Analysis of Epstein—Barr virus gene transcription in lymphoma induced by the virus in the cottontop tamarin by construction of a cDNA library with RNA extracted from a tumour biopsy Free

Abstract

Inoculation of the cottontop tamarin with Epstein—Barr virus (EBV) gives rise to the development of mono- and/or oligoclonal large cell malignant lymphoma. A cDNA library was generated with the RNA extracted from an EBV-induced tamarin lymphoma biopsy in order to study the transcripts expressed in the tumour tissue. Fifteen EBV-specific cDNA clones were localized in the corresponding viral genomic fragments. Among them, two correspond to the EBNA-2 gene, and two others to the latent membrane protein gene. The majority of the cDNA clones were localized in the HI A fragment which has not been associated with latent expression. Furthermore, cDNAs were also found from the HI D and I fragments. Sequence analysis of the cDNAs localized in HI A showed that they correspond to a rightward transcript in the BALF-3 region, with the one clone that was sequenced containing four exons and three introns. The above results were confirmed by testing three different biopsies with the rapid amplification of cDNA ends-PCR method.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-3-509
1993-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/3/JV0740030509.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-3-509&mimeType=html&fmt=ahah

References

  1. Arrand J. R., Rymo L. 1982; Characterization of the major Epstein-Barr virus-specific RNA in Burkitt lymphoma-derived cells. Journal of Virology 41:376–389
    [Google Scholar]
  2. Baer R., Bankier A., Biggin M., Deininger P., Farrell P., Gibson T., Hatful G., Hudson G., Satchwell S., Seguin C., Tuffnell P., Barrell B. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus. Nature, London 310:207–211
    [Google Scholar]
  3. Bankier A. T., Deininger P. L., Satchwell S. C., Baer R., Farrell P. J., Barrell B. G. 1983; DNA sequence analysis of the EcoRl-Dhet fragment of B95-8 Epstein-Barr virus containing the terminal repeat sequences. Molecular Biology and Medicine 1:425–445
    [Google Scholar]
  4. Cleary M. L., Epstein M. A., Finerty S., Dorfman R. F., Bornkamm G. W., Kirkwood J. K., Morgan A. J., Sklar J. 1985; Individual tumors of multifocal EB virus-induced malignant lymphomas in tamarins arise from different B-cell clones. Science 228:722–724
    [Google Scholar]
  5. Cohen J. I., Wang F., Mannick J., Kieff E. 1989; Epstein-Barr virus nuclear protein 2 is a key determinant of lymphocyte transformation. Proceedings of the National Academy of Sciences, U.S.A. 86:9558–9562
    [Google Scholar]
  6. Dambaugh T., Hennessy K., Fennewald S., Kieff E. 1986; The EBV genome and its expression in latent infection. In The Epstein Barr Virus: Recent Advances pp. 13–45 Edited by Epstein M. A., Achong B. G. London: Heinemann Medical Books;
    [Google Scholar]
  7. Decaussin G., Finerty S., Zhang C. X., Morgan A., Ooka T. 1989; Epstein-Barr virus mRNA expression in biopsies of lymphoma induced by the virus in the cottontop tamarin. In Epstein-Barr Virus and Human Disease pp. 149–153 Edited by Ablashi D. V. Clifton, New Jersey: Humana Press;
    [Google Scholar]
  8. Epstein M. A., Achong B. 1979 The Epstein-Barr Virus. London: Springer-Verlag;
    [Google Scholar]
  9. Epstein M. A., Achong B. G. 1986 The Epstein-Barr Virus. Recent Advances. London: Heinemann Medical Books;
    [Google Scholar]
  10. Epstein M. A., Achong B. G., Barr Y. 1965; Morphological and biological studies on a virus in cultured lymphoblasts from Burkitt’s lymphoma. Journal of Experimental Medicine 121:761–770
    [Google Scholar]
  11. Gilligan K., Sato EL, Rajadurai P., Busson P., Young L., Rickinson A., Tursz T., Raab-Traub N. 1990; Novel transcription from the Epstein-Barr virus terminal BcoRI fragment, DIJhet, in a nasopharyngeal carcinoma. Journal of Virology 64:4948–4956
    [Google Scholar]
  12. Hammerschmidt W., Sugden B. 1989; Genetic analysis of immortalizing functions of Epstein-Barr virus in human B lymphocytes. Nature, London 340:393–397
