1887

Abstract

The immunoreactivity of a panel of polyclonal anti-bodies and monoclonal antibodies (MAbs) raised against African isolates of potyviruses from cowpea and African yam bean was examined in ELISAs. A serological study including reference isolates followed by further characterization in differential hosts resulted in separation of the potyviruses into two distinct serogroups, one containing blackeye cowpea mosaic virus (BlCMV) and the other containing cowpea aphid-borne mosaic virus (CAMV). Using biotin-labelled MAbs, the BlCMV isolates were further subdivided into two serotypes and the CAMV isolates into five serotypes. Because both BlCMV and CAMV induce a very similar mosaic disease in cowpea, different ELISA procedures using mixed MAbs were evaluated and a single protocol was developed which allowed reliable diagnosis of both viruses.

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1993-03-01
2021-10-26
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References

  1. Atiri G. I., Enobakhare D. A., Thottappilly G. 1986; The importance of colonizing and non-colonizing aphid vectors in the spread of cowpea aphid-borne mosaic virus in cowpea. Crop Protection 5:406–410
    [Google Scholar]
  2. Behncken G. M., Maleevsky L. 1977; Detection of cowpea aphid-borne mosaic virus in Queensland. Australian Journal of Experimental Agriculture and Animal Husbandry 17:674–678
    [Google Scholar]
  3. Bock K. R. 1973; East African strains of cowpea aphid-borne mosaic virus. Annals of Applied Biology 74:75–83
    [Google Scholar]
  4. Bos L. 1970; The identification of three new viruses isolated from wisteria and pisum in The Netherlands, and the problem of variation within the potato virus Y group. Netherlands Journal of Plant Pathology 76:8–46
    [Google Scholar]
  5. Dekker E. L., Dore I., Porta C., Van Regenmortel M. H. V. 1987; Conformational specificity of monoclonal antibodies used in the diagnosis of tomato mosaic virus. Archives of Virology 94:191–203
    [Google Scholar]
  6. Dijkstra J., Bos L., Bouwmeester H. J., Hadiastono T., Lohuis H. 1987; Identification of blackeye cowpea mosaic virus from germplasm of yard-long bean and from soybean, and the relationships between blackeye cowpea mosaic virus and cowpea aphid-borne mosaic virus. Netherlands Journal of Plant Pathology 93:115–133
    [Google Scholar]
  7. Fischer H. U., Lockhart B. E. 1976; A strain of cowpea aphid-borne mosaic virus isolated from cowpeas in Morocco. Phytopathologische Zeitschrift 85:43–48
    [Google Scholar]
  8. Huguenot C., Givord L., Sommermeyer G., Van Regenmortel M. H. V. 1989; Differentiation of peanut clump virus serotypes by monoclonal antibodies. Research in Virology 140:87–102
    [Google Scholar]
  9. Iwaki M., Roechan M., Tantera D. M. 1975; Virus diseases of legume plants in Indonesia, 1. Cowpea aphid-borne mosaic virus. Contributions of the Central Research Institute for Agriculture, Bogor 13:1–14
    [Google Scholar]
  10. Kannangara T., Wieczorek A., Lavender D. P. 1989; Immunoaffinity columns for the analysis of abscisic acid in conifer seedlings. Physiologia Plantarum 75:369–373
    [Google Scholar]
  11. Lima J. A. A., Purcifull D. E., Hiebert E. 1979; Purification, partial characterization, and serology of blackeye cowpea mosaic virus. Phytopathology 69:1252–1258
    [Google Scholar]
  12. Lovisolo O., Contí M. 1966; Identification of an aphid-transmitted cowpea mosaic virus. Netherlands Journal of Plant Pathology 72:265–269
    [Google Scholar]
  13. Mali V. R., Kulthe K. S. 1980; A seedborne potyvirus causing mosaic of cowpea in India. Plant Disease 64:925–928
    [Google Scholar]
  14. Pio-Ribeiro G., Kuhn C. W. 1980; Cowpea stunt: heterogeneous and differential reaction of cowpea cultivars. Phytopathology 70:244–249
    [Google Scholar]
  15. Prowidenti R., Gonsalves D., Taiwo M. A. 1983; Inheritance of resistance to blackeye cowpea mosaic and cowpea aphid-borne mosaic virus in Phaseolus vulgaris. Journal of Heredity 74:60–61
    [Google Scholar]
  16. Purcifull D., Gonsalves D. 1985; Blackeye cowpea mosaic virus. CMI/AAB Description of Plant Viruses no 305
    [Google Scholar]
  17. Rossel H. W., Thottappilly G. 1985; Virus diseases of important food crops in tropical Africa. International Institute of Tropical Agriculture Publication Series Ibadan: Nigeria;
    [Google Scholar]
  18. Taiwo M. A., Gonsalves D. 1982; Serological grouping of isolates of blackeye cowpea mosaic and cowpea aphidborne mosaic viruses. Phytopathology 72:583–589
    [Google Scholar]
  19. Taiwo M. A., Gonsalves D., Provvidenti R., Thurston H. D. 1982; Partial characterization and grouping of isolates of blackeye cowpea mosaic and cowpea aphidborne mosaic viruses. Phytopathology 72:590–596
    [Google Scholar]
  20. Zettler F. W., Evans I. R. 1972; Blackeye cowpea mosaic virus in Florida: host range and incidence in certified cowpea seed. Proceedings of the Florida State Horticultural Society 85:99–101
    [Google Scholar]
  21. Zettler G. L, Christie R. G., Edwardson J. R. 1967; Aphid transmission of virus from leaf sectors correlated with intracellular inclusions. Virology 33:549–552
    [Google Scholar]
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