1887

Abstract

Human sera were tested for antibodies against the Epstein-Barr virus (EBV) latent infection terminal proteins (TPs). Anti-TP IgG and IgA antibodies were detected by an indirect immunofluorescence assay of insect cells expressing a recombinant TP1. Out of 301 human sera of patients with EBV-related and EBV-unrelated disorders, only sera from patients with nasopharyngeal carcinoma (NPC) (32/83; 38%) showed anti-TP antibodies. Studies on serial sera from German and Hong Kong NPC patients revealed a decline of anti-TP antibodies during tumour therapy, and none of these antibodies were identified in patients with early tumour stages or in remission. Comparative studies of TP1-specific polyclonal rabbit antisera and human TP-positive sera showed clear differences in the TP epitopes recognized by each. Human antisera contained antibodies only to native epitopes in exons 2 to 7 of TP1 whereas rabbit antisera reacted only with epitopes located in the first exon and, additionally, exhibited EBV strain specificities.

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1993-05-01
2024-12-12
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References

  1. Brooks L., Yao Q. Y., Rickinson A. B., Young L. S. 1992; Epstein-Barr virus latent gene transcription in nasopharyngeal carcinoma cells: coexpression of EBNA1, LMP1, and LMP2 transcripts. Journal of Virology 66:2689–2697
    [Google Scholar]
  2. Busson P., McCoy R., Sadler R., Gilligan K., Tursz T., Raab-Traub N. 1992; Consistent transcription of the Epstein-Barr virus LMP2 gene in nasopharyngeal carcinoma. Journal of Virology 66:3257–3262
    [Google Scholar]
  3. Chen J. Y., Hwang L. Y., Beasley R. P., Chien C. S., Yang C. S. 1985; Antibody response to Epstein-Barr virus-specific DNAse in 15 patients with nasopharyngeal carcinoma in Taiwan: a retrospective study. Journal of Medical Virology 244:245–247
    [Google Scholar]
  4. Crawford D. H., Epstein M. A., Bornkamm G. W., Achong B. G., Finerty S., Thompson J. 1979; Biological and biochemical observations on isolates of EB virus from the malignant epithelial cells of two nasopharyngeal carcinomas. International Journal of Cancer 24:294–302
    [Google Scholar]
  5. De The G. 1982 Epidemiology of Epstein-Barr virus and associated diseases in man. In The Herpesviruses vol. 1 pp. 25–103 Edited by Roizman B. New York: Plenum Press;
    [Google Scholar]
  6. Fahraeus R., Fu J. L., Ernberg I., Finke J., Rowe M., Klein G., Nilsson E., Yadav M., Busson P., Tursz T., Kallin B. 1988; Expression of Epstein-Barr virus encoded proteins in nasopharyngeal carcinoma. International Journal of Cancer 42:329–338
    [Google Scholar]
  7. Foong Y. T., Cheng H. M., Sam C. K., Dillner J., Hinderer W., Prasad U. 1990; Serum and salivary IgA antibodies against a defined epitope of the Epstein-Barr virus nuclear antigen (EBNA) are elevated in nasopharyngeal carcinoma. International Journal of Cancer 45:1061–1064
    [Google Scholar]
  8. Frech B., Zimber-Strobl U., Suentzenich K.-O., Pavlish O., Lenoir G. M., Bornkamm G. W., Mueller-Lantzsch N. 1990; Identification of Epstein-Barr virus terminal protein 1 (TP1) in extracts of four lymphoid cell lines, expression in insect cells and detection of antibodies in human sera. Journal of Virology 64:2759–2767
    [Google Scholar]
  9. Hadar T., Rakina M., Kala E. 1986; Significance of specific Epstein-Barr virus IgA and elevated IgG antibodies to viral capsid antigens in nasopharyngeal carcinoma patients. Journal of Medical Virology 20:329–339
    [Google Scholar]
  10. Henle G., Henle W. 1976; Epstein-Barr virus-specific IgA serum antibodies as an outstanding feature of nasopharyngeal carcinoma. International Journal of Cancer 17:1–7
    [Google Scholar]
  11. Henle W., Ho J. H. C., Henle G., Kwan H. C. 1973; Antibodies to Epstein-Barr virus related antigens in nasopharyngeal carcinoma. Comparison of active cases and long term survivors. Journal of the National Cancer Institute 51:361–369
    [Google Scholar]
  12. Henle W., Ho J. H. C., Henle G., Chou J. C. W., Kwan H. C. 1977; Nasopharyngeal carcinoma: significance of changes in Epstein-Barr virus related antibody patterns following therapy. International Journal of Cancer 20:663–672
    [Google Scholar]
  13. Herbst H., Dallenbach F., Hummel M., Niedobitek G., Pileri S., Mueller-Lantzsch N., Stein H. 1991; Epstein-Barr virus latent membrane protein expression in Hodgkin and Reed-Sternberg cells. Proceedings of the National Academy of Sciences, U.S.A. 88:4766–4770
    [Google Scholar]
  14. Hinuma Y., Kann M., Yamaguchi I., Wudarski J., Blakestee J. R., Grace J. T. 1967; Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. Journal of Virology 1:1045–1051
    [Google Scholar]
  15. Kieff E., Liebowitz D. 1990 Epstein-Barr virus and its replication. In Virology, 2nd edn. pp. 1889–1920 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  16. Klein G., Giovanella B. C., Lindahl T., Fialkow P. J., Singh S., Stehlin J. S. 1974; Direct evidence for the presence of Epstein-Barr virus DNA and nuclear antigen in malignant epithelial cells from patients with poorly differentiated carcinoma of nasopharynx. Proceedings of the National Academy of Sciences, U.S.A. 71:4737–4741