    [Google Scholar]
  13. Henlé W., Henlé G., Ho H.-O., Burtin P., Cachin Y., Clifford P., De Schryver A., De-Thé G., Diehl V., Klein G. 1970; Antibodies to Epstein-Barr virus in nasopharyngeal carcinoma, other head and neck neoplasms, and control groups. Journal of the National Cancer Institute 44:225–231
    [Google Scholar]
  14. Hitt M. M., Allday M. J., Hara T., Karran L., Jones M. D., Busson P., Tursz T., Ernberg I., Griffin B. E. 1989; EBV gene expression in an NPC-related tumour. EMBO Journal 8:2639–2651
    [Google Scholar]
  15. Raab-Traub N., Hood R., Yang C. S., Henry B., Pagano J. S. 1983; Epstein-Barr virus transcription in nasopharyngeal carcinoma. Journal of Virology 48:580–590
    [Google Scholar]
  16. Rowe M., Rowe D. T., Gregory C. D., Young L. S., Farrell P. J., Rupani H., Rickinson A. B. 1987; Differences in B cell growth phenotype reflect novel patterns of Epstein-Barr virus latent gene expression in Burkitt’s lymphoma cells. EMBO Journal 6:2743–2751
    [Google Scholar]
  17. Rymo I. 1979; Identification of transcribed regions of Epstein-Barr virus DNA in Burkitt lymphoma-derived cells. Journal of Virology 32:8–18
    [Google Scholar]
  18. Skare J., Farley J., Strominger J. L., Fresen O., Cho M. S., Zur Hausen H. 1985; Transformation by Epstein-Barr virus requires DNA sequences in the region of BamHI fragments Y and H. Journal of Virology 55286–297
    [Google Scholar]
  19. Tugwood J. D., Lau W. H., O S.-K., Tsao S.-Y., Martin W. M., Shiu W., Desgranges C., Jones P. H., Arrand J. R. 1987; Epstein-Barr virus-specific transcription in normal and malignant nasopharyngeal biopsies and in lymphocytes from healthy donors and infectious mononucleosis patients. Journal of General Virology 68:1081–1091
    [Google Scholar]
  20. Wang D., Liebowitz D., Kieff E. 1985; An EBV membrane protein expressed in immortalized lymphocytes transforms established rodent cells. Cell 43:831–840
    [Google Scholar]
  21. Wang D., Liebowitz D., Wang F., Gregory C. D., Rickinson A. B., Larson R., Springer T., Kieff E. 1988; Epstein-Barr virus latent infection membrane protein alters the human B-lymphocyte phenotype: deletion of the amino terminus abolishes activity. Journal of Virology 62:4173–4184
    [Google Scholar]
  22. Wang H., Wu J. J., Tang P. S. 1989; Superfamily expands. Nature, London 337:514
    [Google Scholar]
  23. Wei M. X., Ooka T. 1989; A transforming function of the BARF 1 gene encoded by Epstein-Barr virus. EMBO Journal 8:2897–2903
    [Google Scholar]
  24. Young L. S., Dawson C. W., Clark D., Rupani H., Busson P., Tursz T., Johnson A., Rickinson A. B. 1988; Epstein-Barr virus gene expression in nasopharyngeal carcinoma. Journal of General Virology 69:1051–1065
    [Google Scholar]
  25. Young L. S., Finerty S., Brooks L., Scullion F., Rickinson A. B., Morgan A. J. 1989; Epstein-Barr virus gene expression in malignant lymphomas induced by experimental virus infection of cottontop tamarins. Journal of Virology 63:1967–1974
    [Google Scholar]
  26. Zeng Y. 1985; Seroepidemiological studies on nasopharyngeal carcinoma in China. Advances in Cancer Research 44:121–138
    [Google Scholar]
  27. Zhang C. X., Decaussin G., Tessier M., Daillie J., Ooka T. 1987; Identification of an Epstein-Barr virus-specific des-oxyribonuclease gene using complementary DNA. Nucleic Acids Research 15:2707–2717
    [Google Scholar]
  28. Zhang C. X., Decaussin G., Daillie J., Ooka T. 1988; Altered expression of two Epstein-Barr virus early genes localized in BamRl-A fragment in non producer Raji cells. Journal of Virology 62:1862–1869
    [Google Scholar]
  29. Zur Hausen H., Schulte-Holthausen H., Klein G., Henlé W., Clifford P., Santesson L. 1970; EB-virus DNA in biopsies of Burkitt tumors and anaplastic carcinomas of the nasopharynx. Nature, London 228:1056–1057
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-3-509
Loading
/content/journal/jgv/10.1099/0022-1317-74-3-509
Loading

Data & Media loading...

Most cited Most Cited RSS feed