%0 Journal Article %A Wolstenholme, Adrian J. %A Coates, J. Paul %A Douglass, Wendy %T Expression of hepatitis A virus poly(U) polymerase in the periplasmic space of Escherichia coli %D 1993 %J Journal of General Virology, %V 74 %N 3 %P 531-534 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-74-3-531 %I Microbiology Society, %X A cDNA containing almost all of the hepatitis A virus (HAV) P3 sequences was expressed as a fusion protein with Protein A. A novel poly(U) polymerase activity was detected in the periplasmic space of Escherichia coli cells transformed with this plasmid, and this activity showed many of the expected properties of a picornavirus 3Dpol. A number of HAV-specific polypeptides were detected in these cells, and it is unclear which of these was responsible for the polymerase activity. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-74-3-531