1887

Abstract

We studied the effect of two cytokines, basic fibroblastic growth factor (bFGF) and transforming growth factor beta (TGF-) on human cytomegalovirus (HCMV) replication in cultured human lung fibroblasts. We show that TGF- increases HCMV production, probably by a transcriptional mechanism, and that bFGF represses HCMV replication in a dose-dependent manner. These actions were antagonistic and the mechanisms involved were independent of the effects of these factors on cell DNA synthesis and proliferation.

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1993-02-01
2024-04-19
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References

  1. Albrecht T., Boldogh I., Fons M., Lee C. H., AbuBakar S. N., Russell J. M., Au W. 1989; Cell activation responses to cytomegalovirus infection. Relationship to the phasing of CMV replication and to the induction of cellular damage. In Subcellular Biochemistry pp 157–202 Edited by Harris J. R. New York: Plenum Press;
    [Google Scholar]
  2. Alcami J., Barzu T., Michelson S. 1991; Induction of an endothelial cell growth factor by human cytomegalovirus infection of fibroblasts. Journal of General Virology 12:2765–2770
    [Google Scholar]
  3. Assoian R. K., Fleurdelys B. E., Stevenson H. C., Miller P. J., Madtcs D. K., Raines E. W., Ross R., Sporn M. B. 1987; Expression and secretion of type b transforming growth factor by activated human macrophages. Proceedings ofthe National Academy of Sciences, U.S.A 846020–6024
    [Google Scholar]
  4. Baird A., Florkiewicz R. Z., Maher P. A., Kaner R. J., Hajjar D. P. 1990; Mediation of virion penetration into vascular cells by association of basic fibroblast growth factor with herpes simplex virus type I. Nature, London 348:344–346
    [Google Scholar]
  5. Birchenall-Roberts M. C., Ruscetti F. W., Kasper J., Lee H. D., Friedman R., Geiser A., Sporn M. B., Roberts A. B., Kim S.-J. 1990; Transcriptional regulation ofthe transforming growth factor beta 1 promoter by v-src gene product is mediated through the API-complex. Molecular and Cellular Biology 10:4978–4983
    [Google Scholar]
  6. Boldogh I., AbuBakar S., Albrecht T. 1990; Activation of proto-oncogenes: an immediate early event in human cyto-megalovirus infection. Science 247:561–564
    [Google Scholar]
  7. Chambard J. C., Pouyssegur J. 1988; TGF-β inhibits growth factor-induced DNA synthesis in hamster fibroblast without affecting the early mitogenic events. Journal of Cellular Physiology 135:101–107
    [Google Scholar]
  8. Davis L. G., Dibner M. D., Battey J. F. 1986 Basic Methods in Molecular Biology New York, Amsterdam & London: Elsevier;
    [Google Scholar]
  9. Gönczöl E., Andrews P. W., Plotkin S. A. 1984; Cytomega-lovirus replicates in differentiated but not in undifferentiated human embyonal carcinoma cells. Science 224:159–161
    [Google Scholar]
  10. Gospodarowicz D., Ferrara N., Schzeigerer L., Neufeld G. 1987; Structural characterization and biological functions of fibroblast growth factor. Endocrine Review 8:95–114
    [Google Scholar]
  11. Hall S. H., Berthelon M. C., Avallet O., Saez J. M. 1991; Regulation of c-fos, c-jun, jun B and c-myc messenger ribonucleic acids by gonadotropin and growth factors in cultured pig Leydig cell. Endocrinology 129:1243–1249
    [Google Scholar]
  12. Hennighausen L., Fleckenstein B. 1986; Nuclear factor 1 interacts with five DNA elements in the promoter region of the human cytomegalovirus major immediate-early gene. EMBO Journal 5:1367–1371
    [Google Scholar]
  13. Hill D. J., Strain A. J., Hlstow S. F., Swenne I., Milner R. D. 1986; Bi-functional action of transforming growth factor-b on DNA synthesis in early passage human fetal fibroblasts. Journal of Cellular Physiology 128:322–328
    [Google Scholar]
  14. Hirt R. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  15. Hunnighake G. W., Monick M. M., Liu B., Stinski M. F. 1989; The promoter-regulatory region of the major immediate-early gene of human cytomegalovirus responds to T-lymphocyte stimulation and contains functional cyclic AMP-response elements. Journal of Virology 63:3026–3033
    [Google Scholar]
  16. Ibanez C. E., Schrier R., Ghazal P., Wiley C., Nelson J. A. 1991; Human cytomegalovirus productively infects differentiated macrophages. Journal of Virology 65:6581–6588
    [Google Scholar]
  17. Jacobs J. P., Jones C. M., Baille J. P. 1970; Characteristics of a human diploid cell designated MRC-5. Nature, London 227:168–170
    [Google Scholar]
  18. Jeang K., Rawlins D. R., Rosenfeld P. J., Shero J. H., Kelly T. J., Hazard G. S. 1987; Multiple tandemly repeated bidding sites for cellular nuclear factor 1 that surround the major immediate-early promoters of simian and human cytomegalovirus. Journal of Virology 61:1559–1570
    [Google Scholar]
