectivity of plasmids containing brome mosaic virus cDNA linked to the cauliflower mosaic virus 355 RNA promoter. Journal of General Virology 72:243–246
    [Google Scholar]
  14. Rohll J. B., Holness C. L., Lomonossoff G. P., Maule A. J. 1993 3′ terminal nucleotide sequences important for the accumulation of cowpea mosaic virus M-RNA. Virology (in press)
    [Google Scholar]
  15. van Wezenbeek P., Verver J., Harmsen J., Vos P., van Kammen A. 1983; Primary structure and gene organization of the middle component RNA of cowpea mosaic virus. EMBO Journal 2:941–946
    [Google Scholar]
  16. Verwoerd T. C., Dekker B. M. M., Hobkema A. 1989; A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17:2362
    [Google Scholar]
  17. Vos P., Jaegle M., Wellink J., Verver J., Eggen R., van Kammen A., Goldbach R. 1988; Infectious RNA transcripts derived from full-length DNA copies of the genomic RNAs of cowpea mosaic virus. Virology 165:33–11
    [Google Scholar]
  18. Weber H., Haeckel P., Pfitzner A. J. P. 1992; A cDNA clone of tomato mosaic virus is infectious in plants. Journal of Virology 66:3909–3912
    [Google Scholar]
  19. Wellink J., van Kammen A. 1989; Cell-to-cell transport of cowpea mosaic virus requires both the 58K/48K proteins and the capsid proteins. Journal of General Virology 70:2279–2286
    [Google Scholar]
  20. Yamaya J., Yoshioka M., Meshi T., Okada Y., Ohno T. 1988; Expression of tobacco mosaic virus RNA in transgenic plants. Molecular and General Genetics 211:520–525
    [Google Scholar]
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