1887

Abstract

Transgenic tobacco expressing the coat protein (CP) of cucumber mosaic virus strain O (CMV-O) showed a significant level of protection against CMV strains O and Y. When inoculum concentrations were increased, the transformants showed a stronger level of protection against CMV-O than against CMV-Y. The substitution of an amino acid residue between CMV-O and CMV-Y, which is presumed to change the conformation of CP, may reflect the difference in susceptibility to these viruses. Furthermore the transgenic tobacco plants showed a significant level of protection against chrysanthemum mild mottle virus, a member of the cucumovirus group but with no serological relationship to CMV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-2-319
1993-02-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/2/JV0740020319.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-2-319&mimeType=html&fmt=ahah

References

  1. An G. 1986; Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells. Plant Physiology 81:86–91
    [Google Scholar]
  2. Beachy R. N., Loesch-Fries S., Tumer N. E. 1990; Coat protein-mediated resistance against virus infection. Annual Review of Phytopathology 28:451–474
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  4. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  5. Fling M. E., Kopf J., Richards C. 1985; Nucleotide sequence of the transposon Tn7 gene encoding an aminoglycoside modifying enzyme, 3'(9)-nucleotidyltransferase. Nucleic Acids Research 13:7095–7106
    [Google Scholar]
  6. Garnier J., Osguthorpe D. J., Robson B. 1978; Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. Journal of Molecular Biology 120:97–120
    [Google Scholar]
  7. Hanada K., Tochihara H. 1980; Genetic analysis of cucumber mosaic, peanut stunt and chrysanthemum mild mottle viruses. Annals of the Phytopathological Society of Japan 46:159–168
    [Google Scholar]
  8. Hayakawa T., Mizukami M., Nakamura I., Suzuki M. 1989a; Cloning and sequencing of RNA-1 cDNA from cucumber mosaic virus strain o. Gene 85:533–540
    [Google Scholar]
  9. Hayakawa T., Mizukami M., Nakajima M., Suzuki M. 1989b; Complete nucleotide sequence of RNA 3 from cucumber mosaic virus (CMV) strain O: comparative study of nucleotide sequences and amino acid sequences among CMV strains O, Q, D and Y. Journal of General Virology 70:499–504
    [Google Scholar]
  10. Hood E. E., Helmer G. L., Fraley R. T., Chilton M.-D. 1986; The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. Journal of Bacteriology 168:1291–1301
    [Google Scholar]
  11. Hofgen R., Willmitzer L. 1988; Storage of competent cells for Agrobacterium transforation. Nucleic Acids Research 16:9877
    [Google Scholar]
  12. Jefferson R. A. 1987; Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Reporter 5:387–405
    [Google Scholar]
  13. Ling K., Namba S., Gonsalves C., Slightom L., Gonsalves D. 1991; Protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the papaya ringspot virus coat protein gene. Bio/Technology 9:752–758
    [Google Scholar]
  14. Linsmaier E. M., Skoog F. 1965; Organic growth factor requirements of tobacco tissue cultures. Physiologia plantarum 18:100–127
    [Google Scholar]
  15. Loesch-Fries L. S., Merlo D., Zinnen T., Burhop L., Hill K., Krahn K., Jarvis N., Nelson S., Halk E. 1987; Expression of alfalfa mosaic virus RNA 4 in transgenic plants confers virus resistance. EMBO Journal 6:1845–1851
    [Google Scholar]
  16. Maliga P., Sz.-Breznovits A., Marton L. 1973; Streptomycin-resistant plants from callus culture of haploid tobacco. Nature New Biology 244:29–30
    [Google Scholar]
  17. Murashige T., Skoog F. 1962; A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia plantarum 15:473–497
    [Google Scholar]
  18. Namba S., Ling K., Gonsalves C., Gonsalves D., Slightom J. 1991; Expression of the gene encoding the coat protein of cucumber mosaic virus (CMV) strain WL appears to provide protection to tobacco plants against infection by several different CMV strains. Gene 107:181–188
    [Google Scholar]
  19. Nejidat A., Beachy R. N. 1990; Transgenic tobacco plants expressing a coat protein gene of tobacco mosaic virus are resistant to some other tobamoviruses. Molecular Plant-Microbe Interactions 3:247–251
    [Google Scholar]
  20. Nitta N., Masuta C., Kuwata S., Takanami Y. 1988; Comparative studies on the nucleotide sequence of cucumber mosaic virus RNA 3 between Y strain and Q strain. Annals of the Phytopathological Society of Japan 54:516–522
    [Google Scholar]
  21. Perbal B. 1988; Detection of polypeptide synthesis by immunoblotting. In A Practical Guide to Molecular Cloning pp 792–794 New York: John Wiley & Sons;
    [Google Scholar]
  22. Powell Abel P., Nelson R. S., De B., Hoffmann N., Rogers S. G., Fraley R. T., Beachy R. N. 1986; Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–743
    [Google Scholar]
  23. Quemada H. D., Gonsalves D., Slightom J. L. 1991; Expression of coat protein gene from cucumber mosaic virus strain C in tobacco: protection against infection by CMV strains transmitted mechanically and by aphids. Phytopathology 81:794–802
    [Google Scholar]
  24. Register J. C. III, Beachy R. N. 1988; Resistance to TMV in transgenic plants results from interference with an early event in infection. Virology 166:524–532
    [Google Scholar]
  25. Richards E. 1987; Preparation of genomic DNA from plant tissue. In Current Protocols in Molecular Biology, Unit 2,3 Edited by Ausubej F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: John Wiley & Sons;
    [Google Scholar]
  26. Stark D. M., Beachy R. N. 1989; Protection against potyvirus infection in transgenic plants: evidence for broad spectrum resistance. Bio/Technology 7:1257–1262
    [Google Scholar]
  27. Tochihara H. 1970; Some properties of chrysanthemum mild mottle virus, and comparison of this virus with cucumber mosaic virus. Annals of the Phytopathological Society of Japan 36:1–10
    [Google Scholar]
  28. Tumer N. E., Kaniewski W., Haley L., Gehrkb L., Lodge J. K., Sanders P. 1991; The second amino acid of alfalfa mosaic virus coat protein is critical for protein-mediated protection. Proceedings of the National Academy of Sciences, U.S.A 882331–2335
    [Google Scholar]
  29. Van Dun C. M. P., Bol J. F. 1988; Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167:649–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-2-319
Loading
/content/journal/jgv/10.1099/0022-1317-74-2-319
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error