1887

Abstract

Although rabies outbreaks in most parts of the world tend to be host species-specific the rabies currently enzootic in the Canadian province of Ontario is hosted by two wildlife species, the red fox and the striped skunk. Previous studies employing monoclonal antibody panels failed to identify any host-specific differences in Ontario rabies virus street isolates, but certain observations suggested the existence of more than one viral strain in terrestrial mammals of this region. The extent of variation of the rabies virus circulating within this region has been re-examined using molecular biology techniques. The N gene of several independent isolates was amplified using PCR and the resulting products were compared by restriction enzyme analysis and, in some cases, by DNA sequencing. This analysis confirmed that there was indeed no host-specific variation in the portion of the viral genome under study but there were, however, very clear and consistent differences in the virus from distinct geographical regions.

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1993-05-01
2022-08-15
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References

  1. Benmansour A., Brahimi M., Tuffereau C., Coulon P., Lafay F., Flamand A. 1992; Rapid sequence evolution of street rabies glycoprotein is related to the highly heterogeneous nature of the viral population. Virology 187:33–45
    [Google Scholar]
  2. Bourhy Fl., Kissi B., Lafon M., Sacramento D., Tordo N. 1992; Antigenic and molecular characterization of bat rabies virus in Europe. Journal of Clinical Microbiology 30:2419–2426
    [Google Scholar]
  3. Charlton K. M. 1988 The pathogenesis of rabies. In Rabies pp. 101–150 Edited by Campbell J. B., Charlton K. M. Boston: Kluwer Academic Publishers;
    [Google Scholar]
  4. Conzelmann K.-K., Cox J. H., Schneider L. G., Thiel H.-J. 1990; Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19. Virology 175:485–499
    [Google Scholar]
  5. Crysler J. G., Lee P., Reinders M., Prevec L. 1990; The sequence of the nucleocapsid protein (N) gene of Piry virus: possible domains in the N protein of vesiculoviruses. Journal of General Virology 71:2191–2194
    [Google Scholar]
  6. Erlich H. A., Gelfand D. H., Saiki R. K. 1988; Specific DNA amplification. Nature, London 331:461–462
    [Google Scholar]
  7. Erlich H. A., Gelfand D., Sninsky J. J. 1991; Recent advances in the polymerase chain reaction. Science 252:1643–1651
    [Google Scholar]
  8. Ertl H. C. J., Dietzschold B., Gore M., Otvos L. Jr, Larson J. K., Wunner W. H., Koprowski H. 1989; Induction of rabies virus-specific T-helper cells by synthetic peptides that carry dominant T-helper cell epitopes of the viral ribonucleoprotein. Journal of Virology 63:2885–2892
    [Google Scholar]
  9. Gould A. R. 1987; The complete nucleotide sequence of bluetongue virus serotype 1 RNA3 and a comparison with other geographic serotypes from Australia, South Africa and the United States of America, and with other orbivirus isolates. Virus Research 1:169–183
    [Google Scholar]
  10. Gould A. R., Pritchard L. I. 1990; Relationships amongst bluetongue viruses revealed by comparisons of capsid and outer coat protein nucleotide sequences. Virus Research 17:31–52
    [Google Scholar]
  11. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  12. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
    [Google Scholar]
  13. Mannen K., Hiramatsu K., Mifune K., Sakamoto S. 1991; Conserved nucleotide sequence of rabies virus cDNA encoding the nucleoprotein. Virus Genes 5:69–73
    [Google Scholar]
  14. Rosatte R. C. 1988; Rabies in Canada: history, epidemiology and control. Canadian Veterinary Journal 29:362–365
    [Google Scholar]
  15. Rupprecht C. E., Dietzschold B., Wunner W. H., Koprowski H. 1991 Antigenic relationships of lyssaviruses. In The Natural History of Rabies pp. 69–100 Edited by Baer G. M. Boca Raton: CRC Press;
    [Google Scholar]
  16. Sacramento D., Bourhy H., Tordo N. 1991; PCR technique as an alternative method for diagnosis and molecular epidemiology of rabies virus. Molecular and Cellular Probes 5:229–240
    [Google Scholar]
  17. Sacramento D., Badrane H., Bourhy H., Tordo N. 1992; Molecular epidemiology of rabies virus in France: comparison with vaccine strains. Journal of General Virology 73:1149–1158
    [Google Scholar]
  18. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Smith J. S. 1989; Rabies virus epitopic variation: use in ecologie studies. Advances in Virus Research 36:215–253
    [Google Scholar]
  20. Smith J. S., Reid-Sanden F. L., Roumillat L. F., Trimarchi C., Clark K., Baer G. M., Winkler W. G. 1986; Demonstration of antigenic variation among rabies virus isolates by using monoclonal antibodies to nucleocapsid proteins. Journal of Clinical Microbiology 24:573–580
    [Google Scholar]
  21. Steinhauer D. A., Holland J. J. 1987; Rapid evolution of RNA viruses. Annual Review of Microbiology 41:409–433
    [Google Scholar]
  22. Tabel H., Corner A. H., Webster W. A., Casey G. A. 1974; History and epizootiology of rabies in Canada. Canadian Veterinary Journal 15:271–281
    [Google Scholar]
  23. Tinline R. R. 1988 Persistence of rabies in wildlife. In Rabies pp. 301–322 Edited by Campbell J. B., Charlton K. M. Boston: Kluwer Academic Publishers;
    [Google Scholar]
  24. Tordo N., Poch O., Ermine A., Keith G. 1986; Primary structure of leader RNA and nucleoprotein genes of the rabies genome: segmented homology with VSV. Nucleic Acids Research 14:2671–2683
    [Google Scholar]
  25. Uryu N., Maeda M., Ota M., Tsuji K., Inoko H. 1990; A simple and rapid method for HLA-DRB and -DQB typing by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 35:20–31
    [Google Scholar]
  26. Wandeler A. I. 1987 Virus infections of non-domestic carnivores: rabies virus. In Virus Infections of Carnivores pp. 449–461 Edited by Appel M. J. Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  27. Webster W. A., Casey G. A. 1988 Diagnosis of rabies infection. In Rabies pp. 201–222 Edited by Campbell J. B., Charlton K. M. Boston: Kluwer Academic Publishers;
    [Google Scholar]
  28. Webster W. A., Casey G. A., Charlton K. M., Wiktor T. J. 1985; Antigenic variants of rabies virus in isolates from eastern, central and northern Canada. Canadian Journal of Comparative Medicine 49:186–188
    [Google Scholar]
  29. Webster W. A., Casey G. A., Charlton K. M. 1986; Major antigenic groups of rabies virus in Canada determined by anti-nucleocapsid monoclonal antibodies. Comparative Immunology, Microbiology and Infectious Diseases 9:59–69
    [Google Scholar]
  30. Wunner W. H. 1991 The chemical composition and molecular structure of rabies viruses. In The Natural History of Rabies pp. 31–67 Edited by Baer G. M. Boca Raton: CRC Press;
    [Google Scholar]
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