We report the first complete nucleotide sequence of the picornavirus coxsackievirus B5 (CB5), strain 1954/UK/85, an isolate from a case of hand-foot-and-mouth disease. We have compared the sequence with those of other coxsackie B viruses, coxsackievirus A9, poliovirus and swine vesicular disease virus (SVDV). The genes encoding the three major capsid proteins are most closely related to those of SVDV but the 5′ and 3′ non-coding regions and the P3 gene are more similar to the corresponding regions in the other coxsackie B viruses than to those of SVDV. These observations are considered in the light of the antigenic and biochemical relationships between SVDV and CB5.
BrownF.,
TalbotP.,
BurrowsR.1973; Antigenic differences between isolates of swine vesicular disease virus and their relationship to coxsackie B5 virus. Nature, London 245:315–316
BrownF.,
WildT. F.,
RoweL. W.,
UnderwoodB. O.,
HarrisT. J. R.1976b; Comparison of swine vesicular disease virus and coxsackie B5 virus by serological and RNA hybridization methods. Journal of General Virology 31:231–237
ChangK. H.,
AuvinenP.,
HyypiäT.,
StanwayG.1989; The nucleotide sequence of coxsackievirus A9; implications for receptor binding and enterovirus classification. Journal of General Virology 70:3269–3280
FilmanD. J.,
SyedR.,
ChowM.,
MacAdamA. J.,
MinorP. D.,
HogleJ. M.1989; Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8:1567–1579
HarrisT. J. R.,
DoelT. R.,
BrownF.1977; Molecular aspects of the antigenic variation of swine vesicular disease and coxsackie B5 viruses. Journal of General Virology 35:299–315
HughesP. J.,
EvansD. M. A.,
MinorP. D.,
SchildG. C.,
AlmondJ. W.,
StanwayG.1986; The nucleotide sequence of a type 3 poliovirus isolated during a recent outbreak of poliomyelitis in Finland. Journal of General Virology 67:2093–2102
KewO.,
NottayB. K.1984 Evolution of the oral polio vaccine strains in humans occurs by both mutation and intermolecular recombination. In Molecular and Chemical Basis of Virus Virulence and Immunogenicity, Modern Approaches to Vaccines, vol. 2 pp. 357–362 Edited by
ChanockR. M.,
LernerR. A.
New York: Cold Spring Harbor Laboratory;
KitamuraN.,
SemlerB. L.,
RothbergP. G.,
LarsenG. R.,
AdlerC. J.,
DornerA. J.,
EminiE. A.,
HanecakR.,
LeeJ. J.,
van der WerfS.,
AndersonC. W.,
WimmerE.1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature, London 291:547–553
LaiS. S.,
McKercherP. D.,
MooreD. M.,
GillespieJ. H.1980; Response of pigs to recent isolates of coxsackievirus B5. Comparative Immunology, Microbiology and Infectious Diseases 2:459–168
LipskayaG. Y.,
MuzychenkoA. R.,
KutitovaO. K.,
MaslovaS. V.,
EquestreM.,
DrozdovS. G.,
Perez-BercoffR.,
AgolV. I.1991; Frequent isolation of intertypic poliovirus recombinants with serotype-2 specificity from vaccine-associated polio cases. Journal of Medical Virology 35:290–296
MonluxW. S.,
McKercherP. D.,
GravesJ. H.1975; Brain and spinal cord lesions in pigs inoculated with swine vesicular disease (UKG strain) virus and coxsackievirus B5. American Journal of Veterinary Research 36:1745–1749
PallanschM. A.,
FreemanC. Y.1987 Genetic variation and phylogenetic relationships among coxsackie B virus isolates and identification of conserved sequences as targets for pan-enterovirus oligonucleotide probes. Abstract No. R16.25, 7th International Congress of Virology, Edmonton, Canada, August 1987
PilipenkoE. V.,
BlinovV. M.,
RomanovaL. L.,
SinyakovA. N.,
MaslovaS. V.,
AgolV. I.1989; Conserved structural domains in the 5′ untranslated region of picomaviral genomes: an analysis of the segment controlling translation and neurovirulence. Virology 168:201–209
RacanielloV. R.,
BaltimoreD.1981; Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proceedings of the National Academy of Sciences, U.S.A. 78:4887–4891
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
SkinnerM. A.,
RacanielloV. R.,
DunnG.,
CooperJ.,
MinorP. D.,
AlmondJ. W.1989; New model for the secondary structure of the 5′ non-coding RNA of poliovirus is supported by biochemical and genetic data that show that RNA secondary structure is important in neuro virulence. Journal of Molecular Biology 207:379–392
StadenR.1987 Computer handling of DNA sequencing projects. In Nucleic Acid and Protein Sequence Analysis: A Practical Approach pp. 173–217 Edited by
BishopM. J.,
RawlingsC. J.
Oxford: IRL Press;
WatsonC. J.,
JacksonJ. F.1985 An alternative procedure for the synthesis of double-stranded cDNA for cloning in phage and plasmid vectors. In DNA Cloning: A Practical Approach vol. 1 pp. 79–88 Edited by
GloverD. M.
Oxford: IRL Press;
WernerG.,
RosenwirthB.,
BaurerE.,
SeifertJ.-M.,
WernerF.-J.,
BesemerJ.1986; Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses. Journal of Virology 57:1084–1093