  19. Kaner R. J., Baird A., Mansukhani A., Basilico C., Summers B. P., Florkiewicz R. Z., Hajjar D. P. 1990; Fibroblast growth factor receptor is a portal of cellular entry for herpes simplex virus type 1. Science 248:1410–1413
    [Google Scholar]
  20. Kehrl J. H., Wakefield L. M., Roberts A. B., Jakowlew S. B., Alvarez-Mon M., Sporn M. B., Fauci A. S. 1986; Production of transforming growth factor b by human T lymphocytes and its potential role in the regulation of T cell growth. Journal of Experimental Medicine 166:1037–1050
    [Google Scholar]
  21. Kim S.-J., Jeang K.-T., Glick A. B., Sporn M. B., Roberts A. B. 1989a; Promoter sequences of the human transforming growth factor-bl gene responsive to transforming growth factor-bl autoinduction. Journal of Biological Chemistry 264:7041–7045
    [Google Scholar]
  22. Kim S.-J., Denhez F., Kim K. Y., Holt J. T., Sporn M. B., Roberts A. B. 1989b; Activation of the second promoter of the transforming growth factor-beta 1 gene by transforming growth-factor beta 1 and phorbol ester occurs through the same target sequences. Journal of Biological Chemistry 264:19373–19378
    [Google Scholar]
  23. Kim S.-J., Angel P., Lafyatis R., Hattori K., Kim K. Y., Sporn M. B., Karin M., Roberts A. B. 1989c; Auto-induction of TGF-β1 is mediated by the AP-1 complex. Molecular and Cellular Biology 10:1492–1497
    [Google Scholar]
  24. Kramer I. M., Koornneef I., Laat S. W., Eijnden-van Raau A. J. M. 1991; TGF-β1 induces phosphorylation of the cyclic AMP responsive element binding protein in ML-CCL64 cells. EMBO Journal 10:1083–1089
    [Google Scholar]
  25. Kuan-Teh J., Rawlins D. R., Rosenfeld P. J., Shero J. H., Kelly T. J., Haywakd G. S. 1987; Multiple tandemly repeated binding sites for cellular nuclear factor 1 that surround the major immediate-early promoters of simian and human cytomegalovirus’. Journal of Virology 61:1559–1570
    [Google Scholar]
  26. LaFemina R., Hayward G. S. 1986; Constitutive and retinoic acid-inducible expression of cytomegalovirus immediate-early genes in human teratocarcinoma cells. Journal of Virology 58:434–440
    [Google Scholar]
  27. Moses H. L., Yang E. Y., Pietenpol J. A. 1990; TGF-β stimulation and inhibition of cell proliferation: new mechanistic insights. Ceil63247–245
    [Google Scholar]
  28. Pertovaara L., Sistonen L., Bos T. J., Vogt P. K., Keski-Oja J., Alitalo K. 1989; Enhanced jun gene expression is an early genomic response to transforming growth factor b stimulation. Molecular and Cellular Biology 9:1255–1262
    [Google Scholar]
  29. Roberts A. B., Sporn M. B. 1988; Transforming growth factor b. Advances in Cancer Research 51:107–145
    [Google Scholar]
  30. Roberts A. B., Anzamo M. A., Wakefield L. M., Roche N. S., Stern D. F., Sporn M. B. 1985; Type beta transforming growth factor: a bifunctional regulator of cellular growth. Proceedings ofthe National Academy of Sciences, U.S.A 82119–123
    [Google Scholar]
  31. Rossi P., Karsenty G., Roberts A. B., Roche N. S., Sporn M. B., de Crombrugghe B. 1988; A nuclear factor 1 binding site mediates the transcriptional activation of type I collagen promoter by transforming growth factor-b. Cell 52:405–414
    [Google Scholar]
  32. St Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. 1974; Stimulation of cellular DNA synthesis by human cytomegalovirus. Journal of Virology 13:353–362
    [Google Scholar]
  33. Sambucetti L. C., Carrington J. M., Wilkinson G. W. G., Mocarski E. 1989; NF-kB activation of the cytomegalovirus enhancer is mediated by a viral transactivator and by T cell stimulation. EMBO Journal 8:4251–4258
    [Google Scholar]
  34. Schwartz O., Virelizier J. L., Montagnier L., Hazan U. 1990; A microtransfection method using luciferase gene for the study of HIV LTR activity. Gene 88:197–205
    [Google Scholar]
  35. Shelbourn S. L., Sissons J. G. P., Sinclair J. H. 1989; Expression of oncogenic ras in human teratocarcinoma cells induces partial differentiation and permissiveness for human cytomega-lovirus infection. Journal of General Virology 70:367–374
    [Google Scholar]
  36. Stinski M. F., Roehr T. J. 1985; Activation of the major immediate early gene of human cytomegalovirus by cis-acting elements in the promoter-regulatory sequence and by virus-specific trans-acting components. Journal of Virology 55:431–441
    [Google Scholar]
  37. Takehara K., Leroy E. C., Grotendorst G. R. 1987; TGF-β inhibition of endothelial cell proliferation: alteration of EGF binding and EGF-induced growth-regulatory (competence) gene expression. Cell 49:415–422
    [Google Scholar]
  38. Wahl S. M., Hunt D. A., Wakjefield L. M., McCartney-Francis N., Wahl L. M., Roberts A. B., Sporn M. B. 1987; Transforming growth factor type b induces monocyte chemotaxis and growth factor production. Proceedings of the National Academy of Sciences, U.S-A 845788–5792
    [Google Scholar]
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