1887

Abstract

The gene for the homologue of herpesvirus glycoprotein B (gB) has been identified in the genome of human herpesvirus 6 (HHV-6), strain U1102, and the nucleotide sequence was determined. The open reading frame encodes a protein of 830 amino acids (93.2K) with the characteristics of a transmembrane glycoprotein and close similarity to the gp58/116 complex of human cytomegalovirus (HCMV). Monoclonal antibodies 2D10 and 2B9 have been shown previously to react with an HHV-6 glycoprotein of apparent 112K, and its proteolytic cleavage products of 64K and 58K. We show that both monoclonal antibodies detect prokaryotically expressed carboxy-terminal fragments of the HHV-6 gB homologue. This indicates that the HHV-6 gB homologue is probably processed by proteolytic cleavage similar to its equivalents in HCMV and various other herpesviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-3-495
1993-03-01
2021-11-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/3/JV0740030495.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-3-495&mimeType=html&fmt=ahah

References

  1. Albrecht J. C., Fleckenstein B. 1990; Structural organization of the conserved gene block of herpesvirus saimirí coding for DNA polymerase, glycoprotein B, and major DNA binding protein. Virology 174:533–542
    [Google Scholar]
  2. Balachandran N., Amelse R. E., Zhou W. W., Chang C. K. 1989; Identification of proteins specific for human herpesvirus 6-infected human T cells. Journal of Virology 63:2835–2840
    [Google Scholar]
  3. Barr P. J. 1991; Mammalian subtilisins: the long-sought dibasic processing endoproteases. Cell 66:1–3
    [Google Scholar]
  4. Blewett E. L., Misra V. 1991; Cleavage of the bovine herpesvirus glycoprotein B is not essential for its function. Journal of General Virology 72:2083–2090
    [Google Scholar]
  5. Borisch B., Ellinger K., Neipel F., Fleckenstein B., Kirchner T., Ott M. M., Müller-Hermelink H. K. 1991; Lymphadenitis and lymphoproliferative lesions associated with the human herpes virus-6 (HHV-6). Virchows Archiv B: Cell Pathology Including Molecular Pathology 61:179–187
    [Google Scholar]
  6. Borisch-Chappuis B., Ellinger K., Neipel F., Kirchner T., Kujath P., Fleckenstein B., Müller-Hermelink H. K. 1989; Human herpesvirus 6 in lymph nodes. Lancet i:40–41
    [Google Scholar]
  7. Briggs M., Fox J., Tedder R. S. 1988; Age prevalence of antibody to human herpesvirus 6. Lancet i:1058–1059
    [Google Scholar]
  8. Britt W. J., Vugler L. G. 1989; Processing of the gp55-116 envelope glycoprotein complex (gB) of human cytomegalovirus. Journal of Virology 63:403–410
    [Google Scholar]
  9. Cai W., Gu B., Person S. 1988; Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. Journal of Virology 62:2596–2601
    [Google Scholar]
  10. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., III Hutchinson C. A., Kouzarides T., Martignetti J. A., Freddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1990; Analysis of the proteincoding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154:125–169
    [Google Scholar]
  11. Dayhoff M. O., Barker W. C., Hunt L. T. 1983; Establishing homologies in protein sequences. Methods in Enzymology 91:524–545
    [Google Scholar]
  12. Downing R. G., Sewankambo N., Serwadda D., Honess R. W., Crawford D., Jarrett R. F., Griffin B. E. 1987; Isolation of human lymphotropic herpesviruses from Uganda. Lancet 11:105
    [Google Scholar]
  13. Ellinger K., Neipel F., Seidl S., Borisch-Chappuis B., Müller-Hermelink H. K., Fleckenstein B. 1992; Detection of HHV-6 genomes in lymphoproliferative diseases. In Perspectives in Medical Virology, vol. 4, HHV-6 Epidemiology, Molecular Pathology and Clinical Pathology pp. 209–226 Edited by Ablashi D. V., Kriiger G. R., Salahuddin S. Z. Amsterdam: Elsevier;