    [Google Scholar]
  17. Laux G., Perricaudet M., Farrell P. J. 1988; A spliced Epstein-Barr virus gene expressed in immortalized lymphocytes is created by circularization of the linear viral genome. EMBO Journal 1:769–774
    [Google Scholar]
  18. Lenoir G. M., Vuillaume M., Bonnardel C. 1985 The use of lymphomatous and lymphoblastoid cell lines in the study of Burkitt’s lymphoma. In Burkitt’s Lymphoma: A Human Cancer Model pp. 309–318 Edited by Lenoir G. M., O’Conor G., Olweny C. L. Lyon: IARC Scientific Publications;
    [Google Scholar]
  19. Longnecker R., Kieff E. 1990; A second Epstein-Barr virus membrane protein (LMP2) is expressed in latent infection and colocalizes with LMP1. Journal of Virology 64:2319–2326
    [Google Scholar]
  20. Longnecker R., Druker B., Roberts T. M., Kieff E. 1991; An Epstein-Barr virus protein associated with cell growth transformation interacts with a tyrosine kinase. Journal of Virology 65:3681–3692
    [Google Scholar]
  21. Mann K. P., Stanton D., Thorley-Lawson D. A. 1985; Epstein-Barr virus-encoded protein found in plasma membranes of transformed cells. Journal of Virology 55:710–720
    [Google Scholar]
  22. Miller G., Lipman M. 1973; Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proceedings of the National Academy of Sciences, U.S.A. 70:190–194
    [Google Scholar]
  23. Old L. J., Clifford P., Boyse E. A., De Harven E., Geering G., Oettgen H. F., Williamson B. 1966; Precipitating antibody in human serum to an antigen present in cultured Burkitt’s lymphoma cells. Proceedings of the National Academy of Sciences, U.S.A. 56:1699–1704
    [Google Scholar]
  24. Pallesen G., Hamilton-Dutoit S. J., Rowe M., Young L. S. 1991; Expression of Epstein-Barr virus latent gene products in tumour cells of Hodgkin’s disease. Lancet 337:320–322
    [Google Scholar]
  25. Pulvertaft R. J. V. 1965; A study of malignant tumors in Nigeria by short term tissue culture. Journal of Clinical Pathology 18:261–273
    [Google Scholar]
  26. Raab-Traub N., Hood R., Yang C. S., Henry B., Pagano J. S. 1983; Epstein-Barr virus transcription in nasopharyngeal carcinoma. Journal of Virology 48:580–590
    [Google Scholar]
  27. Rowe D. T., Hall L., Joab I., Laux G. 1990; Identification of the Epstein-Barr virus terminal protein gene products in latently infected lymphocytes. Journal of Virology 64:2866–2875
    [Google Scholar]
  28. Rowe M., Finke J., Szigeti R., Klein G. 1988; Characterization of the serological response in man to the latent membrane protein and the six nuclear antigens encoded by Epstein-Barr virus. Journal of General Virology 69:1217–1228
    [Google Scholar]
  29. Sample J., Liebowitz D., Kieff E. 1989; Two related Epstein-Barr virus membrane proteins are encoded by separate genes. Journal of Virology 63:933–937
    [Google Scholar]
  30. Seigneurin J. M., La voue M. F., Genoulaz O., Bornkamm G. W., Lenoir G. M. 1987; Antibody response against the Epstein-Barr virus-encoded nuclear antigen 2 (EBNA2) in different groups of individuals. International Journal of Cancer 40:349–353
    [Google Scholar]
  31. Simanis V., Lane D. P. 1985; An immunoaffinity procedure for SV40 large T antigen. Virology 144:88–100
    [Google Scholar]
  32. Sixbey J. W., Yao Q.-Y. 1992; Immunoglobulin A-induced shift of Epstein-Barr virus tissue tropism. Science 255:1578–1580
    [Google Scholar]
  33. Smith P. R., Griffin B. E. 1991; Differential expression of Epstein-Barr viral transcripts for two proteins (TP1 and LMP) in lymphocyte and epithelial cells. Nucleic Acids Research 19:2435–2440
    [Google Scholar]
  34. Spindler K. R., Rosser D. S. E., Berk A. J. 1984; Analysis of adenovirus transforming proteins from early regions 1A and IB with antisera to inducible fusion antigens produced in Escherichia coli. Journal of Virology 49:132–141
    [Google Scholar]
  35. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect culture procedures. Texas Agricultural Experiment Station Bulletin no. 1555
    [Google Scholar]
  36. Tan R. S., Cheng Y. C., Naegele R. F., Henry W., Glaser R., Champion J. 1982; Antibody responses to Epstein-Barr virus specific DNAase in relation to the prognosis of juvenile patients with nasopharyngeal carcinoma. International Journal of Cancer 30:561–565
    [Google Scholar]
  37. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
    [Google Scholar]
  38. Waterhouse J., Muir C. S., Shanmugaratnam K., Powell J. 1982 Cancer Incidence in Five Continents vol. IV Lyon: IARC Scientific Publication; no. 42
    [Google Scholar]
  39. Wolf H., zur Hausen H., Becker V. 1973; EB viral genomes in epithelial nasopharyngeal carcinoma cells. Nature, London 244:245–247
    [Google Scholar]
  40. Young L. S., Dawson C. W., Clark D., Rupani H., Busson P., Tursz T., Johnson A., Rickinson A. B. 1988; Epstein-Barr virus gene expression in nasopharyngeal carcinoma. Journal of General Virology 69:1051–1065
    [Google Scholar]
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