@article{mbs:/content/journal/jgv/10.1099/0022-1317-74-3-471, author = "Brideau, R. J. and Oien, N. L. and Lehman, D. J. and Homa, F. L. and Wathen, M. W.", title = "Protection of cotton rats against human parainfluenza virus type 3 by vaccination with a chimeric FHN subunit glycoprotein", journal= "Journal of General Virology", year = "1993", volume = "74", number = "3", pages = "471-477", doi = "https://doi.org/10.1099/0022-1317-74-3-471", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-74-3-471", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "A cotton rat model of experimental human parainfluenza virus type 3 (PIV-3) infection was used to examine the efficacy of FHN, a novel chimeric glycoprotein which contains the extracellular regions of the fusion (F) and haemagglutinin—neuraminidase (HN) glycoproteins of PIV-3. The FHN protein was expressed in insect cells using a baculovirus vector system. FHN vaccination resulted in induction of neutralizing antibodies, was completely protective at doses of 100 ng, and was superior to vaccination with secreted forms F and HN proteins, or mixtures of the F and HN glycoproteins. In addition, FHN immunization induced lymphoproliferative responses in mice which were directed against both the F and HN glycoproteins. Fusion of the F and HN proteins into a single chimeric glycoprotein appeared to enhance the protective immune response compared to that elicited by the individual glycoproteins or mixtures of the two glycoproteins.", }