    [Google Scholar]
  14. Foa-Tomasi L., Boscaro A., di Gaeta S., Campadelli-Fiume G. 1991; Monoclonal antibodies to gplOO inhibit penetration of human herpesvirus 6 and polykaryocyte formation in susceptible cells. Journal of Virology 65:4124–4129
    [Google Scholar]
  15. Foa-Tomasi L., Guerrini S., Huang T., Campadelli-Fiume G. 1992; Characterization of human herpesvirus-6 (U1102) and (GS) gpl 12 and identification of the Z29-specified homolog. Virology (in press)
    [Google Scholar]
  16. Gong M., Kieff E. 1990; Intracellular trafficking of two major Epstein-Barr virus glycoproteins, gp350/220 and gpllO. Journal of Virology 64:1507–1516
    [Google Scholar]
  17. Gong M., Ooka T., Matsuo T., Kieff E. 1987; Epstein-Barr virus glycoprotein homologous to herpes simplex virus gB. Journal of Virology 61:499–508
    [Google Scholar]
  18. Jahn G., Kouzarides T., Mach M., Scholl B. C, Plachter B., Traupe B., Preddie E., Satchwell S. C., Fleckenstein B., Barrell B. G. 1987; Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus. Journal of Virology 61:1358–1367
    [Google Scholar]
  19. Josephs S. F., Buchbinder A., Streicher H. Z., Ablashi D. V., Salahuddin S. Z., Guo H. G., Wong-Staal F., Cossman J., Raffeld M., Sundeen J., Levine P., Biggar R., Krueger G. R., Fox R. I., Gallo R. C. 1988; Detection of human B-lymphotropic virus (human herpesvirus 6) sequences in B cell lymphoma tissues of three patients. Leukemia 2:132–135
    [Google Scholar]
  20. Josephs S. F., Ablashi D. V., Salahuddin S. Z., Jagodzinski L. L., Wong-Staal F., Gallo R. C. 1991; Identification of the human herpesvirus 6 glycoprotein H and putative large tegument protein genes. Journal of Virology 65:5597–5604
    [Google Scholar]
  21. Keller P. M., Davison A. J., Lowe R. S., Bennett C. D., Ellis R. W. 1986; Identification and structure of the gene encoding gpll, a major glycoprotein of varicella-zoster virus. Virology 152:181–191
    [Google Scholar]
  22. Knapp S., Broker M., Amann E. 1990; pSEM vectors : high level expression of antigenic determinants and protein domains. BioTechniques 8:280–281
    [Google Scholar]
  23. Krueger G. R., Manak M., Bourgeois N., Ablashi D. V., Salahuddin S. Z., Josephs S. S., Buchbinder A., Gallo R. C., Berthold F., Tesch H. 1989; Persistent active herpes virus infection associated with atypical polyclonal lymphoproliferation (APL) and malignant lymphoma. Anticancer Research 9:1457–1476
    [Google Scholar]
  24. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  25. Lawrence G. L., Chee M., Craxton M. A., Gompels U. A., Honess R. W., Barrell B. G. 1990; Human herpesvirus 6 is closely related to human cytomegalovirus. Journal of Virology 64:287–299
    [Google Scholar]
  26. Little S. P., Jofre J. T., Courtney R. J., Schaffer P. A. 1981; A virion-associated glycoprotein essential for infectivity of herpes simplex virus type 1. Virology 115:149–160
    [Google Scholar]
  27. Littler E., Lawrence G. L., Liu M. Y., Barrell B. G., Arrand J. R. 1990; Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6. Journal of Virology 64:714–722
    [Google Scholar]
  28. McCune J. M., Rabin L. B., Feinberg M. B., Lieberman M., Kosek J. C., Reyes G. R., Weissman I. R. 1988; Endoproteolytic cleavage of gpl60 is required for the activation of human immunodeficiency virus. Cell 53:55–67
    [Google Scholar]
  29. Mach M., Utz U., Fleckenstein B. 1986; Mapping of the major glycoprotein gene of human cytomegalovirus. Journal of General Virology 67:1461–1467
    [Google Scholar]
  30. Martin M. E. D., Thomson B. J., Honess R. W., Craxton M. A., Gompels U. A., Liu M.-Y., Littler E., Arrand J. R., Tbo I., Jones M. D. 1991; The genome of human herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases. Journal of General Virology 72:157–168
