1887

Abstract

We have cloned several cDNAs derived from the P gene of rinderpest virus. One of these, derived from a bicistronic N-P mRNA, has been sequenced in its entirety. Sequencing of a section of the others, and comparison with the genome sequence, showed that P gene transcripts, as for other morbilliviruses, were variable; non-templated Gs could be added at a site resembling the normal stop transcription site. Primer extension analysis showed that about half the transcripts were edited. Sequences of the P, C and V proteins encoded by the normal and edited transcripts were compared with those of other morbilliviruses and with those of the more distantly related paramyxoviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-2-299
1993-02-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/2/JV0740020299.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-2-299&mimeType=html&fmt=ahah

References

  1. Barrett T., Shrimpton S. B., Russell S. E. H. 1985; Nucleotide sequence of the entire protein coding region of the canine distemper virus polymerase-associated (p) protein mRNA. Virus Research 3:367–3727
    [Google Scholar]
  2. Barrett T., Subbarao S. M., Belsham G. J., Mahy B. W. J. 1989; The molecular biology of the morbilliviruses. In The Paramyxoviruses pp 83–102 Edited by Kingsbury D. W. New York: Plenum Press;
    [Google Scholar]
  3. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus p gene codes for two proteins. Journal of Virology 53:908–919
    [Google Scholar]
  4. Berg M., Hjertner B., Moreno-López J., Linné T. 1992; The P gene of the porcine paramyxovirus LPMV encodes three possible polypeptides p, V and C: the P protein mRNA is edited. Journal of General Virology 73:1195–1200
    [Google Scholar]
  5. Blixenkrone-Möller M., Sharma B., Varsanyi T. M., Hu A., Norrby E., Kovamees J. 1992; Sequence analysis of the genes encoding the nucleocapsid protein and phosphoprotein (P) of phocid distemper virus, and editing of the p gene transcript. Journal of General Virology 73:885–893
    [Google Scholar]
  6. Cattaneo R., Kaelin K., Baczko K., Billeter M. A. 1989; Measles virus editing provides an additional cyteine-rich protein. Cell 56:759–764
    [Google Scholar]
  7. Collins J. F., Coulson A. F. W. 1987; Molecular sequence comparison and alignment. In Nucleic Acid and Protein Sequence Analysis: A Practical Approach pp 323–358 Edited by Bishop M. J., Rawlings C. J. Oxford: IRL Press;
    [Google Scholar]
  8. Crowley J. C., Dowling P. C., Menonna J., Silverman J. I., Schuback D., Cook S. D., Blumberg B. M. 1988; Sequence variability and function of measles virus 3' and 5' ends and intergenic region. Virology 164:498–506
    [Google Scholar]
  9. Curran J., Boeck R., Kolakofsky D. 1991a; The Sendai virus P gene expresses both an essential protein and an inhibitor of RNA synthesis by shuffling modules via mRNA editing. EMBO Journal 10:3079–3085
    [Google Scholar]
  10. Curran J., de Melo M., Moyer S., Kolakofsky D. 1991b; Characterization of the Sendai virus V protein with an anti-peptide antiserum. Virology 184:108–116
    [Google Scholar]
  11. Curran J., Marq J.-B., Kolakofsky D. 1992; The Sendai virus non-structural proteins specifically inhibit viral mRNA synthesis. Virology 189:647–656
    [Google Scholar]
  12. Curran M. D., Rima B. K. 1992; The genes encoding the phospho- and matrix proteins of phocine distemper virus. Journal of General Virology 73:1587–1591
    [Google Scholar]
  13. Curran M. D., Oxoan D., Kennedy S., Rima B. K. 1992; Molecular characterization of the phocine distemper virus: gene order and sequence of the gene encoding the attachment (H) protein. Journal of General Virology 73:1189–1194
    [Google Scholar]
  14. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  15. Driscoll D. M., Wynne J. K., Wallis S. C., Scott J. 1989; An in vitro system for the editing of apolipoprotein B mRNA. Cell 58:519–525
    [Google Scholar]
  16. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  17. Feinberg A. P., Vogelstein B. 1984; Addendum: a technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  18. Galinski M. S., Troy R. M., Banerjee A. K. 1992; RNA editing in the phosphoprotein gene of the human parainfluenza virus type 3. Virology 186:543–550
    [Google Scholar]
  19. Gombart A. F., Hirano A., Wong T. W. 1992; Expression and properties of the V protein in acute measles virus and subacute sclerosing panencephalitis virus strains. Virus Research 25:63–78
    [Google Scholar]
  20. Huber M., Cattaneo. R., Spielhofer. P., Örvell C., Norrby E., Messerli M., Perriard J.-C., Billeter M. A. 1991; Measles virus phosphoprotein retains the nucleocapsid protein in the cytoplasm. Virology 185:299–308
    [Google Scholar]
  21. Matsuoka Y., Curran J., Pelet T., Kolakofsky D., Ray R., Compans R. W. 1991; The P gene of human parainfluenza virus type 1 encodes P and C proteins but not a cysteine-rich V protein. Journal of Virology 65:3406–3410
    [Google Scholar]
  22. Ohgimoto S., Bando H., Kawano M., Okamoto K., Kondo K., Tsurudome M., Nishio M., Ito Y. 1990; Sequence analysis of P gene of human parainfluenza type 2 virus: P and cysteine-rich proteins are translated by two mRNAs that differ by two nontemplated G residues. Virology 177:116–123
    [Google Scholar]
  23. Pandey K. D., Baron M. D., Barrett T. 1992; Use of biotinylated cDNA probes in differential diagnosis of rinderpest (RP) and peste des petits ruminants (PPR). Veterinary Record 131:199–200
    [Google Scholar]
  24. Paterson. R. G., Lamb R. A. 1990; RNA editing by G-nucleotide insertion in mumps virus P-gene mRNA transcripts. Journal of Virology 64:4137–4145
    [Google Scholar]
  25. Pelet. T., Curran J., Kolakofsky. D. 1991; The P gene of bovine parainfluenza virus 3 expresses all three reading frames from a single mRNA editing site. EMBO Journal 10:443–448
    [Google Scholar]
  26. Takeuchi K., Tanabayashi K., Hishiyama M., Yamada Y. K., Yamada A., Sugiura A. 1992; Detection and characterization of mumps virus V protein. Virology 178:247–253
    [Google Scholar]
  27. Thomas S. M., Lamb R. A., Paterson R. G. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  28. Wardrop E. A., Briedis D. J. 1991; Characterization of V protein in measles virus-infected cells. Journal of Virology 65:3421–3428
    [Google Scholar]
  29. Windle B. E. 1988; A simple technique for preparing pure λ DNA. Biotechniques 6:403–408
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-74-2-299
Loading
/content/journal/jgv/10.1099/0022-1317-74-2-299
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error