%0 Journal Article %A Coulter, L. J. %A Moss, H. W. M. %A Lang, J. %A McGeoch, D. J. %T A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted %D 1993 %J Journal of General Virology, %V 74 %N 3 %P 387-395 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-74-3-387 %I Microbiology Society, %X Gene UL13 of herpes simplex virus type 1 (HSV-1) has previously been proposed to encode a protein kinase. An HSV-1 mutant with UL13 inactivated by insertion of the Escherichia coli lacZ gene was constructed. This UL13-lacZ mutant was found to grow to near wild-type (wt) titres in tissue culture. Comparison of silver-stained SDS-PAGE profiles of wt and UL13-lacZ virions demonstrated that the UL13 protein is a readily detectable component of wt virions, located in the tegument and probably equivalent to the previously described species VP18.8. Studies of in vitro phosphorylation with nuclear extracts of virus-infected cells and with detergent-treated virions showed that the UL13 protein is involved in phosphorylation of the tegument protein VP22. Extracts of cells engineered to express UL13, and infected with UL13-lacZ virus, were also capable of VP22 phosphorylation. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-74-3-387