    [Google Scholar]
  31. Nagai K., Thogersen H. C. 1987; Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli. Methods in Enzymology 153:4614–81
    [Google Scholar]
  32. Neipel F., Ellinger K., Fleckenstein B. 1991; The unique region of the human herpesvirus 6 genome is essentially collinear with the UL segment of human cytomegalovirus. Journal of General Virology 72:2293–2297
    [Google Scholar]
  33. Niederman J. C., Liu C. R., Kaplan M. H., Brown N. A. 1988; Clinical and serological features of human herpesvirus-6 infection in three adults. Lancet ii:817–819
    [Google Scholar]
  34. Ohuchi M., Orlich M., Ohuchi R., Simpson B. E., Garten W., Klenk H. D., Rott R. 1989; Mutations at the cleavage site of the hemagglutinin alter the pathogenicity of influenza virus A/chick/Penn/83 (H5N2). Virology 168:274–280
    [Google Scholar]
  35. Okuno T., Takahashi K., Balachandra K., Shiraki K., Yamanishi K., Takahashi M., Baba K. 1989; Seroepidemiology of human herpesvirus 6 infection in normal children and adults. Journal of Clinical Microbiology 27:651–653
    [Google Scholar]
  36. Pellett P. E., Biggin M. D., Barrell B., Roizman B. 1985a; Epstein-Barr virus genome may encode a protein showing significant amino acid and predicted secondary structure homology with glycoprotein B of herpes simplex virus 1. Journal of Virology 56:807–813
    [Google Scholar]
  37. Pellett P. E., Kousoulas K. G., Pereira L., Roizman B. 1985b; Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene : primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants. Journal of Virology 53:243–253
    [Google Scholar]
  38. Pellett P. E., Jenkins F. J., Ackermann M., Sarmiento M., Roizman B. 1986; Transcription initiation sites and nucleotide sequence of a herpes simplex virus 1 gene conserved in the Epstein-Barr virus genome and reported to affect the transport of viral glycoproteins. Journal of Virology 60:1134–1140
    [Google Scholar]
  39. Riggio M. P., Cullinane A. A., Onions D. E. 1989; Identification and nucleotide sequence of the glycoprotein gB gene of equine herpesvirus 4. Journal of Virology 63:1123–1133
    [Google Scholar]
  40. Robbins A. K., Dorney D. J., Wathen M. W., Whealy M. E., Gold C., Watson R. J., Holland L. E., Weed S. D., Levine M., Glorioso J. C., Enquist L. W. 1987; The pseudorabies virus gil gene is closely related to the gB glycoprotein gene of herpes simplex virus. Journal of Virology 61:2691–2701
    [Google Scholar]
  41. Salahuddin S. Z., Ablashi D. V., Markham P. D., Josephs S. F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-Staal F., Kramarsky B., Gallo R. C. 1986; Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science 234:596–601
    [Google Scholar]
  42. Spaete R. R., Thayer R. M., Probert W. S., Masiarz F. R., Chamberlain S. H., Rasmussen L., Merigan T. C., Pachl C. 1988; Human cytomegalovirus strain Towne glycoprotein B is processed by proteolytic cleavage. Virology 167:207–225
    [Google Scholar]
  43. Teo I. A., Griffin B. E., Jones M. D. 1991; Characterization of the DNA polymerase gene of human herpesvirus 6. Journal of Virology 65:4670–4680
    [Google Scholar]
  44. Whitbeck J. C., Bello L. J., Lawrence W. C. 1988; Comparison of the bovine herpesvirus 1 gl gene and the herpes simplex virus type 1 gB gene. Journal of Virology 62:3319–3327
    [Google Scholar]
  45. WuDunn D., Spear P. G. 1989; Initial interaction of herpes simplex virus with cells: binding to heparan sulfate. Journal of Virology 63:52–58
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-3-495
Loading
/content/journal/jgv/10.1099/0022-1317-74-3-495
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error