1887

Abstract

The complete nucleotide sequence (3514 nucleotides) of RNA segment 2 of rice stripe virus (RSV), the prototype member of tenuivirus group, was determined. In the virus-sense RNA an open reading frame (ORF) is present which encodes a 199 amino acid protein of 22762. Another long ORF encoding an 834 amino acid protein with 94047 (94K) exists in the virus-complementary RNA. Between these two ORFs, there is a long non-coding intergenic region of 299 nucleotides. The sequence suggests that RNA 2 has an ambisense coding strategy as found for RSV RNAs 3 and 4. The putative 94K protein carries stretches with an amino acid sequence showing weak similarity to parts of the membrane glycoproteins of Punta Toro and Uukuniemi phleboviruses of the family Bunyaviridae, suggesting a possible distinct evolutionary relationship between the animal phleboviruses and the plant tenuiviruses.

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1993-04-01
2024-04-20
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References

  1. Barbier P., Takahashi M., Nakamura I., Toriyama S., Ishihama A. 1992; Solubilization and promoter analysis of RNA polymerase from rice stripe virus. Journal of General Virology 66:1671–1674
    [Google Scholar]
  2. Elliott R. M. 1990; Molecular biology of the Bunyaviridae. Journal of General Virology 71:501–522
    [Google Scholar]
  3. Francki R. IB., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and nomenclature of viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology supplementum 2398–399 Wien & New York: Springer-Verlag;
    [Google Scholar]
  4. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25263–269
    [Google Scholar]
  5. Hanahan D. 1985; Techniques for transformation of Esherichia coli. In DNA Cloning: A Practical Approach vol 1 pp 109–135 Edited by Glover D. M. Oxford & Washington, D.C.: IRL Press;
    [Google Scholar]
  6. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  7. Huiet L., Klaassen V., Tsai J. H., Falk B. W. 1991; Nucleotide sequence and RNA hybridization analyses reveal an ambisense coding strategy for maize stripe virus RNA3. Virology 182:47–53
    [Google Scholar]
  8. Huiet L., Tsai J. H., Falk B. W. 1992; Complete sequence of maize stripe virus RNA4 and mapping of its subgenomic RNAs. Journal of General Virology 73:1603–1607 corrigendum 3049
    [Google Scholar]
  9. Ihara T., Smith J., Dalrymple J. M., Bishop D. HL. 1985; Complete sequences of the glycoproteins and M RNA of Punta Toro phlebovirus compared to those of Rift Valley fever virus. Virology 144:246–259
    [Google Scholar]
  10. Kakutani T., Hayano Y., Hayashi T., Minobe Y. 1990; Ambisense segment 4 of rice stripe virus: possible evolutionary relationship with phleboviruses and uukuviruses (Bunyaviridae). Journal of General Virology 71:1427–1432
    [Google Scholar]
  11. Kakutani T., Hayano Y., Hayashi T., Minobe Y. 1991; Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments. Journal of General Virology 72:465–468
    [Google Scholar]
  12. Law M. D., Speck J., Moyer J. W. 1992; The M RNA of impatiens necrotic spot tospovirus (Bunyaviridae) has an ambisense genomic organization. Virology 188:732–741
    [Google Scholar]
  13. Ronnholm R., Petterson R. F. 1987; Complete nucleotide sequence of the M RNA segment of Uukuniemi virus encoding the membrane glycoproteins G1 and G2. Virology 160:191–202
    [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  15. Takahashi M., Toriyama S., Kikuchi Y., Hayakawa T. 1990; Complementarity between the 5ʹ- and 3ʹ-terminal sequences of rice stripe virus RNAs. Journal of General Virology 71:2817–2821
    [Google Scholar]
  16. Toriyama S. 1982; Characterization of rice stripe virus: a heavy component carrying infectivity. Journal of General Virology 61:187–195
    [Google Scholar]
  17. Toriyama S. 1983; Rice stripe virus. CMI/AAB Descriptions of Plant Viruses no. 269
    [Google Scholar]
  18. Toriyama S. 1986a; An RNA-dependent RNA polymerase associated with the filamentous nucleoproteins of rice stripe virus. Journal of General Virology 61:1247–1255
    [Google Scholar]
  19. Toriyama S. 1986b; Rice stripe virus: prototype of a new group of viruses that replicate in plants and insects. Microbiological Sciences 3:347–351
    [Google Scholar]
  20. Toriyama S. 1987; Ribonucleic acid polymerase activity in filamentous nucleoproteins of rice grassy stunt virus. Journal of General Virology 68:925–929
    [Google Scholar]
  21. Toriyama S., Watanabe Y. 1989; Characterization of single- and double-stranded RNAs in particles of rice stripe virus. Journal of General Virology 70:505–511
    [Google Scholar]
  22. von Bonsdorff C. H., Saikku P., Oker-Blom N. 1969; The inner structure of Uukuniemi virus and two Bunyamwera supergroup arborviruses. Virology 39:342–344
    [Google Scholar]
  23. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  24. Zhu Y., Hayakawa T., Toriyama S., Takahashi M. 1991; Complete nucleotide sequence of RNA 3 of rice stripe virus: an ambisense coding strategy. Journal of General Virology 72:763–767
    [Google Scholar]
  25. Zhu Y., Hayakawa T., Toriyama S. 1992; Complete nucleotide sequence of RNA 4 of rice stripe virus isolate T, and comparison with another isolate and with maize stripe virus. Journal of General Virology 73:1309–1312
    [Google Scholar]
  26. Zimmern D., Kaesberg P. 1978; 3ʹ-Terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 75:4257–4261
    [Google Scholar]
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